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ALKBH2 Antibody

  • 货号：
    CSB-PA259131
  • 规格：
    ￥1100
  • 图片：
    • Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Human fetal liver tissue, Human seminoma tissue, RAW264.7 cells, Primary antibody: CSB-PA259131(ALKBH2 Antibody) at dilution 1/650, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
  • 其他：

产品详情

  • Uniprot No.：
    Q6NS38
  • 基因名：
    ALKBH2
  • 别名：
    2OG Fe(II) oxy DC1 antibody; 9530023G02 antibody; ABH 2 antibody; ABH2 antibody; alkB alkylation repair homolog 2 antibody; ALKB2_HUMAN antibody; ALKBH2 alkB antibody; ALKBH2 antibody; Alkylated DNA repair protein alkB homolog antibody; Alkylated DNA repair protein alkB homolog 2 antibody; Alkylation repair homolog 2 antibody; Alpha ketoglutarate dependent dioxygenase alkB homolog 2 antibody; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2 antibody; AU016977 antibody; DNA oxidative demethylase ALKBH2 antibody; EC 1.14.11. antibody; FLJ99103 antibody; hABH2 antibody; mABH2 antibody; MGC90512 antibody; Oxy DC1 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Fusion protein of Human ALKBH2
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:2000-1:5000
    WB 1:500-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.
  • 基因功能参考文献：
    1. These results revealed that N3-ethylthymidine , but not other DNA lesions, could be repaired by Alkbh2 and Alkbh3 in mammalian cells. PMID: 26930515
    2. studies reveal the ALKBH2 binding interface of PCNA and indicate that both germline and somatic ALKBH2 variants could have cellular effects on ALKBH2 function in DNA repair. PMID: 26408825
    3. It was shown for first time that DNA glycosylase ALKBH2 can repair DNA adduct 1,N2-ethenoguanine. PMID: 25797601
    4. ABH2 knockdown impairs rDNA transcription and leads to increased single-stranded and double-stranded DNA breaks in the rDNA genes. PMID: 23972994
    5. Overexpression of ALKBH2 is associated with enhanced resistance to temozolomide in glioblastoma. PMID: 23258843
    6. ALKBH2 is an upstream molecule of the oncoprotein, MUC1, and regulates cell cycle and EMT, resulting in progression of urothelial carcinomas. PMID: 23279696
    7. combination of duplex interrogation and oxidation chemistry allows ALKBH2 to detect and process diverse lesions efficiently and correctly PMID: 22659876
    8. The non-enzymatic binding of AAG to 3,N(4)-ethenocytosine specifically blocks ALKBH2-catalyzed repair of 3,N(4)-ethenocytosine but not that of methylated ALKBH2 substrates. PMID: 22079122
    9. X-ray absorption spectroscopy structural investigation of early intermediates in the mechanism of DNA repair by human ABH2 PMID: 21510633
    10. ABH2 is downregulated in a subset of gastric cancers, and might be involved in the molecular mechanism of gastric cancer through inhibiting the proliferation of gastric cancer cells. PMID: 21155885
    11. This work has provided a detailed understanding of the structural features of the single-stranded DNA and double-stranded DNA preferences of ABH2 and ABH3. PMID: 20714506
    12. Divergent sequences outside of the active site determine substrate specificities of ABH2. PMID: 20525795
    13. Tumour protein (TP53) is directly involved by binding to the promoter of ALKBH2 in mediating photofrin-mediaated photodynamic therapy in U87 glioma cells. PMID: 20661249
    14. first crystal structure of ABH2 PMID: 18432238
    15. ABH2 is active in the direct reversal of epsilon A lesions, and that ABH2, together with the alkyl-N-adenine-DNA glycosylase, which is the most effective enzyme for the repair of epsilon A, comprise the cellular defense against epsilon A lesions PMID: 18519673
    16. Mutations of ABH2 is associated with pediatric brain tumors. PMID: 19290481

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  • 亚细胞定位：
    Nucleus. Note=Detected in replication foci during S-phase.
  • 蛋白家族：
    AlkB family
  • 组织特异性：
    Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder.
  • 数据库链接：

    HGNC: 32487

    OMIM: 610602

    KEGG: hsa:121642

    STRING: 9606.ENSP00000343021

    UniGene: Hs.374458