Your Good Partner in Biology Research

  1. Home
  2. 抗体
  3. 多克隆抗体
  4. ARHGEF18 Antibody

ARHGEF18 Antibody

  • 货号：
    CSB-PA019681HA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Immunofluorescence staining of PC3 cells with CSB-PA019681HA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • IHC image of CSB-PA019681HA01HU diluted at 1:200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) ARHGEF18 Polyclonal antibody
  • Uniprot No.：
    Q6ZSZ5
  • 基因名：
    ARHGEF18
  • 别名：
    114 kDa Rho specific guanine nucleotide exchange factor antibody; 114 kDa Rho-specific guanine nucleotide exchange factor antibody; ARHGEF 18 antibody; Arhgef18 antibody; ARHGI_HUMAN antibody; KIAA0521 antibody; MGC15913 antibody; p114 Rho GEF antibody; p114-Rho-GEF antibody; p114RhoGEF antibody; Rho guanine nucleotide exchange factor 18 antibody; Rho specific guanine nucleotide exchange factor p114 antibody; Rho/Rac guanine nucleotide exchange factor (GEF) 18 antibody; Rho/Rac guanine nucleotide exchange factor 18 antibody; SA RhoGEF antibody; SA-RhoGEF antibody; Septin associated RhoGEF antibody; Septin-associated RhoGEF antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Rho guanine nucleotide exchange factor 18 protein (159-551AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，ARHGEF18 Antibody (CSB-PA019681HA01HU)，的标记方式是Non-conjugated。对于ARHGEF18 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA019681HB01HU ARHGEF18 Antibody, HRP conjugated ELISA
    FITC CSB-PA019681HC01HU ARHGEF18 Antibody, FITC conjugated
    Biotin CSB-PA019681HD01HU ARHGEF18 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. Its activation induces formation of actin stress fibers. Also acts as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production. Required for EPB41L4B-mediated regulation of the circumferential actomyosin belt in epithelial cells.
  • 基因功能参考文献：
    1. mutation of ARHGEF18 is a likely cause of inherited retinal dystrophy PMID: 28132693
    2. CRB3A recruits p114RhoGEF and its activator Ehm2 to the cell periphery using both functional motifs of its cytoplasmic tail and increases RhoA activation levels. PMID: 26217016
    3. LKB1 and p114RhoGEF control RhoA activity in bronchial epithelial cells to promote apical junction assembly. PMID: 23648482
    4. Stimulation of cortical myosin phosphorylation by p114RhoGEF drives cell migration and tumor cell invasion. PMID: 23185572
    5. In its regulation of the circumferential actomyosin tensile system, Lulu2 interacts with and activates p114RhoGEF at apical cell-cell junctions. PMID: 22006950
    6. Data indicate that p114RhoGEF is a component of a junction-associated Rho signalling module that drives spatially restricted activation of RhoA to regulate junction formation and epithelial morphogenesis. PMID: 21258369
    7. p114-RhoGEF and Lfc are critically involved in Wnt-3a- and Dvl-induced RhoA activation and neurite retraction in N1E-115 cells. PMID: 20810787
    8. data showed that the novel guanine nucleotide exchange factor p114RhoGEF regulates the activity of RhoA and Rac1, and that Gbetagamma subunits of heterotrimeric G proteins are activators of p114RhoGEF under physiological conditions PMID: 14512443

    显示更多

    收起更多

  • 相关疾病：
    Retinitis pigmentosa 78 (RP78)
  • 亚细胞定位：
    Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Apical cell membrane.
  • 组织特异性：
    Expressed in all tissues tested with highest expression in kidney and pancreas. Weakly or not expressed in liver, skeletal muscle and testis. Isoform 1: Expressed in eosinophils. Isoform 2: Expressed in eosinophils. Isoform 3: Expressed in eosinophils. Is
  • 数据库链接：

    HGNC: 17090

    OMIM: 616432

    KEGG: hsa:23370

    STRING: 9606.ENSP00000352995

    UniGene: Hs.465761