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ARID3A Antibody

  • 货号：
    CSB-PA858728LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Mouse liver tissue
      All lanes: ARID3A antibody at 2.7μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 63 kDa
      Observed band size: 63 kDa
    • IHC image of CSB-PA858728LA01HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA858728LA01HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA858728LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) ARID3A Polyclonal antibody
  • Uniprot No.：
    Q99856
  • 基因名：
    ARID3A
  • 别名：
    ARI3A_HUMAN antibody; ARID domain containing 3A antibody; ARID domain-containing protein 3A antibody; ARID3A antibody; AT rich interactive domain 3A (BRIGHT- like) protein antibody; AT rich interactive domain 3A (BRIGHT-like) antibody; AT rich interactive domain-containing protein 3A antibody; AT-rich interactive domain-containing protein 3A antibody; B cell regulator of IgH transcription antibody; B-cell regulator of IgH transcription antibody; Bright antibody; dead ringer like 1 antibody; dead ringer; Drosophila; homolog-like 1 antibody; Dead ringer-like protein 1 antibody; DRIL1 antibody; DRIL3 antibody; DRX antibody; E2F binding protein 1 antibody; E2F-binding protein 1 antibody; E2FBP1 antibody; Homo sapiens AT rich interactive domain 3A (BRIGHT-like) antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse
  • 免疫原：
    Recombinant Human AT-rich interactive domain-containing protein 3A protein (26-137AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，ARID3A Antibody (CSB-PA858728LA01HU)，的标记方式是Non-conjugated。对于ARID3A Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA858728LB01HU ARID3A Antibody, HRP conjugated ELISA
    FITC CSB-PA858728LC01HU ARID3A Antibody, FITC conjugated
    Biotin CSB-PA858728LD01HU ARID3A Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Transcription factor which may be involved in the control of cell cycle progression by the RB1/E2F1 pathway and in B-cell differentiation.
  • 基因功能参考文献：
    1. Data indicate ARID3a(+) B cells as a type of effector B cell, and link ARID3a expression in B lymphocytes to interferon alpha (IFNa)-associated inflammatory responses in systemic lupus erythematosus (SLE). PMID: 27522115
    2. results suggest that appropriate regulation of ARID3a is critical for normal development of both myeloid and B lineage pathways. PMID: 26685208
    3. These data reveal new functions for ARID3a in early hematopoiesis and suggest that knowledge regarding ARID3a levels in HSPCs could be informative for applications requiring transplantation of those cells. PMID: 25535283
    4. Systemic lupus erythematosus (SLE) patients had increased ARID3a+ B cells compared to healthy controls. ARID3a was not expressed in naive B cells of controls, but was abundant in SLE patients. Number of ARID3a+ B cells correlated with disease activity. PMID: 25185498
    5. miR-125b can act as an oncogene in B-cell acute lymphoblastic leukemia by targeting ARID3a and mediating its repression. PMID: 22469780
    6. These findings support the hypothesis that Epstein-Barr virus EBNA1 initiates transcription at the C promoter via interactions between multiple EBNA1 homodimers and cellular transcription such as E2F1, ARID3A and Oct-2. PMID: 22302879
    7. These results indicate both cooperative and interdependent roles for ARID3A and p53 in the transcriptional activation of p21(WAF1) in response to DNA damage. PMID: 22172947
    8. functions as a critical antagonist to the p16(INK4A)-Rb tumor suppressor machinery by regulating promyelocytic leukemia protein stability PMID: 22010578
    9. report that E2FBP1 inhibits accumulation of ICP0 RNA and, at the same time, is degraded via ICP0's herpes ubiquitin ligase 2 (HUL-2) activity upon HSV-1 infection. PMID: 21248039
    10. Bright/ARID3a inhibition causes increased developmental plasticity in mouse and human cells. PMID: 20680960
    11. Solution NMR structure of the ARID domain of human ARID3A. PMID: 20455271
    12. Results show that DRIL1 disrupts cellular protection against RAS(V12)-induced proliferation downstream of the p19(ARF)/p53 pathway. PMID: 11812999
    13. role in p53 regulatory pathway.(E2FBP1) PMID: 12136662
    14. Variations in Bright binding and matrix attachment region activity contribute to localized control of accessibility and therefore nonrandom gene use during V(D)J recombination. PMID: 12193717
    15. a putative p53-binding site was found, which specifically responded to p53, in the second intron of the E2FBP1/DRIL1 gene PMID: 12692263
    16. E2FBP1 modulates cell growth through down-regulation of promyelocytic leukemia bodies. PMID: 15017387
    17. Bright is not expressed in all human B lymphocyte subpopulations. PMID: 15203319
    18. TFII-I directly interacts with Bright through amino acids in Bright's protein interaction domain PMID: 16738337
    19. identify Bright as a contributor to accessibility of the IgH enhancer PMID: 17386101
    20. Id1 inhibited DNA binding by Dril1, and the two proteins co-localized in vitro and in vivo, providing a potential mechanism for suppression of fibrosis by Id1 through inhibition of the profibrotic function of Dril1. PMID: 18583319
    21. A palmitoylated pool of Bright is diverted to lipid rafts of resting B cells where it associates with signalosome components. PMID: 19214191

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Note=Shuttles between nucleus and cytoplasm.
  • 组织特异性：
    Widely expressed, with highest expression in skeletal muscle, thalamus, and colon.
  • 数据库链接：

    HGNC: 3031

    OMIM: 603265

    KEGG: hsa:1820

    STRING: 9606.ENSP00000263620

    UniGene: Hs.501296