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  4. MERTNL Monoclonal Antibody

MERTNL Monoclonal Antibody

  • 货号：
    CSB-MA731035A0m
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-MA731035A0m diluted at 1:50 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Overlay Peak curve showing Hela cells stained with CSB-MA731035A0m (red line) at 1:50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • 产品描述：

    The MERTNL monoclonal antibody is generated using the recombinant human MERTNL protein (46-311aa) as the immunogen to immunize mice. The B cells are isolated from the immunized mice and are fused with myeloma cells to produce hybridoma cells, which are screened to select the hybridoma cell line that secretes the MERTNL antibody. The MERTNL antibody-generating hybridoma cell line is injected into the mouse abdominal cavity. The MERTNL monoclonal antibody is purified and obtained from the mouse ascites using protein A affinity chromatography with a purity of over 95%. It is an unconjugated IgG2a antibody that recognizes human MERTNL protein and is suitable for use in ELISA, IHC and FC applications.

    MERTNL is a protein that acts as a ligand for the MER receptor tyrosine kinase, which is a member of the TAM family of receptor tyrosine kinases that play important roles in the regulation of immune responses, inflammation, and homeostasis. MERTNL is primarily expressed in the placenta and has been implicated in regulating the development and function of the placenta.

  • 产品名称：
    Mouse anti-Homo sapiens (Human) METRNL Monoclonal antibody
  • Uniprot No.：
    Q641Q3
  • 基因名：
    METRNL
  • 别名：
    Meteorin like glial cell differentiation regulator antibody; Meteorin; glial cell differentiation regulator like antibody; Meteorin-like protein antibody; METRL_HUMAN antibody; Metrnl antibody
  • 宿主：
    Mouse
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Meteorin-like protein (46-311AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    IgG2a
  • 纯化方式：
    >95%, Protein A purified
  • 克隆号：
    1F7A6
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, FC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Hormone induced following exercise or cold exposure that promotes energy expenditure. Induced either in the skeletal muscle after exercise or in adipose tissue following cold exposure and is present in the circulation. Able to stimulate energy expenditure associated with the browning of the white fat depots and improves glucose tolerance. Does not promote an increase in a thermogenic gene program via direct action on adipocytes, but acts by stimulating several immune cell subtypes to enter the adipose tissue and activate their prothermogenic actions. Stimulates an eosinophil-dependent increase in IL4 expression and promotes alternative activation of adipose tissue macrophages, which are required for the increased expression of the thermogenic and anti-inflammatory gene programs in fat. Required for some cold-induced thermogenic responses, suggesting a role in metabolic adaptations to cold temperatures.
  • 基因功能参考文献：
    1. downregulation of METRNL during adipogenesis and functional induction of increased proliferation in stromal vascular fraction cells with concomitant inhibition of adipocyte differentiation may result in hypertrophic AT accumulation PMID: 27748745
    2. Overexpression of METRNL inhibited mineralized nodule formation. PMID: 27716826
    3. Data suggest that there is no correlation between serum Metrnl levels and BMI (body mass index) in humans. PMID: 26307585
    4. Results show that Subfatin is a novel adipokine regulated by adipogenesis and obesity, with tissue distribution different from its homologue Meteorin PMID: 24393292
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Meteorin family
  • 组织特异性：
    Highly expressed in the skeletal muscle, in subcutaneous adipose tissue, epididymal white adipose tissue depots and heart. Also expressed in brown adipose tissues and kidney.
  • 数据库链接：

    HGNC: 27584

    OMIM: 616241

    KEGG: hsa:284207

    STRING: 9606.ENSP00000315731

    UniGene: Hs.378821