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CARD8 Antibody

  • 货号：
    CSB-PA148420
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA148420(CARD8 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
    • The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA148420(CARD8 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
  • 其他：

产品详情

  • Uniprot No.：
    Q9Y2G2
  • 基因名：
  • 别名：
    Apoptotic protein NDPP 1 antibody; Apoptotic protein NDPP1 antibody; CARD 8 antibody; CARD inhibitor of NF kappa B activating ligand antibody; CARD inhibitor of NF kappaB activating ligand antibody; CARD inhibitor of NF kappaB activating ligands antibody; CARD-inhibitor of NF-kappa-B-activating ligand antibody; CARD8 antibody; CARD8_HUMAN antibody; CARDINAL antibody; Caspase recruitment domain containing protein 8 antibody; Caspase recruitment domain family member 8 antibody; Caspase recruitment domain protein 8 antibody; Caspase recruitment domain-containing protein 8 antibody; DACAR antibody; Dakar antibody; DKFZp779L0366 antibody; FLJ18119 antibody; FLJ18121 antibody; KIAA0955 antibody; MGC57162 antibody; NDPP 1 antibody; NDPP antibody; NDPP1 antibody; TUCAN antibody; Tumor up regulated CARD containing antagonist of CASP9 antibody; Tumor up regulated CARD containing antagonist of caspase 9 antibody; Tumor up-regulated CARD-containing antagonist of CASP9 antibody; tumor up-regulated CARD-containing antagonist of caspase nine antibody; Tumor upregulated CARD containing antagonist of CASP9 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Fusion protein of Human CARD8
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:1000-1:2000
    IHC 1:25-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Inflammasome sensor, which mediates inflammasome activation in response to various pathogen-associated signals, leading to subsequent pyroptosis of CD4(+) T-cells and macrophages. Inflammasomes are supramolecular complexes that assemble in the cytosol in response to pathogens and other damage-associated signals and play critical roles in innate immunity and inflammation. Acts as a recognition receptor (PRR): recognizes specific pathogens and other damage-associated signals, such as HIV-1 protease activity or Val-boroPro inhibitor, and mediates CARD8 inflammasome activation. In response to pathogen-associated signals, the N-terminal part of CARD8 is degraded by the proteasome, releasing the cleaved C-terminal part of the protein (Caspase recruitment domain-containing protein 8, C-terminus), which polymerizes to initiate the formation of the inflammasome complex: the CARD8 inflammasome directly recruits pro-caspase-1 (proCASP1) independently of PYCARD/ASC and promotes caspase-1 (CASP1) activation, which subsequently cleaves and activates inflammatory cytokines IL1B and IL18 and gasdermin-D (GSDMD), leading to pyroptosis. Ability to sense HIV-1 protease activity leads to the clearance of latent HIV-1 in patient CD4(+) T-cells after viral reactivation; in contrast, HIV-1 can evade CARD8-sensing when its protease remains inactive in infected cells prior to viral budding. Also acts as a negative regulator of the NLRP3 inflammasome. May also act as an inhibitor of NF-kappa-B activation.; Constitutes the precusor of the CARD8 inflammasome, which mediates autoproteolytic processing within the FIIND domain to generate the N-terminal and C-terminal parts, which are associated non-covalently in absence of pathogens and other damage-associated signals.; Regulatory part that prevents formation of the CARD8 inflammasome: in absence of pathogens and other damage-associated signals, interacts with the C-terminal part of CARD8 (Caspase recruitment domain-containing protein 8, C-terminus), preventing activation of the CARD8 inflammasome. In response to pathogen-associated signals, this part is ubiquitinated by the N-end rule pathway and degraded by the proteasome, releasing the cleaved C-terminal part of the protein, which polymerizes and forms the CARD8 inflammasome (Probable).; Constitutes the active part of the CARD8 inflammasome. In absence of pathogens and other damage-associated signals, interacts with the N-terminal part of CARD8 (Caspase recruitment domain-containing protein 8, N-terminus), preventing activation of the CARD8 inflammasome. In response to pathogen-associated signals, the N-terminal part of CARD8 is degraded by the proteasome, releasing this form, which polymerizes to form the CARD8 inflammasome complex: the CARD8 inflammasome complex then directly recruits pro-caspase-1 (proCASP1) and promotes caspase-1 (CASP1) activation, leading to gasdermin-D (GSDMD) cleavage and subsequent pyroptosis.
