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AGBL2 Monoclonal Antibody

  • 货号：
    CSB-MA732978A0m
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate
      All lanes: AGBL2 antibody at 1:1000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 32 KDa
      Observed band size: 32, 49, 104 KDa
      Exposure time：5min
  • 其他：

产品详情

  • 产品描述：

    The AGBL2 monoclonal antibody is generated using hybridoma technology. Specifically, mice were immunized with the recombinant human cytosolic carboxypeptidase 2 protein (10-270aa), and B cells were extracted from their spleens. These B cells were then fused with myeloma cells to create hybridomas, from which AGBL2 antibody-producing hybridomas were selected. The selected hybridomas were cultured in the mouse abdominal cavity, and the AGBL2 monoclonal antibodies were purified from mouse ascites using affinity chromatography. The purified antibody is a mouse monoclonal antibody that reacts specifically with human AGBL2 protein in ELISA and WB applications.

    AGBL2, also called cytosolic carboxypeptidase 2 (CCP2), is a member of the AGBL protein family that plays a crucial role in regulating cell proliferation and apoptosis. AGBL2 has been implicated in various cellular processes such as protein degradation, signal transduction, and cytoskeletal reorganization. It also plays a role in the regulation of autophagy and the stability of the microtubule cytoskeleton.

  • 产品名称：
    Mouse anti-Homo sapiens (Human) AGBL2 Monoclonal Antibody antibody
  • Uniprot No.：
    Q5U5Z8
  • 基因名：
    AGBL2
  • 别名：
    AGBL 2 antibody; AGBL2 antibody; ATP/GTP binding protein like 2 antibody; ATP/GTP-binding protein-like 2 antibody; Carboxypeptidase 2 cytosolic antibody; CBPC2_HUMAN antibody; CCP2 antibody; Cytosolic carboxypeptidase 2 antibody; FLJ23598 antibody
  • 宿主：
    Mouse
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Cytosolic carboxypeptidase 2 protein (10-270AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal Antibody
  • 抗体亚型：
    IgG2b
  • 纯化方式：
    Affinity purification
  • 克隆号：
    1F3C12
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Metallocarboxypeptidase that mediates deglutamylation of tubulin and non-tubulin target proteins. Catalyzes the removal of polyglutamate side chains present on the gamma-carboxyl group of glutamate residues within the C-terminal tail of tubulin protein. Specifically cleaves tubulin long-side-chains, while it is not able to remove the branching point glutamate. Also catalyzes the removal of polyglutamate residues from the carboxy-terminus of non-tubulin proteins such as MYLK.
  • 基因功能参考文献：
    1. Findings suggest that ATP/GTP binding protein like 2 (AGBL2) plays a critical oncogenic role in the pathogenesis of hepatocellular carcinoma (HCC) through modulation on immunity-related GTPase family, M protein (IRGM)-regulated autophagy and aurora kinase A (Aurora A) activity. PMID: 29126912
    2. Suggest role for AGBL2 in cell proliferation and chemotherapy resistance in gastric cancer. PMID: 25916089
    3. High AGBL2 expression is associated with breast cancer. PMID: 24884516
    4. RARRES1, its interacting partners AGBL2, Eg5/KIF11, another EEY-bearing protein (EB1), and the microtubule tyrosination cycle are important in tumorigenesis. PMID: 21303978
  • 亚细胞定位：
    Cytoplasm, cytosol. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm, cytoskeleton, cilium basal body.
  • 蛋白家族：
    Peptidase M14 family
  • 数据库链接：

    HGNC: 26296

    OMIM: 617345

    KEGG: hsa:79841

    STRING: 9606.ENSP00000298861

    UniGene: Hs.147377