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CYC1 Monoclonal Antibody

  • 货号：
    CSB-MA006327A0m
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: CYC1 antibody at 1:1000
      Lane 1: Hela whole cell lysate
      Lane 2: HepG2 whole cell lysate
      Lane 3: MCF-7 whole cell lysate
      Lane 4: K562 whole cell lysate
      Lane 5: U251 whole cell lysate
      Lane 6: A549 whole cell lysate
      Lane 7: NIH/3T3 whole cell lysate
      Lane 8: PC-3 whole cell lysate
      Secondary
      Goat polyclonal to Mouse IgG at 1/20000 dilution
      Predicted band size: 35 KDa
      Observed band size: 35 KDa
      Exposure time: 5min
    • Overlay Peak curve showing HepG2 cells stained with CSB-MA006327A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • 产品描述：

    Mice were immunized with the recombinant human CYC1 protein (85-325aa), followed by the isolation of B cells from the mouse spleen, the fusion of these B cells with myeloma cells, and the selection of hybridomas that produce CYC1 antibodies. The CYC1 antibody-generating hybridomas were cultured to collect the CYC1 monoclonal antibodies. The CYC1 monoclonal antibody specifically reacts with human and mouse species and is suitable for ELISA, WB, and FC applications. The antibody is purified using protein A affinity chromatography and has a purity of over 95%.

    CYC1 plays a crucial role in cellular energy metabolism and cell death regulation. It participates in the transfer of electrons between complexes III and IV of the respiratory chain, which drives the proton gradient across the mitochondrial inner membrane necessary for ATP synthesis. CYC1 also regulates apoptosis, or programmed cell death, by modulating the release of cytochrome c from mitochondria, which triggers the activation of caspases and leads to cell death.

  • 产品名称：
    Mouse anti-Homo sapiens (Human) CYC1 Monoclonal antibody
  • Uniprot No.：
    P08574
  • 基因名：
  • 别名：
    Cytochrome c1, heme protein, mitochondrial (EC:7.1.1.8); Complex III subunit 4; Complex III subunit IV; Cytochrome b-c1 complex subunit 4; Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit; Cytochrome c-1
  • 宿主：
    Mouse
  • 反应种属：
    Human, Mouse
  • 免疫原：
    Recombinant Human Cytochrome c1 protein (85-325AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    IgG1
  • 纯化方式：
    >95%, Protein A purified
  • 克隆号：
    27E12D11
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, FC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    FC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. Cytochrome c1 is a catalytic core subunit containing a c-type heme. It transfers electrons from the [2Fe-2S] iron-sulfur cluster of the Rieske protein to cytochrome c.
  • 基因功能参考文献：
    1. these results indicate that CYC1 plays important roles in cell proliferation and comedo necrosis through the elevated oxidative phosphorylation activity in human ductal carcinoma in situ PMID: 28394473
    2. results indicate that miR-661 plays a tumor suppressor role in OS mediated by the downregulation of CYC1, suggesting a potential mechanism underlying cell death resistance in Osteosarcoma. PMID: 28391262
    3. CYC1 promoted tumor metastasis via suppressing activation of AMPK and contributed to tumor growth via facilitating production of ATP. PMID: 27239088
    4. FASL, granzyme B, and cytochrome c blood expression reflects breast cancer progression and response to therapy. (Review) PMID: 27117663
    5. our results provide evidence that CYC1 plays an important role in OS tumorigenesis, and modulation of CYC1 may be an effective strategy to potentiate OS to apoptotic induction by TRAIL. PMID: 25562155
    6. Mutations in CYC1 cause insulin-responsive hyperglycemia. PMID: 23910460
    7. HeLa cells expressing non-cleavable cytochrome c1 maintain mitochondrial morphology and functions. PMID: 21577235
    8. Decreased electron Transport Complex IV activity is associated with ulcerative colitis. PMID: 20440543
    9. Addition of H202 to tendon fibroblasts in vitro resulted in oxidative stress-induced apoptosis and the release of CYC1. PMID: 12788227
    10. the Ca(2+) spike, mitochondrial Bcl-2 presensitization, and subsequent Delta psi(m) transition, Bax translocation, and ROS generation are important upstream signals for cytochrome c release upon stimulation by a Bcl-2 inhibitor. PMID: 14966123
    11. Sensitivity of Cyt c detection in -linked immunosorbent assay is reduced in the presence of serum leucine-rich alpha-2-glycoprotein-1. PMID: 16699948

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  • 相关疾病：
    Mitochondrial complex III deficiency, nuclear 6 (MC3DN6)
  • 亚细胞定位：
    Mitochondrion inner membrane; Single-pass membrane protein.
  • 蛋白家族：
    Cytochrome c family
  • 数据库链接：

    HGNC: 2579

    OMIM: 123980

    KEGG: hsa:1537

    STRING: 9606.ENSP00000317159

    UniGene: Hs.289271