  • 基因功能参考文献：
    1. this study showed that the CARD8-C10X (rs2043211) AT genotype contributed to the susceptibility of multiple myeloma PMID: 30211233
    2. Our results showed that rs10754558 NLRP3 and rs2043211 CARD8 polymorphisms are associated with rheumatoid arthritis development (p value = 0.044, OR = 1.77, statistical power = 0.999) and severity measured by Health Assessment Questionnaire (HAQ) (p value = 0.03), respectively. PMID: 29230505
    3. this study found that the AT genotype of CARD8 (rs2043211) was significantly higher compared to TT genotype in high and intermediate risk chronic myeloid leukemia patients PMID: 29097263
    4. Review/Meta-analysis: CARD8 p.C10X SNP were not associated with the susceptibility to rheumatoid arthritis. PMID: 28185410
    5. these results identify a new CARD8 variant associated with periodic fever with aphthous stomatitis, pharyngitis, and cervical adenitis, and further suggest that disruption of the interaction between CARD8 and NLRP3 can regulate autoinflammation in patients PMID: 28137891
    6. The over expression of CARD8 due to higher promoter activity of the TT genotype may result in a dramatically inflammatory immune response and then increase the susceptibility to Arteriosclerosis Obliterans. PMID: 28135700
    7. High CARD8 expression is associated with malignant melanoma. PMID: 27810076
    8. The CARD8 rs2043211 genetic variants was not implicated in the development of gout in the male Korean population. However, we found that in a pair-wise comparison of the CA/TT P2X7R and CARD8 genotype combination was shown to have an increased trend for the risk of gout. PMID: 27550484
    9. The NLRP3 rs10754558 gene polymorphism was significantly associated with the occurrence of CAD, while the CARD8 rs2043211 gene polymorphism was not involved. PMID: 27110561
    10. In patients with ileal, stenotic or fistulizing Crohn's disease, the mutant-type CARD8 rs2043211 polymorphism may generate a potentially protective effect. (Meta-analysis) PMID: 26462578
    11. There is evidence for association of gout with functional variants in CARD8, IL1B and CD14. PMID: 26462562
    12. The polymorphism of rs2043211 in CARD8 may be a relevant host susceptibility factor for the development of preeclampsia in the Chinese Han population. PMID: 25895569
    13. A novel association between CARD8 and increased risk of surgical recurrence in Crohn's disease was observed and CARD8 could be a new marker for risk stratification and prevention of recurrent surgery. PMID: 26283210
    14. genetic polymorphism is associated with susceptibility to Crohn's disease under the dominant model and homozygote contrast in the European population; meta-analysis PMID: 25564880
    15. Patients carrying genotype TT of CARD8 rs2043211 polymorphism had higher triglycerides levels compared to those carrying the AA genotype. PMID: 25790751
    16. CARD8 might not play a role in the pathogenesis of Tourette syndrome in Chinese Han population PMID: 25921775
    17. Data indicate 3 variants in 3 novel genes myc target 1 protein (MYCT1), caspase recruitment domain family member 8 (CARD8) and zinc finger protein 543 (ZNF543), associated with familial IgA nephropathy (IgAN). PMID: 26095808
    18. we show that CARD8 plays a role as a negative regulator of NLRP3 inflammasome through its binding with NLRP3 PMID: 24517500
    19. levels of inflammasome-produced cytokines as a measure of inflammasome activation in healthy individuals carrying Q705K polymorphism in the NLRP3 gene combined with C10X in the CARD8 gene PMID: 24098386
    20. ANRIL may increase the risk of ischemic stroke through regulation of the CARD8 pathway PMID: 24385277
    21. In a Swedish population, the minor allele of CARD8-C10X is associated with a decreased risk of AS, but not with levels of faecal calprotectin or disease phenotype. PMID: 23547871
    22. The first crystal structure of the CARD8 caspase-recruitment domain is reported. It adopts a six-helix bundle fold with a unique conformation of the alpha6 helix that is described for the first time. PMID: 23695559
    23. Data indicate that CARD8 (caspase recruitment domain 8) mRNA was highly expressed in atherosclerotic plaques, and the minor allele was associated with lower expression of CARD8 in the plaques, suggesting that CARD8 may promote inflammation. PMID: 23611467
