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ACBD3 Antibody

  • 货号：
    CSB-PA863972LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Rat kidney tissue
      All lanes: ACBD3 antibody at 7μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 61 kDa
      Observed band size: 61 kDa
    • IHC image of CSB-PA863972LA01HU diluted at 1:500 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA863972LA01HU diluted at 1:500 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated ABC system.
    • Immunofluorescence staining of Hela cells with CSB-PA863972LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) ACBD3 Polyclonal antibody
  • Uniprot No.：
    Q9H3P7
  • 基因名：
    ACBD3
  • 别名：
    ACBD 3 antibody; ACBD3 antibody; Acyl CoA binding domain containing protein 3 antibody; Acyl Coenzyme A binding domain containing 3 antibody; Acyl-CoA-binding domain-containing protein 3 antibody; GCP 60 antibody; GCP60 antibody; GCP60_HUMAN antibody; GOCAP 1 antibody; GOCAP1 antibody; Golgi complex associated protein 1 60kDa antibody; Golgi complex associated protein 1 antibody; Golgi complex-associated protein 1 antibody; Golgi phosphoprotein 1 antibody; Golgi resident protein GCP60 antibody; GOLPH 1 antibody; GOLPH1 antibody; PAP 7 antibody; PAP7 antibody; PBR and PKA associated protein 7 antibody; PBR associated protein antibody; PBR- and PKA-associated protein 7 antibody; Peripheral benzodiazepine receptor associated protein PAP7 antibody; Peripheral benzodiazepine receptor-associated protein PAP7 antibody; Peripherial benzodiazepine receptor associated protein antibody; PKA (RIalpha) associated protein antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Rat
  • 免疫原：
    Recombinant Human Golgi resident protein GCP60 protein (263-366AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，ACBD3 Antibody (CSB-PA863972LA01HU)，的标记方式是Non-conjugated。对于ACBD3 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA863972LB01HU ACBD3 Antibody, HRP conjugated ELISA
    FITC CSB-PA863972LC01HU ACBD3 Antibody, FITC conjugated
    Biotin CSB-PA863972LD01HU ACBD3 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:300-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Involved in the maintenance of Golgi structure by interacting with giantin, affecting protein transport between the endoplasmic reticulum and Golgi. Involved in hormone-induced steroid biosynthesis in testicular Leydig cells. Recruits PI4KB to the Golgi apparatus membrane; enhances the enzyme activity of PI4KB activity via its membrane recruitment thereby increasing the local concentration of the substrate in the vicinity of the kinase.; (Microbial infection) Plays an essential role in Aichi virus RNA replication by recruiting PI4KB at the viral replication sites.
  • 基因功能参考文献：
    1. These results reveal a mechanism of EV71 replication that involves host ACBD3 for viral replication. PMID: 28303920
    2. that ACBD3 may represent candidate therapeutic targets to enable the elimination of breast cancer stem cells PMID: 29307786
    3. ACBD3 was found as a gene expression variability marker in 8-cell human embryos. PMID: 26288249
    4. the component proteins of the machinery, OSBP, VAP, SAC1, and PITPNB, are all essential host factors for AiV replication. Importantly, the machinery is directly recruited to the RNA replication sites through previously unknown interactions of VAP/OSBP/SAC1 with the AiV proteins and with ACBD3. PMID: 29367253
    5. Results show that Aichi virus 3A protein activates the lipid kinase activity of PI4KIIIb,which activation is sensitized by the protein ACBD3. The interfaces between PI4KIIIbeta-ACBD3 and ACBD3-3A were mapped with hydrogen-deuterium exchange mass spectrometry. PMID: 27989622
    6. Kobuviral non-structural 3A proteins act as molecular harnesses to hijack the host ACBD3 protein. PMID: 28065508
    7. The s propose a model in which interaction between Salmonella enterica serovar Typhimurium SseF and SseG enables both proteins to bind ACBD3, thereby anchoring Salmonella-containing vacuoles at the Golgi network and facilitating bacterial replication. PMID: 27406559
    8. these findings suggest that ACBD3 has prominent impacts on cellular NAD(+) metabolism via regulating PARP1 activation-dependent auto-modification and thus cell metabolism and function. PMID: 25940138
    9. s found that NS5A and PI4KB competed for association of acyl-coenzyme A binding domain containing protein 3 (ACBD3), which inhibited hepatitis C virus replication. PMID: 24792752
    10. Host ACBD3, PI4KIIIBETA and Aichi virus proteins complexes enhances phosphatidylinositol 4-phosphate synthesis and is critical for viral replication. PMID: 24672044
    11. ACBD3 mediates mHtt cytotoxicity via a Rhes/mHtt/ACBD3 complex. PMID: 24012756
    12. s found that the Golgi protein acyl-coenzyme A binding domain-containing 3 (ACBD3), interacts with the 3A proteins of poliovaccine Sabintpe 2 and coxsackievirus A17 at viral RNA replication sites. PMID: 23926333
    13. ACBD3 plays an essential role in maintaining lipid homeostasis via regulating SREBP1's processing pathway and thus impacting cellular lipogenesis. PMID: 23166793
    14. identified ACBD3 as an interacting partner of PPM1L, and showed that this association, which recruits PPM1L to ER-Golgi membrane contact sites, is mediated by a GOLD (Golgi dynamics) domain in ACBD3 PMID: 22796112
    15. The data were consistent with PAP7 interacting with DMT1 and regulating DMT1 expression in K562 cells by modulating expression of DMT1 protein. PMID: 22383495
    16. The 3A protein of picornaviruses utilizes the golgi adaptor protein ACBD3 to recruit PI4KIIIbeta. PMID: 22258260
    17. results indicate that a viral protein/ACBD3/PI4KB complex is formed to synthesize PI4P at the AiV RNA replication sites and plays an essential role in viral RNA replication PMID: 22124328
    18. Molecular cloning, chromosomal localization and expression pattern of PAP7. PMID: 12692076
    19. GCP60 interacts preferentially with one of the golgin-160 caspase cleavage fragments (residues 140-311). This strong interaction prevented the golgin-160 fragment from accumulating in the nucleus when this fragment and GCP60 were overexpressed. PMID: 16870622
    20. nuclear translocation of golgin-160-(140-311) is a highly coordinated event regulated not only by cleavage of the golgin-160 head but also by the oxidation state of GCP60 PMID: 17711851

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  • 亚细胞定位：
    Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion.
  • 组织特异性：
    Ubiquitous, with highest expression in testis and ovary.
  • 数据库链接：

    HGNC: 15453

    OMIM: 606809

    KEGG: hsa:64746

    STRING: 9606.ENSP00000355777

    UniGene: Hs.520207