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ATG3 Antibody

  • 货号：
    CSB-PA777675
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA777675(ATG3 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
    • The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using CSB-PA777675(ATG3 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
    • Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-3: K562 cells, mouse kidney tissue, Raji cells, Primary antibody: CSB-PA777675(ATG3 Antibody) at dilution 1/325, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds
  • 其他：

产品详情

  • Uniprot No.：
    Q9NT62
  • 基因名：
  • 别名：
    2610016C12Rik antibody; Apg 3 antibody; APG3 antibody; APG3 autophagy 3 like antibody; APG3 like antibody; APG3, S. cerevisiae, homolog of antibody; APG3-like antibody; APG3L antibody; Apg3p antibody; ATG 3 antibody; ATG3 antibody; ATG3 autophagy related 3 homolog antibody; ATG3 autophagy related 3 homolog (S. cerevisiae) antibody; ATG3_HUMAN antibody; Autophagy 3, S. cerevisiae, homolog of antibody; Autophagy Apg3p/Aut1p like antibody; autophagy related 3 antibody; Autophagy related protein 3 antibody; Autophagy-related protein 3 antibody; DKFZp564M1178 antibody; FLJ22125 antibody; hApg3 antibody; MGC15201 antibody; OTTHUMP00000214547 antibody; OTTHUMP00000214548 antibody; PC3 96 antibody; Protein PC3-96 antibody; Ubiquitin-like-conjugating enzyme ATG3 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Fusion protein of Human ATG3
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:2000-1:5000
    WB 1:500-1:2000
    IHC 1:25-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.
  • 基因功能参考文献：
    1. ATG3 was targeted by miR-23a, suggesting that ATG3 is an autophagy-related competing endogenous RNA. PMID: 30007957
    2. Results revealed that miR-16 was significantly downregulated, and ATG3 was significantly upregulated in non-small cell lung carcinoma (NSCLC) patient tissue samples. ATG3 was found to be a direct target of miR-16. PMID: 29138833
    3. miR-155 silencing rescued autophagosome number in Mycobacterium tuberculosis infected dendritic cells and enhanced autolysosome fusion, thereby supporting a previously unidentified role of the miR-155 as inhibitor of ATG3 expression. PMID: 29300789
    4. PTK2 inhibition-induced sustained levels of ATG3 were able to sensitize cancer cells to DNA-damaging agents. PMID: 28103122
    5. Our data demonstrate that HOTAIR promotes the activation of autophagy in HCC cells by upregulating the expression of the autophagy-related genes ATG3 and ATG7. PMID: 27301338
    6. ATG3 upregulation contributes to autophagy induced by the detachment of intestinal epithelial cells from the extracellular matrix, but promotes autophagy-independent apoptosis of the attached cells PMID: 26061804
    7. Hidden Markov models were used to detect protein homology among the flexible regions of Atg3 homologs and importance of conserved regions evaluated by performing affinity capture experiments with human Atg3 deletion constructs; binding studies and competition experiments demonstrate that overlapping sites in the Atg3FR are important for E3 binding and E1 binding. PMID: 24186333
    8. The region of human ATG3 that interacts with ATG7 is precisely identified using nuclear magnetic resonance. PMID: 26043688
    9. ATG3 gene and its gene family may play an important role in transformation of myelodysplastic syndrome. PMID: 24420857
    10. Lipidation of the LC3/GABARAP family of autophagy proteins relies on a membrane-curvature-sensing domain in Atg3. PMID: 24747438
    11. 13 residues of the ATG3 fragment form a short beta-strand followed by an alpha-helix on a surface area that is an exclusive binding site for ATG12. PMID: 24191030
    12. caspase-8 overexpression led to Atg3 degradation and this event depended on caspase-8 enzymatic activity PMID: 22644571
    13. These results unveil a role for ATG12-ATG3 in mitochondrial homeostasis and implicate the ATG12 conjugation system in cellular functions distinct from the early steps of autophagosome formation. PMID: 20723759
    14. Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p PMID: 11825910
    15. Murine Atg8L/Apg8L modification is mediated by human Atg3. PMID: 16704426

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    ATG3 family
  • 组织特异性：
    Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
  • 数据库链接：

    HGNC: 20962

    OMIM: 609606

    KEGG: hsa:64422

    STRING: 9606.ENSP00000283290

    UniGene: Hs.477126