Your Good Partner in Biology Research

  1. Home
  2. 抗体
  3. 多克隆抗体
  4. CD3EAP Antibody

CD3EAP Antibody

  • 货号：
    CSB-PA004932LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, U87 whole cell lysate, Rat brain tissue
      All lanes: CD3EAP antibody at 3.9μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 55, 56 kDa
      Observed band size: 55, 56 kDa
    • IHC image of CSB-PA004932LA01HU diluted at 1:200 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA004932LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) CD3EAP Polyclonal antibody
  • Uniprot No.：
    O15446
  • 基因名：
    CD3EAP
  • 别名：
    A34.5 antibody; Anti sense to ERCC 1 protein antibody; Antisense to ERCC-1 protein antibody; ASE 1 antibody; ASE-1 antibody; ASE1 antibody; CAST antibody; CD3 epsilon associated protein antibody; CD3-epsilon-associated protein antibody; CD3E antigen, epsilon polypeptide associated protein antibody; CD3E associated protein antibody; CD3e molecule, epsilon associated protein antibody; CD3E-associated protein antibody; CD3EAP antibody; DNA directed RNA polymerase I subunit RPA34 antibody; DNA-directed RNA polymerase I subunit RPA34 antibody; MGC118851 antibody; PAF 49 antibody; PAF49 antibody; RNA polymerase I associated factor PAF49 antibody; RNA polymerase I-associated factor PAF49 antibody; RPA34_HUMAN antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Rat
  • 免疫原：
    Recombinant Human DNA-directed RNA polymerase I subunit RPA34 protein (289-404AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，CD3EAP Antibody (CSB-PA004932LA01HU)，的标记方式是Non-conjugated。对于CD3EAP Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA004932LB01HU CD3EAP Antibody, HRP conjugated ELISA
    FITC CSB-PA004932LC01HU CD3EAP Antibody, FITC conjugated
    Biotin CSB-PA004932LD01HU CD3EAP Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.; Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.
  • 基因功能参考文献：
    1. Haplotypes consisting of PPP1R13L, CD3EAP and GLTSCR1 SNPs on Chr19q13.3 may associate with lung cancer risk in the Chinese population. PMID: 30128886
    2. a genetic variant in the CD3EAP gene is associated with outcome of patients treated for multiple myeloma PMID: 26568154
    3. These findings demonstrate that 19p13.3-GADD45B rs7354 variant and interaction between 19p13.3-GADD45B rs3783501 and 19q13.3-CD3EAP rs967591 may play a role in association with smoke-exposed lung cancer among Chinese. PMID: 28870783
    4. There was no interaction between NFKB1 polymorphisms and PPP1R13L and CD3EAP and smoking status in relation to lung cancer risk PMID: 25917613
    5. In nonsmoking Chinese women CD3EAP rs967591 variant allele carriers are at increased risk of lung cancer. PMID: 23624123
    6. Polymorphism in RAI and CD3EAP are associated with outcome of myeloma patients treated with high dose treatment. PMID: 21046104
    7. model for CAST/hPAF49 function in which the network of interactions of Pol I-specific subunits with UBF facilitates conformational changes of the polymerase, leading to stabilization of the Pol I-template complex and, thereby, activation of transcription PMID: 16809778
    8. The ASE-1 polymorphism was associated with an increased risk of adenomas, OR of 1.39 (95% CI 1.06-1.81), which upon stratification was apparent among women only, OR of 1.66 (95% CI 1.15-2.39) PMID: 16817948
    9. ASE-1 polymorphisms and the previously identified ASE-1 haplotype are not associated with risk of colorectal cancer. PMID: 18289367
    10. A SNP in the 5' noncoding region of exon 1 at position -21 from the first codon, which does not affect the final AA sequence but may affect transcription/translation, was associated with drug sensitivity in the NC160 human cancer cell line panel. PMID: 19396015
    11. ASE-1 co-localises with the RNA polymerase I transcription initiation factor UBF/NOR-90 throughout all stages of the cell cycle and these two proteins associate with each other in vitro. PMID: 9426281

    显示更多

    收起更多

  • 亚细胞定位：
    Nucleus, nucleolus. Chromosome. Note=Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes.
  • 蛋白家族：
    Eukaryotic RPA34 RNA polymerase subunit family
  • 数据库链接：

    HGNC: 24219

    OMIM: 107325

    KEGG: hsa:10849

    STRING: 9606.ENSP00000310966

    UniGene: Hs.710495