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  4. APLF (Ab-116) Antibody

APLF (Ab-116) Antibody

  • 货号：
    CSB-PA109959
  • 规格：
    ￥2024
  • 图片：
    • Immunohistochemistry analysis of paraffin-embedded human brain tissue using APLF (Ab-116) antibody.
    • Western blot analysis of extracts from HT-29 cells, using APLF (Ab-116) antibody.
    • Western blot analysis of extracts from COS7 cells (Lane 2), using APLF (Ab-116) antiobdy. The lane on the left is treated with systhesized peptide.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) APLF Polyclonal antibody
  • Uniprot No.：
    Q8IW19
  • 基因名：
    APLF
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthesized non-phosphopeptide derived from Human APLF around the phosphorylation site of serine 116 (R-N-S(p)-Q-V).
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:3000
    IHC 1:50-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage. Together with PARP3, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ).
  • 基因功能参考文献：
    1. characterization of the interaction of the APLF FHA domain with phosphorylated XRCC1 peptides PMID: 29059378
    2. These data suggest functional requirements for Ku and XRCC4 in APLF-dependent NHEJ and a unique role for Ku as a factor required to facilitate the nuclear retention of APLF. PMID: 23689425
    3. APLF promotes the assembly and activity of multi-protein Ku-DNA complexes containing all of the Non-homologous end joining (NHEJ) factors required for DNA ligation. PMID: 23178593
    4. Polynucleotide kinase and aprataxin-like forkhead-associated protein (PALF) acts as both a single-stranded DNA endonuclease and a single-stranded DNA 3' exonuclease and can participate in DNA end joining in a biochemical system. PMID: 21885877
    5. only a positive association (OR=1.58, 95% CI 1.05-2.46, P=0.027) was obtained for XRCC1 (Arg280His PMID: 21414327
    6. APLF has a role in chromosomal DNA double-strand break repair. PMID: 21211721
    7. The poly(ADP-ribose)-regulated protein APLF as a DNA-damage-specific histone chaperone. PMID: 21211722
    8. The s present solution structures of the two poly(ADP-ribose)-binding zinc finger modules of aprataxin and PNK-like factor (APLF), revealing a novel type of zinc finger. PMID: 20098424
    9. These data identify APLF as a novel component of the cellular response to DNA strand breaks in human cells. PMID: 17353262
    10. PALF is novel human AP endonuclease with conserved zinc-finger-like motifs involved in DNA strand break responses. PMID: 17396150
    11. APLF is an ATM target that is involved in nonhomologous end-joining and facilitates double-strand break repair, likely via interactions with Ku and XRCC4-DNA ligase IV. PMID: 18077224
    12. Study concludes that APLF can accumulate at sites of chromosomal damage via zinc finger-mediated binding to poly(ADP-ribose) and is a novel component of poly(ADP-ribose) signaling in mammalian cells. PMID: 18474613

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  • 亚细胞定位：
    Nucleus. Chromosome. Cytoplasm, cytosol.
  • 蛋白家族：
    APLF family
  • 数据库链接：

    HGNC: 28724

    OMIM: 611035

    KEGG: hsa:200558

    STRING: 9606.ENSP00000307004

    UniGene: Hs.720369