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  4. AGAP2 Antibody

AGAP2 Antibody

  • 货号：
    CSB-PA014480
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA014480(AGAP2 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)
    • The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using CSB-PA014480(AGAP2 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)
    • Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: Hela cells, Primary antibody: CSB-PA014480(AGAP2 Antibody) at dilution 1/700, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds
  • 其他：

产品详情

  • Uniprot No.：
    Q99490
  • 基因名：
  • 别名：
    AGAP-2 antibody; Agap2 antibody; Agap2 ArfGAP with GTPase domain; ankyrin repeat and PH domain 2 antibody; AGAP2_HUMAN antibody; ANK repeat and PH domains 2 antibody; Arf GAP with GTP binding protein like antibody; Arf GAP with GTP-binding protein-like; ANK repeat and PH domains 2 antibody; Arf GAP with GTP-binding protein-like; ankyrin repeat and pleckstrin homology domains 2 antibody; Arf-GAP with GTPase; ANK repeat and PH domain-containing protein 2 antibody; ArfGAP with GTPase domain; ankyrin repeat and PH domain 2 antibody; Centaurin gamma 1 antibody; centaurin gamma1 antibody; Centaurin-gamma-1 antibody; Centg1 antibody; cnt g1 antibody; Cnt-g1 antibody; Cnt2 antibody; FLJ16430 antibody; GGAP2 antibody; GTP binding and GTPase activating protein 2 antibody; GTP-binding and GTPase activating protein 2 antibody; GTP-binding and GTPase-activating protein 2 antibody; KIAA0167 antibody; mKIAA0167 antibody; Nuclear GTPase PIKE antibody; Phosphatidylinositol 3-kinase enhancer antibody; Phosphoinositide 3 kinase enhancer antibody; PI3K ENHANCER antibody; PIKE antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Synthetic peptide of Human AGAP2
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:2000-1:5000
    WB 1:500-1:2000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion.
  • 基因功能参考文献：
    1. A pleckstrin homology domain in PIKE-L directly binds alpha-synuclein and antagonizes its aggregation. Accordingly, PIKE-L overexpression decreases dopaminergic cell death elicited by MPP(+), whereas PIKE-L knockdown elevates alpha-synuclein oligomerization and cell death. PMID: 28096359
    2. these findings support that PIKE amplification or overexpression coordinately acts with CDK4 to drive glioblastoma tumorigenesis. PMID: 28368413
    3. Studies showed that overexpression or mutation of PIKE has been observed in a variety of tumors, promoting cancer cell growth, transformation and invasion through AKT signaling or other signaling pathways, such as focal adhesion kinase. [review] PMID: 26977005
    4. Mutation of Fyn phosphorylation sites on PIKE-A, depletion of Fyn, or pharmacological inhibition of Fyn blunts the association between PIKE-A and AMPK, resulting in loss of its inhibitory effect on AMPK. PMID: 26001218
    5. expression of PLC-gamma1 and PIKE positively correlated with the tumor differentiation of oral squamous cell carcinoma. PMID: 26464646
    6. PIKE reduction rescued PI3K-dependent and -independent neuronal defects in fragile X syndrome. PMID: 25921541
    7. In this review the CENTG1 gene is amplified in a variety of human cancers which lead to the enhancement of tumor invasion. PMID: 23770988
    8. AGAP2 plays a role in the signalling and recycling of beta2-adrenergic receptors. PMID: 23527545
    9. Fyn regulates the activity of the adipogenic transcription factor signal transducer and activator of transcription 5a (STAT5a) through enhancing its interaction with the GTPase phosphoinositide 3-kinase enhancer A (PIKE-A). PMID: 23438599
    10. PIKE is highly expressed in human squamous cell carcinoma and has a critical role in EGF-induced squamous cell carcinoma proliferation. PMID: 22349826
    11. The presence of heterogeneous missense mutations of GGAP2 in prostate cancer was associated with aggressive clinical behavior. PMID: 22389719
    12. PIKE is a critical factor in controlling synaptic AMPA receptor insertion. PMID: 21847098
    13. Akt-phosphorylated PIKE-A inhibits UNC5B-induced apoptosis in cancer cell lines in a p53-dependent manner. [PIKE-A] PMID: 21460185
    14. regulates retrograde protein transport between early endosomes and the Golgi complex PMID: 20551179
    15. This study have identified, cloned and characterized PIKE-L, which localizes to both the cytoplasm and the nucleus, activates mGlur1 enhances formation of an mGluRI-Homer-PIKE-L complex, then activates PI3 kinase activity and prevents neuronal apoptosis. PMID: 14528310
    16. a physiologic regulator of Akt and an oncogenic effector of cell invasion PMID: 14761976
    17. PIKE binds PI 3-kinase & stimulates its lipid kinase activity. There are 3 isoforms which function throught the cell and meadiate processes from invasiveness to apoptosis. Review. PMID: 15951849
    18. The AGAP2 colocalized with AP-1, transferrin receptor and Rab4 on endosomes. Overexpression of AGAP2 changed the intracellular distribution of AP-1 and promoted Rab4-dependent fast recycling of transferrin. PMID: 16079295
    19. an important role of PIKE gene aberrations in the molecular pathogenesis of primary glioblastomas PMID: 16150119
    20. Fyn is essential for phosphorylating PIKE-A and protects it from apoptotic cleavage. PMID: 16841086
    21. The results of this study indicate that centaurins could be more accurately classified as NTPases and point to alternative mechanisms of cell signalling control. PMID: 17037982
    22. Myxoma virus (MV) M-T5 host range protein is functionally interchangeable with the host PIKE-A protein and that the activation of host Akt by either M-T5 or PIKE-A is critical for the permissiveness of cancer cells for MV. PMID: 17151107
    23. PIKE-A acts as a proto-oncogene, promoting cell transformation through Akt activation. PMID: 17297440
    24. Ectopic expression of AGAP2 causes both BRAG2 and the coiled bodies' marker coilin to accumulate in nucleoli. PMID: 17461797
    25. The cytoplasmic-nuclear shuttling of PIKE is dynamically regulated by the balancing actions of the lipid-binding property of both the split PH domain and the nuclear targeting function of its nuclear localization sequence. PMID: 18371979
    26. In normal mice. PIKE-L strongly binds SET and prevents its degradation by AEP, leading to resistance of neuronal cell death. PMID: 18374643
    27. PIKE-L acts as a downstream survival effector for netrin-1 through UNC5B in the nervous system PMID: 18469807
    28. Cdk5 phosphorylates PIKE-A and stimulates its GTPase activity, which activates nuclear Akt and promotes glioblastoma cell migration and invasion. PMID: 18487454
    29. Increased GGAP2 expression, which is present in three quarters of human prostate cancers, can activate two critical pathways that have been linked to prostate cancer initiation and progression. PMID: 19176382
    30. AGAP2 regulates the FAK activity and the focal adhesion disassembly during cell migration. PMID: 19318351

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  • 亚细胞定位：
    [Isoform 1]: Cytoplasm. Nucleus.; [Isoform 2]: Cytoplasm.
  • 蛋白家族：
    Centaurin gamma-like family
  • 组织特异性：
    Isoform 1 is brain-specific. Isoform 2 is ubiquitously expressed, with highest levels in brain and heart.
  • 数据库链接：

    HGNC: 16921

    OMIM: 605476

    KEGG: hsa:116986

    STRING: 9606.ENSP00000449241

    UniGene: Hs.302435