    24. The results of this study support the novel association between CARD8 gene and HIV+tuberculosis coinfection, demonstrating that inflammasome genetics could influence HIV-1 infection and the development of opportunistic infection. PMID: 23507658
    25. Mutation in the CARD8, a component of inflammasome, is associated with lower levels of antibodies directed to mannans and glucans at least in Crohn's disease patients. PMID: 23506543
    26. Results suggest that the CARD8 rs2043211 gene variant does not in susceptibility to rheumatoid arthritis (RA) or in the development of cardiovascular disease in patients with RA. PMID: 23088220
    27. Variations in the CARD8 and NLRP3 genes are not associated with rheumatoid arthritis in the French as well general Tunisian population. PMID: 22128899
    28. analysis of interaction of the inflammasome genes CARD8 and NLRP3 in abdominal aortic aneurysms PMID: 21621776
    29. Twelve single nucleotide polymorphisms within NLRP1, NLRP3, NLRC4, CARD8, CASP1, and IL1B genes were analyzed in 150 HIV-1-infected Brazilian subjects. PMID: 22227487
    30. Caspase8 polymorphism IVS12-19 G>A but not CASP8 -652 6N del polymorphism may modulate risk of esophageal squamous cell carcinoma and its survival outcome in northern Indian population. PMID: 21308686
    31. CARD8 variants might have roles in the pathogenesis of Crohn's disease and ulcerative colitis in Koreans PMID: 21248762
    32. propose that CARD8-NALP3 genotype combinations protect against gut inflammation by preventing the NALP3 inflammasome from producing excessive interleukin-1be PMID: 20182451
    33. Data show that CARD8 represents a novel molecular switch involved in the endogenous regulation of NOD2-dependent inflammatory processes in epithelial cells. PMID: 20385562
    34. Carriage of CARD8-X is associated with a worse disease course in early rheumatoid arthritis. PMID: 19443463
    35. Study shows an essential role for apoptosis signal-regulating kinase 1 (ASK1), together with both c-jun-N-terminal kinase (JNK) and p38 pathways, and caspase-8 in Fas-induced apoptosis. PMID: 19940360
    36. CARD-8 protein, a new CARD family member that regulates caspase-1 activation and apoptosis. (card-8 protein) PMID: 11821383
    37. Expression and characterization of NDPP1 PMID: 11956601
    38. TUCAN/CARDINAL and DRAL participate in a common pathway for modulation of NF-kappaB activation. PMID: 12067710
    39. TUCAN does not play a role in inhibition of procaspase-9 and in determining the sensitivity to cisplatin in Non Small Cells Lung Cancer. PMID: 16796750
    40. association between a likely functional polymorphism in TUCAN and Crohn disease PMID: 17030188
    41. no significant association between the risk allele "A" at Cys10Stop and risk for Crohn's disease or ulcerative colitis was detected in patients of German and Norwegian descent PMID: 17484912
    42. deleterious polymorphism of CARD8 may help predict the severity of rheumatoid arthritis PMID: 17878386
    43. Analyzing 3 independent European IBD cohorts, we found no evidence that the C10X variant in CARD8 confers susceptibility for CD. PMID: 18092344
    44. isoforms of CARD8 differ in their N-termini, resulting in diverse predicted molecular weights (47, 48, 51, 54 and 60 kDa) and multiple outcomes for the variant including Cys10Stop, Cys34Stop, Phe52Ile and Phe102Ile PMID: 18212821
    45. The NALP3 and TUCAN single-nucleotide polymorphisms may explain the increased IL-1beta levels and inflammatory symptoms observed, but further studies are needed to reveal a functional relationship. PMID: 18311798
    46. Women, but not men, carrying the CARD8 AA genotype (truncated protein) had a 2.39-fold higher risk of developing Alzheimer's disease than subjects with the CARD8 TT genotype (full-length protein) PMID: 18841008
    47. Gene-gene interaction between CARD8 and interleukin-6 reduces Alzheimer's disease risk. PMID: 19252766
    48. CARD8 and NALP3 genes combined polymorphism has a role of developing Crohn disease in men. PMID: 19319132

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  • 亚细胞定位：
    Cytoplasm. Nucleus.; [Caspase recruitment domain-containing protein 8, C-terminus]: Inflammasome.
  • 组织特异性：
    High expression in lung, ovary, testis and placenta. Lower expression in heart, kidney and liver. Also expressed in spleen, lymph node and bone marrow.
  • 数据库链接：

    HGNC: 17057

    OMIM: 609051

    KEGG: hsa:22900

    STRING: 9606.ENSP00000375767

    UniGene: Hs.446146