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ATG4B Antibody

  • 货号：
    CSB-PA138409
  • 规格：
    ￥2024
  • 图片：
    • Western blot analysis of extracts from Jurkat cells, using ATG4B antibody.
    • Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using ATG4B antibody.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) ATG4B Polyclonal antibody
  • Uniprot No.：
    Q9Y4P1
  • 基因名：
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthesized peptide derived from internal of Human ATG4B.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:3000
    IHC 1:50-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
  • 基因功能参考文献：
    1. ATG4B expression was observed markedly upregulated by EWS-FLI1 overexpression, and silencing of ATG4B dramatically inhibits autophagy in Ewing sarcoma cells. PMID: 28902354
    2. study demonstrates that Rab7b regulates LC3 processing by modulating Atg4B activity; taken together, findings reveal Rab7b as a novel negative regulator of autophagy through its interaction with Atg4B PMID: 28835545
    3. ATG4B contains a C-terminal LIR motif important for binding and efficient cleavage of mammalian orthologs of yeast Atg8 PMID: 28287329
    4. O-GlcNAcylation of ATG4B regulates autophagy activation by increasing its proteolytic activity under metabolic stress condition PMID: 27527864
    5. these results demonstrate a novel association of ATG4B positive expression with HER2 positive breast cancers PMID: 27556700
    6. Knockdown of ATG4B had only a minor effect on AMPK activation and G1 phase arrest in liver kinase B1-deficient or AMPK-inhibited cancer cells. PMID: 29169176
    7. Our work describes an MST4-ATG4B signaling axis that influences glioblastoma autophagy and malignancy PMID: 29232556
    8. Data show that ULK1, a protein kinase activated at the autophagosome formation site, phosphorylates human ATG4B on serine 316. PMID: 28821708
    9. Reporter assay showed that HSF1 increased the transcriptional activity of ATG4B gene promoter, and chromatin immunoprecipitation assay verified that HSF1 bound to the site (-1429 to -1417) in ATG4B gene promoter region. PMID: 28889000
    10. The structure and regulatory machinery of Atg4b [review] PMID: 28253956
    11. MIRlet-7i is able to regulate autophagic activity via regulating Atg4B expression, which might contribute to the pathogenesis of pre-eclampsia. PMID: 27770612
    12. Results demonstrated that upregulation of miR-34a by transfection or demethylation resulted in the enhanced apoptosis and drug sensitivity in prostate cancer cells. ATG4B, directly regulated by miR-34a through AMPK/mTOR, was involved in this process. PMID: 26499184
    13. Purified ATG4B protein interact with LC3B in vitro. PMID: 25652856
    14. ATG4B independently plays a role as a positive regulator on tumor proliferation and a negative regulator on autophagy in colorectal cancer cells. PMID: 24991826
    15. This study identifies ATG4B as a potential biomarker for predicting therapeutic response in treatment-naive chronic myeloid leukemia stem/progenitor cells and uncovers ATG4B as a possible drug target in these cells. PMID: 24755409
    16. The actions of ATG4 family members (particularly ATG4B) are required for the control of autophagosome fusion with late, degradative compartments in differentiating human erythroblasts. PMID: 23508006
    17. HsAtg4B negatively regulates the localization of LC3 to a membrane compartment by delipidation PMID: 15187094
    18. analysis of specificity and catalysis of HsAtg4B PMID: 16183633
    19. crystal structure of human Atg4b by X-ray crystallography at 2.0 A resolution, and show that Atg4b is a cysteine protease whose active catalytic triad site consists of Cys74, His280 and Asp278 PMID: 16325851
    20. Murine Atg8L/Apg8L modification is mediated by human Atg4B. PMID: 16704426
    21. The crystal structures of catalytically inert human Atg4B in complex with processed and unprocessed forms of LC3 showed that, on LC3 binding, the regulatory loop and the N-terminal tail of HsAtg4B undergo large conformational changes. PMID: 19322194

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Peptidase C54 family
  • 数据库链接：

    HGNC: 20790

    OMIM: 611338

    KEGG: hsa:23192

    STRING: 9606.ENSP00000384259

    UniGene: Hs.283610