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Recombinant Human Melanoma-derived growth regulatory protein (MIA)

In Stock
  • 货号：
    CSB-AP000431HU
  • 规格：
    ￥852
  • 图片：
  • 其他：

产品详情

  • 纯度：
    >98% as determined by SDS-PAGE.
  • 内毒素：
    Less than 1.0 EU/μg as determined by LAL method.
  • 生物活性：
    Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human A375 cell line is less than 5 μg/ml, corresponding to a specific activity of >200 IU/mg.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cartilage derived retinoic acid sensitive protein; CD RAP; CDRAP; Melanoma derived growth regulatory protein precursor; Melanoma inhibitory activity protein; Melanoma-derived growth regulatory protein; Mia; MIA_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.Coli
  • 分子量：
    12.1 kDa
  • 表达区域：
    25-131aa
  • 氨基酸序列
    GPMPKLADRK LCADQECSHP ISMAVALQDY MAPDCRFLTI HRGQVVYVFS KLKGRGRLFW GGSVQGDYYG DLAARLGYFP SSIVREDQTL KPGKVDVKTD KWDFYCQ
  • 蛋白标签：
    Tag-Free
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    0.2 µm filtered concentrated solution in PBS, pH 7.4, with 5 % Trehalose,lyophilized
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    5-10 business days
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
  • 基因功能参考文献：
    1. the molecular basis of the interaction of MIA with the Hep II domain of fibronectin based on nuclear magnetic resonance spectroscopic binding assays. PMID: 28565914
    2. The frequency of MIA gene family expression was higher among squamous cell carcinomas than among other tumor types subjected to screening. MIA gene family staining was observed frequently in esophageal and lung cancers associated with nodal and/or distant metastasis. In cervical cancers, MIA and TANGO immunostaining also correlated with tumor progression and metastasis. PMID: 27145272
    3. Our results suggest that MIA-STOX2 signaling may be a useful diagnostic and therapeutic target in oral squamous cell carcinoma PMID: 27050375
    4. MIA had a slightly superior sensitivity to detect progressive disease compared to S100 and seems to be more useful in monitoring of patients with metastatic melanoma receiving immunotherapy PMID: 28870930
    5. real-time RT-PCR assays showed that expressions of MIA and MIA-RAB4B located 35 kb upstream of the deletion, were up-regulated in the polyps compared to the matched mucosa of the proband. MIA-RAB4B, the read-through long non-coding RNA (lncRNA), RAB4B, PIM2 and TAOK1 share common binding site of a microRNA, miR-24, in their 3'UTRs PMID: 28306719
    6. The effects of MIA/CD-RAP on transcriptional regulation in chondrocytes, through the regulation of p54(nrb) via YBX1 contributes to the understanding of chondrogenesis. PMID: 24349210
    7. data provide evidence for a critical role of SOX10 in melanoma cell invasion through the regulation of MIA and highlight its role as a therapeutic target in melanoma PMID: 24608986
    8. Focus on the quantitative analysis of the MIA protein as a prognostic tool because it has proven to be a useful serum marker for documenting disease progression of malignant melanoma. Review. PMID: 24372647
    9. Functional promoter analysis identified the transcription factor YBX1 as the mediator of MIA activation of p54(nrb) transcription. PMID: 23672612
    10. MIA protein is present in non-segmental vitiligo skin and may cause the detachment of melanocytes; its target is integrin alpha5beta1, which determines the breaking and/or weakening of connections among melanocytes and basal membrane PMID: 23664187
    11. Results show that S-100B, MIA and LDH levels were significantly higher in patients with advanced melanoma than in disease-free patients or healthy controls. PMID: 21858537
    12. assessed the utility of melanoma inhibitory activity (MIA) serum marker in the follow up and primary diagnosis of stage III melanoma patients PMID: 21658116
    13. Further diagnostics should be initiated in uveal melanoma patients with serum MIA above 8.3ng/ml. PMID: 21540751
    14. Data suggest that plasma markers: CEACAM, ICAM-1, osteopontin, MIA, TIMP-1 and S100B particularly when assessed in combination, can be used to monitor patients for disease recurrenc. PMID: 21487066
    15. The cell-specific production rate of MIA was quantitatively proportional to the aggrecan gene expression level in the early and middle phase of cartilage chondrocyte differentiation. PMID: 21277254
    16. MIA/CD-RAP stabilizes cartilage differentiation and inhibits differentiation into bone potentially by regulating signaling processes during differentiation. PMID: 20164682
    17. pancreatic cancer patients with high intratumoral expression are antibody-negative and have shorter survival PMID: 20514540
    18. A fluorescence polarization biological assay was developed using MIA protein-binding compounds for studies of the binding properties of this protein. PMID: 19852767
    19. The assignments, solution structure, & dynamics of human MIA were determined by heteronuclear NMR methods. The structure consists of an SH3-like subdomain with N- and C-terminal extensions of about 20 amino acids each that form a novel fold. PMID: 11991352
    20. Melanoma-inhibiting activity (MIA/CD-RAP) is expressed in a variety of malignant tumors of mainly neuroectodermal origin. PMID: 12014625
    21. expression pattern of a novel splice product MIA (splice) of malignant melanoma-derived growth-inhibiting activity (MIAY CD-RAP). PMID: 12230496
    22. Stable antisense-HMG1 expression in melanoma cells led to the reduction of melanoma inhibitory activity (MIA) promoter activity and protein expression. PMID: 12665595
    23. increased MIA production may, in turn, increase the invasive properties of the cells by modulating the attachment of human uveal melanoma cells to the extracellular matrix PMID: 15057037
    24. MIA may promote the detachment of radial and vertical growth phase melanomas. PMID: 15201995
    25. The MIA protein enhances the migration of melanocytes and promotes melanoma progression. PMID: 15208686
    26. MIA may contribute to immunosuppression frequently seen in malignant melanomas by inhibiting cellular antitumor immune reactions. PMID: 15386421
    27. MIA in homogenates of surgical specimen directly relate to a more benign clinical prognosis in patients with high-grade glioma PMID: 15547763
    28. There is a correlation between MIA expression and pigmentation and morphology of melanocytic cells. PMID: 15760338
    29. Increased levels of MIA is associated with gastric cancer PMID: 16331256
    30. When patients progressed, level of MIA increased significantly. PMID: 17348447
    31. A candidate autoantigen in rheumatoid arthritis found in synovial fluid cells. PMID: 17599744
    32. An antigen in melanoma, elevated in 22 per cent of patients, predicting recurrence. PMID: 17661202
    33. MIA serum level is the ideal test for screening the skin melanoma spread to sentinel lymph nodes. PMID: 18477894
    34. MIA expression is enhanced by the interaction of intracellular HMGB1 and NFkBp65 and MIA is closely involved in tumor progression and nodal metastasis by the increments of VEGF-C and VEGF-D in oral squamous cell carcinomas. PMID: 18616526
    35. MIA protein, binding to integrins and thus promoting detachment of cells from extracellular matrix structures, is internalized into the cell together with these cell adhesion receptors at the cell rear. PMID: 19521988

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    MIA/OTOR family
  • 组织特异性：
    All malignant melanoma cell lines tested and infrequently in glioma cell lines.
  • 数据库链接：

    HGNC: 7076

    OMIM: 601340

    KEGG: hsa:8190

    STRING: 9606.ENSP00000263369

    UniGene: Hs.646364