Enterobacteria phage M13 III Monoclonal Antibody
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货号:CSB-MA303116A1m
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规格:¥1320
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图片:
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Western Blot
Positive WB detected in: 1-4 lanes: Recombinant Enterobacteria phage M13 Attachment protein at 2.5ng, 5ng, 10ng, 20ng; 5 lane: Enterobacteria phage M13 Attachment
All lanes: Enterobacteria phage M13 III antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 65, 55-70 KDa
Observed band size: 65, 55-70 KDa
Exposure time:3min
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其他:
产品详情
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产品描述:
CUSABIO generated hybridomas by fusing myeloma cells with B cells isolated from mice that were immunized with the recombinant Enterobacteria phage M13 attachment protein (19-424aa). After careful selection, CUSABIO obtained the Enterobacteria phage M13 III monoclonal antibody-secreting hybridomas, which were cultured to harvest the Enterobacteria phage M13 III monoclonal antibody. This antibody exhibits high specificity towards the Enterobacteria phage M13 species, making it ideal for use in ELISA and WB applications. It has been purified using protein G affinity chromatography, resulting in a purity of over 95%.
The Enterobacteria phage M13 Attachment protein (pIII) is a protein that functions as a virion protein for the M13 bacteriophage. It is responsible for attaching the phage to the host cell's pilus during the infection process. Specifically, the pIII protein binds to the F pilus on the surface of the bacterial host cell, which triggers the phage to insert its genome into the host cell. It plays a crucial role in the initial stages of the M13 phage infection process.
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产品名称:Mouse anti-Enterobacteria phage M13 III Monoclonal antibody
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Uniprot No.:P69168
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基因名:III
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宿主:Mouse
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反应种属:Enterobacteria phage M13
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免疫原:Recombinant Enterobacteria phage M13 Attachment protein (19-424AA)
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免疫原种属:Enterobacteria phage M13
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标记方式:Non-conjugated
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克隆类型:Monoclonal
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抗体亚型:IgG1
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纯化方式:>95%, Protein G purified
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克隆号:4B1D7
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浓度:It differs from different batches. Please contact us to confirm it.
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保存缓冲液:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
产品提供形式:Liquid
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应用范围:ELISA, WB
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推荐稀释比:
Application Recommended Dilution WB 1:1000-1:5000 -
Protocols:
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储存条件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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货期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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靶点详情
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功能:Plays essential roles both in the penetration of the viral genome into the bacterial host via pilus retraction and in the extrusion process. During the initial step of infection, G3P mediates adsorption of the phage to its primary receptor, the tip of host F-pilus. Subsequent interaction with the host entry receptor tolA induces penetration of the viral DNA into the host cytoplasm. In the extrusion process, G3P mediates the release of the membrane-anchored virion from the cell via its C-terminal domain.
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亚细胞定位:Virion. Host membrane; Single-pass type I membrane protein. Note=Prior to assembly, G3P is found associated with the bacterial host inner membrane. There are about five copies of this protein per mature phage that are located on the head side of the filamentous virion.
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蛋白家族:Inovirus G3P protein family
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数据库链接:
KEGG: vg:927334
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