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AKAP8 Antibody

  • 货号:
    CSB-PA943275
  • 规格:
    ¥1100
  • 图片:
    • The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA943275(AKAP8 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)
    • Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane: Human liver cancer tissue, Primary antibody: CSB-PA943275(AKAP8 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
  • 其他:

产品详情

  • Uniprot No.:
    O43823
  • 基因名:
  • 别名:
    A kinase (PRKA) anchor protein 8 antibody; A kinase anchor protein 8 antibody; A kinase anchor protein 95 antibody; A kinase anchor protein 95 kDa antibody; A kinase anchor protein 95kDa antibody; A-kinase anchor protein 8 antibody; A-kinase anchor protein 95 kDa antibody; AKAP 8 antibody; AKAP 95 antibody; AKAP-8 antibody; AKAP-95 antibody; Akap8 antibody; AKAP8_HUMAN antibody; AKAP95 antibody; DKFZp586B1222 antibody
  • 宿主:
    Rabbit
  • 反应种属:
    Human
  • 免疫原:
    Synthetic peptide of Human AKAP8
  • 免疫原种属:
    Homo sapiens (Human)
  • 标记方式:
    Non-conjugated
  • 抗体亚型:
    IgG
  • 纯化方式:
    Antigen affinity purification
  • 浓度:
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液:
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式:
    Liquid
  • 应用范围:
    ELISA,WB,IHC
  • 推荐稀释比:
    Application Recommended Dilution
    ELISA 1:1000-1:2000
    WB 1:200-1:1000
    IHC 1:25-1:100
  • Protocols:
  • 储存条件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能:
    Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II). Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation; the function appears to be independent from PKA-anchoring. May help to deliver cyclin D/E to CDK4 to facilitate cell cycle progression. Required for cell cycle G2/M transition and histone deacetylation during mitosis. I...显示更多
  • 基因功能参考文献:
    1. These results establish AKAP95 as a mostly positive regulator of pre-mRNA splicing and a possible integrator of transcription and splicing regulation. PMID: 27824034
    2. Results suggest that ALK generated by alternative transcription Initiation induces chromatin structural changes and heterochromatinization through phosphorylation of AKAP8 in the nucleus. PMID: 29093346
    3. Using a BioID proximity-based proteomic screen, we identify the nuclear pore complex protein TPR as a novel AKAP95 binding partner. We show interaction between AKAP95 and TPR in mitosis, and an AKAP95-dependent enrichment of TPR in the spindle microtubule area in metaphase, then later in the spindle midzone area. PMID: 28379780
    4. Report expression of connexin 43 in ovarian cancer cells in G1/S phase. PMID: 26823747
    5. The data indicate that AKAP95 is a novel nucleolus-associated protein with a regulatory role on rRNA production. PMID: 26683827
    6. Gene dosage at 19p13.12, and AKAP8 and/or AKAP8L in particular, play an important role in modulation of head size and may contribute to autism risk. PMID: 26076356
    7. These results suggest that AKAP8 is involved in the regulation of chromatin structural changes through nuclear tyrosine phosphorylation. PMID: 25770215
    8. Ectopic AKAP95 stimulates expression of a chromosomal reporter gene in synergy with MLL1 or MLL2, whereas AKAP95 depletion impairs retinoic acid-mediated gene induction in embryonic stem cells PMID: 23995757
    9. AMY-1 interacts with S-AKAP84 and this protein in the cytoplasm and the nucleus, respectively, and inhibits cAMP-dependent protein kinase activity by preventing binding of its catalytic subunit to A-kinase-anchoring protein (AKAP) complex. PMID: 12414807
    10. role of AKAP95 in DNA replication by providing a scaffold for MCM2 PMID: 12740381
    11. AKAP95 is a potential carrier protein for active caspase 3 from the cytoplasm into the nuclei in apoptotic cells. PMID: 16227597

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  • 亚细胞定位:
    Nucleus. Nucleus matrix. Nucleus, nucleolus. Cytoplasm.
  • 蛋白家族:
    AKAP95 family
  • 组织特异性:
    Highly expressed in heart, liver, skeletal muscle, kidney and pancreas. Expressed in mature dendritic cells.
  • 数据库链接:

    HGNC: 378

    OMIM: 604692

    KEGG: hsa:10270

    STRING: 9606.ENSP00000269701

    UniGene: Hs.594496