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APOBEC3F Antibody

  • 货号：
    CSB-PA816878LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Mouse heart tissue
      All lanes: APOBEC3F antibody at 2.7μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 46, 10, 12 kDa
      Observed band size: 46 kDa
    • Immunofluorescent analysis of HepG2 cells using CSB-PA816878LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) APOBEC3F Polyclonal antibody
  • Uniprot No.：
    Q8IUX4
  • 基因名：
    APOBEC3F
  • 别名：
    A3F antibody; ABC3F_HUMAN antibody; APOBEC3F antibody; Apolipoprotein B mRNA editing enzyme; catalytic polypeptide like 3F antibody; Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F antibody; ARP8 antibody; DNA dC->dU-editing enzyme APOBEC-3F antibody; Induced upon T cell activation antibody; KA6 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse
  • 免疫原：
    Recombinant Human DNA dC->dU-editing enzyme APOBEC-3F protein (139-287AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，APOBEC3F Antibody (CSB-PA816878LA01HU)，的标记方式是Non-conjugated。对于APOBEC3F Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA816878LB01HU APOBEC3F Antibody, HRP conjugated ELISA
    FITC CSB-PA816878LC01HU APOBEC3F Antibody, FITC conjugated
    Biotin CSB-PA816878LD01HU APOBEC3F Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Exhibits antiviral activity against Vif-deficient HIV-1. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity also against hepatitis B virus (HBV), equine infectious anemia virus (EIAV), xenotropic MuLV-related virus (XMRV) and simian foamy virus (SFV) and may inhibit the mobility of LTR and non-LTR retrotransposons. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.
  • 基因功能参考文献：
    1. virus adaptation and computational studies to interrogate the APOBEC3F-Vif interface and build a robust structural model; taken together with mutagenesis results, propose a wobble model to explain how HIV-1 Vif has evolved to bind different APOBEC3 enzymes PMID: 26628363
    2. Overexpression of APOBEC3F in tumor tissues is potentially predictive for poor recurrence-free survival from hepatitis b virus-hepatocellular carcinoma patients. PMID: 26760979
    3. Our results provide genetic epidemiological evidence that A3F(APOBEC3F ) modulates HIV-1/AIDS disease progression PMID: 26942578
    4. Six residues located within the conserved HIV-1 Vif F1-, F2-, and F3-box motifs are essential for both APOBEC3C and APOBEC3F degradation, and an additional four residues are uniquely required for APOBEC3F degradation. PMID: 26537685
    5. This study showed for the first time a high level of APOBEC3F/3G editing in HIV-2 sequences from antiretroviral-naive patients. PMID: 25985400
    6. The nucleocapsid domain of HIV-1 Gag and a linker sequence between the two cytidine deaminase domains are required for viral packaging of APOBEC3F. PMID: 25038404
    7. s found that one pair of leucines in each of APOBEC3F's C-terminal and N-terminal cytidine deaminase domains jointly determined the degree of localization of APOBEC3F into HIV-1 virion cores. PMID: 25505075
    8. This approach identified the alpha3 and alpha4 helices of human APOBEC3F as important determinants of the interaction with HIV-1 Vif. PMID: 25142588
    9. Catalytic activity of APOBEC3F is required for efficient restriction of Vif-deficient human immunodeficiency virus 1. PMID: 24503066
    10. Analysis of the A3F (W126A L306A) double mutant revealed that both residues are required for full anti-HIV function PMID: 22451677
    11. the antiviral activity of endogenous A3F is negligible compared to that of A3G. PMID: 20702622
    12. Long-term restriction by APOBEC3F selects human immunodeficiency virus type 1 variants with restored Vif function. PMID: 20686027
    13. Alternative splicing of A3F mRNA generates truncated antiviral proteins that differ sharply in their sensitivity to Vif. PMID: 20624919
    14. These results suggest that APOBEC3F neutralization is dispensable for HIV-1 replication in primary human T-lymphocytes. PMID: 20592068
    15. The fact that several highly conserved tryphtophan residues in Vif are specifically required for the suppression of APOBEC3F (A3F) but not that of APOBEC3G (A3G) suggests a critical role for A3F in the restriction of HIV-1 in vivo. PMID: 16501124
    16. APOBEC3B and APOBEC3F have roles in inhibiting L1 retrotransposition by a DNA deamination-independent mechanism PMID: 16648136
    17. The Chinese population had a higher frequency of small alleles and showed a difference in allelic structure and frequency distribution in apolipoprotein B from European and American in this populations. PMID: 16767679
    18. separation of function of the cytidine deaminase domains is maintained in hA3B and hA3F, but roles of the two domains are reversed in mouse A3 PMID: 17020885
    19. Studies focused mainly on APOBEC3F imply that it occurs associated with mRNA-PABP1 in translationally active polysomes and to a lesser extent in mRNA processing bodies (P-bodies). PMID: 17977970
    20. The APOBEC3F domain that interacts with the Vif DRMR region was located between amino acids 283 and 300 of A3F. PMID: 19036809

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  • 亚细胞定位：
    Cytoplasm. Cytoplasm, P-body.
  • 蛋白家族：
    Cytidine and deoxycytidylate deaminase family
  • 组织特异性：
    Widely expressed. Highly expressed in ovary.
  • 数据库链接：

    HGNC: 17356

    OMIM: 608993

    KEGG: hsa:200316

    STRING: 9606.ENSP00000309749

    UniGene: Hs.659809