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ALYREF Antibody

  • 货号：
    CSB-PA291999
  • 规格：
    ￥2024
  • 图片：
    • Western blot analysis of extracts from LOVO cells and K562 cells, using THOC4 antibody.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) ALYREF Polyclonal antibody
  • Uniprot No.：
    Q86V81
  • 基因名：
    ALYREF
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Synthesized peptide derived from internal of Human THOC4.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:3000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Export adapter involved in nuclear export of spliced and unspliced mRNA. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NFX1 pathway). Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm. TREX recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production; ALYREF/THOC4 mediates the recruitment of the TREX complex to the intronless viral mRNA. Required for TREX complex assembly and for linking DDX39B to the cap-binding complex (CBC). In conjunction with THOC5 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in the nuclear export of intronless mRNA; proposed to be recruited to intronless mRNA by ATP-bound DDX39B. Involved in transcription elongation and genome stability. Involved in mRNA export of C5-methylcytosine (m5C)-containing mRNAs: specifically recognizes and binds m5C mRNAs and mediates their nucleo-cytoplasmic shuttling.; Acts as chaperone and promotes the dimerization of transcription factors containing basic leucine zipper (bZIP) domains and thereby promotes transcriptional activation.
  • 基因功能参考文献：
    1. Dysregulation of ALYREF-mediated mRNA export upon NSUN2 depletion could be restored by reconstitution of wild-type but not methyltransferase-defective NSUN2. PMID: 28418038
    2. ALYREF mainly binds to the 5' and the 3' regions of the mRNA. ALYREF is likely recruited to mRNAs by PABPN1 and the 3' processing machinery. PMID: 28934468
    3. The competition between hMTR4 and ALYREF determines exosome recruitment and functions in creating balanced nuclear RNA pools for degradation and export. PMID: 28801509
    4. small hairpin RNA-mediated down-regulation of TAP or Aly reduced nuclear export of HDAg-L and assembly of HDV virions. Furthermore, a peptide, TAT-HDAg-L(198-210), containing the 10-amino acid TAT peptide and HDAg-L(198-210), inhibited the interaction between HDAg-L and TAP and blocked HDV virion assembly and secretion. PMID: 27807029
    5. A short conserved motif in ALYREF directs cap- and exon junction complex-dependent assembly of export complexes on spliced mRNAs. PMID: 26773052
    6. the data provides the first site-specific description of how viral mRNA is locked by a herpes viral adaptor protein in complex with cellular ALYREF, giving herpesvirus access to the cellular mRNA export machinery PMID: 24550725
    7. a spatial map is produced in living cells of the sites for the interaction of two TREX subunits, Alyref and Chtop, with Nxf1. PMID: 23826332
    8. ALY is a novel E2F2-interacting protein and a relevant modulator of E2F-responsive gene expression. PMID: 23297349
    9. The interaction of the cellular export adaptor protein Aly/REF with ICP27 contributes to the efficiency of herpes simplex virus 1 mRNA export. PMID: 23637401
    10. High ALY expression is associated with lymph node metastasis in oral squamous cell carcinoma. PMID: 23242234
    11. s propose that Aly, THO and UAP56 form a highly integrated unit to associate with the spliced mRNA and function in mRNA export. PMID: 23222130
    12. REF/Aly-ORF57 interaction is not essential for KSHV lytic replication but may contribute to target RNA stability independent of effects on RNA export, suggesting a novel role for REF/Aly in viral RNA metabolism. PMID: 22761374
    13. When the TREX components UAP56 or Aly are knocked down, spliced mRNA, as well as total polyA+ RNA, accumulates in nuclear speckle domains. PMID: 20981025
    14. Results describe the subcellular localization of ICP27 and its colocalization with cellular RNA export factors Aly/REF and TAP/NXF1. PMID: 20015986
    15. suggest a central role for ALY in T-cell enhancer function and oncogene activation PMID: 17229714
    16. Aly is a physiological target of nuclear PI3K signaling, which regulates its subnuclear residency, cell proliferation, and mRNA export activities through nuclear Akt phosphorylation and phosphoinositide association. PMID: 18562279
    17. Adaptor Aly functions in the Tap-p15-mediated nuclear export of HSP70 mRNA. PMID: 19165146
    18. The s demonstrate that ORF57 recruits several members of hTREX, namely Aly, UAP56 and hTHO-complex proteins, onto the viral mRNAs to assemble an export-competent ribonucleoprotein particle. PMID: 19264631
    19. TAP/NXF1, but not Aly/REF, is required for RNA export during HSV-1 infection. PMID: 19369354
    20. These results indicate that ICP27 RGG box methylation regulates interaction ALY and SRPK1 proteins. PMID: 19553338

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  • 亚细胞定位：
    Nucleus. Nucleus speckle. Cytoplasm.
  • 蛋白家族：
    ALYREF family
  • 组织特异性：
    Expressed in a wide variety of cancer types.
  • 数据库链接：

    HGNC: 19071

    OMIM: 604171

    KEGG: hsa:10189

    STRING: 9606.ENSP00000421592

    UniGene: Hs.534385