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AGO4 Antibody

  • 货号：
    CSB-PA935743XA01DOA
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: recombinant protein
      All lanes: AGO4 Antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 33 kDa
      Observed band size: 33 kDa
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AGO4 Polyclonal antibody
  • Uniprot No.：
    Q9ZVD5
  • 基因名：
  • 别名：
    Protein argonaute 4 (Protein OVEREXPRESSOR OF CATIONIC PEROXIDASE 11) AGO4 OCP11 At2g27040 T20P8.9
  • 宿主：
    Rabbit
  • 反应种属：
    Arabidopsis thaliana
  • 免疫原：
    Recombinant Arabidopsis thaliana AGO4 protein (101-360aa)
  • 免疫原种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Together with RDM3, required for transcriptional gene silencing (TGS) by DNA methylation and repressive histone modifications (H3K9me2) of several chromatin loci. Component of the RISC complex that associate with the small interfering RNA (siRNA) pathway involved in direct cytosine methylation at endogenous DNA repeats. Forms a AGO4/NRPE1/siRNA complex in cajal body, facilitating its function in RNA-directed gene silencing of target loci. Required for CpNpG and asymmetric DNA methylation as well as histone H3 'Lys-9' methylation (H3K9me) at SUP and SN1 loci. May be not required for CpG methylation. Required for the production and maintenance of retrotransposon SN1 and Copia and ribosomal 5S 25 nucleotide siRNAs specialized in gene silencing at chromatin level. Involved in de novo methylation of FWA gene and required for the maintenance of RNA-directed DNA methylation (RdDM) triggered by inverted repeat transgenes. Interacts with miRNA miR390 and miR172, targeting respectively TAS3 and AP2 mRNAs, and mediates cleavage of miRNA targets. Associates mainly with small RNAs of 24 nucleotide in length and preferentially recruits small RNAs with a 5' terminal adenosine. Targeted by the turnip yellows virus (TuYV) protein P0 (via F-box-like domain) for probable proteasome degradation and thereby inactivating AGO4 function in RNA silencing. Required for resistance to the bacterial pathogen P.syringae. Works independently of the RdDM pathway in mediating resistance to P.syringae. RdDM is involved in viral genome methylation as an epigenetic defense against geminiviruses.
  • 基因功能参考文献：
    1. DDB2 forms a complex with ROS1 and AGO4 and that they act at the ROS1 locus to modulate levels of DNA methylation and therefore ROS1 expression. DDB2 acts as a critical regulator of ROS1-mediated active DNA demethylation. PMID: 29078035
    2. These results indicate an important role for AGO4 in targeting plant RNA viruses as well as demonstrating novel mechanisms of regulation of and by AGO4, independent of its canonical role in regulating gene expression by RNA-dependent DNA methylation (RdDM). PMID: 27762650
    3. Data suggest that AGO4-dependent siRNAs are secondary siRNAs dependent on the prior activity of the RdDM pathway at certain loci. PMID: 28002617
    4. demonstrate that Pol V lncRNAs or the act of their transcription are required to lock Pol V holoenzyme into a stable DNA-bound state that allows AGO4 recruitment via redundant glycine-tryptophan/tryptophan-glycine AGO hook motifs present on both Pol V and its associated factor, SPT5L. PMID: 27986858
    5. Findings reported here highlight an involvement of AGO4 during meiosis by ensuring accurate chromosome segregation at anaphase I. PMID: 27466226
    6. Members of the AGO4 clade cooperatively participate in preventing the abnormal specification of multiple premeiotic gametophytic precursors during early ovule development. PMID: 27591749
    7. Our results indicate that AtMBD6 is involved in RNA-mediated gene silencing and it binds to RNA binding proteins like AtRPS2C, AtAGO4 and AtNTF2. AtMBD6 also interacts with histone deacetylase AtHDA6 that might have a role in chromatin condensation at the targets of RdDM PMID: 28229965
    8. These findings suggest that the targets of AGO4-directed RNA-directed DNA methylation are regulatory units responsible for controlling gene expression under specific environmental conditions. PMID: 23013441
    9. AGO4 and AGO6 mainly act sequentially in mediating small RNA-directed DNA methylation. PMID: 25527293
    10. Trans-acting small interfering RNAs are associated with AGO4 proteins to direct DNA methylation. PMID: 22846193
    11. selective nuclear import of mature AGO4/siRNA complexes is a key regulatory point prior to the effector stage of RdDM. PMID: 22608924
    12. DNA-dependent RNA polymerase IV-dependent smRNAs were mainly 24 nt and associated with AGO4. PMID: 21457371
    13. AGO4 is guided to target loci through base-pairing of associated siRNAs with nascent Pol V transcripts. [AGO4] [ARGONAUTE4] PMID: 19377477
    14. Our results suggest a function for Cajal bodies as a center for the assembly of an AGO4/NRPD1b/siRNA complex, facilitating its function in RNA-directed gene silencing at target loci. PMID: 16839879
    15. AGO4 binds to small RNAs including small interfering RNAs (siRNAs) originating from transposable and repetitive elements, and cleaves target RNA transcripts PMID: 16998468
    16. AGO4 was partially compromised in the reception of silencing. PMID: 17785412
    17. OCP11 was cloned and shown that it encodes ARGONAUTE4 (AGO4), a component of the pathway that mediates the transcriptional gene silencing associated with small interfering RNAs that direct DNA methylation at specific loci. PMID: 17993621
    18. two classes of AGO4 bodies are structurally independent--disruption of one class does not affect the other--suggesting a dynamic regulation of AGO4 within two distinct nuclear compartments PMID: 18266474
    19. SPT5-like emerged in plants as a facultative RNA polymerase elongation factor. PMID: 19343051
    20. our data suggest that AGO4 is guided to target loci through base-pairing of associated siRNAs with nascent RNA Pol V transcripts. PMID: 19377477
    21. The dual interaction of an effector protein with AGO and small RNA target transcripts may be a general feature of RNA-silencing effector complexes PMID: 19410546

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  • 亚细胞定位：
    Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus, Cajal body.
  • 蛋白家族：
    Argonaute family, Ago subfamily
  • 组织特异性：
    Expressed in embryos, mature leaves, vascular tissue of the sepals, stamens and stigma, at the tip of the style and siliques.
  • 数据库链接：

    KEGG: ath:AT2G27040

    STRING: 3702.AT2G27040.1

    UniGene: At.13165