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  4. CDK8 Antibody

CDK8 Antibody

  • 中文名称：
    CDK8兔多克隆抗体
  • 货号：
    CSB-PA162953
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA162953(CDK8 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
  • 其他：

产品详情

  • Uniprot No.：
    P49336
  • 基因名：
    CDK8
  • 别名：
    cdk8 antibody; CDK8 protein kinase antibody; CDK8_HUMAN antibody; Cell Division Kinase 8 antibody; Cell division protein kinase 8 antibody; Cyclin Dependent kinase 8 antibody; Cyclin-dependent kinase 8 antibody; K35 antibody; Mediator complex subunit cdk8 antibody; Mediator of RNA polymerase II transcription subunit cdk8 antibody; Protein kinase K35 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthetic peptide of Human CDK8
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:2000-1:5000
    IHC 1:25-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.
  • 基因功能参考文献：
    1. these observations suggest that Zyxin promotes colon cancer tumorigenesis in a mitotic-phosphorylation-dependent manner and through CDK8-mediated YAP activation. PMID: 29967145
    2. Genes coregulated by CDK8/19 and NFkappaB include IL8, CXCL1, and CXCL2. PMID: 28855340
    3. Moreover, it identified CDK19 and CDK8 to be specifically overexpressed during prostate cancer progression, highlighting their potential as novel therapeutic targets in advanced prostate cancer. PMID: 27678455
    4. identification of the Mediator-associated kinase CDK8, and its paralog CDK19, as negative regulators of IL-10 production during innate immune activation PMID: 28805801
    5. These results identify CDK8 as a novel downstream mediator of estrogen receptors (ER) and suggest the utility of CDK8 inhibitors for ER-positive breast cancer therapy PMID: 28147342
    6. Our data suggest for the first time that CDK8 appears to contribute to the malignant mechanism of LSCC and may represent a significant prognostic marker for LSCC patients. PMID: 28220308
    7. On stimulation of TLR9, CDK8/19 positively regulates inflammatory gene transcription in cooperation with NF-kappaB and C/EBPbeta. PMID: 28151579
    8. Data suggest that, during neurogenesis, Mediator complex cyclin-dependent kinases (CDK8, CDK19) interact directly with PRC2 (polycomb repressive complex 2) subunit EZH2 (enhancer of zeste homolog 2), as well as SUZ12 (suppressor of zeste 12 homolog). PMID: 26002960
    9. the expression of CDK8 and its interactive genes has a profound impact on the response to adjuvant therapy in breast cancer in accordance with the role of CDK8 in chemotherapy-induced tumor-supporting paracrine activities--{REVIEW} PMID: 26452386
    10. Data suggest that MED13, MED12, CDK8 and cyclin C (CycC) comprise a four-subunit "kinase" module of the Mediator complex that functions as a major ingress of oncogenic and developmental signaling/gene expression in humans. [REVIEW] PMID: 26182352
    11. our data provided evidence that CDK-8 played a significant role in colon cancer hepatic metastasis by regulating the Wnt/beta-catenin signal pathway PMID: 26349978
    12. Collectively, our results implicate Ste12 and Tec1 as general and important contributors to the Cdk8, RNAPII-CTD regulatory circuitry as it relates to the maintenance of genome integrity PMID: 26496706
    13. miR-101 is a potent tumor repressor that directly represses CDK8 expression PMID: 26286725
    14. Mediator-associated kinases CDK8 and CDK19 restrain increased activation of key super-enhancer-associated genes in acute myeloid leukaemia (AML) cells PMID: 26416749
    15. The Skp2-mH2A1-CDK8 axis has a critical role in breast cancer development via dysregulation of the G2/M transition, polyploidy, cell growth dysregulation, and loss of tumor suppression. PMID: 25818643
    16. Current state of the art confirms that further development of CDK8 inhibitors will translate into targeted therapies in oncology PMID: 26006748
    17. Microarrays identified target genes for each CDK, and we selected six genes: two target genes of CDK8, two target genes of CDK19 and two genes that were targets for both. PMID: 22117896
    18. Suggest that miR-107 plays a key role in cisplatin resistance by targeting the CDK8 protein in non small cell lung cancer cells. PMID: 25400821
    19. A novel transcriptional repression mechanism of hMED18 mediated by hCDK8 and further a novel positive role of free CDK/cyclin module in transcriptional activation is described. PMID: 24840924
    20. CDK8 regulates the transcription factors turnover, C-terminal domain phosphorylation, and regulates the activator or repressor functions. (Review) PMID: 24139904
    21. Cdk8 is required for stress-induced Mdv1-Dnm1 interaction and mitochondrial fragmentation. PMID: 24439911
    22. Here, loss of CDK8 suppressed the reduced mRNA expression and RNAPII occupancy levels of CTD truncation mutants. PMID: 24009531
    23. CDK8 and CDK19, individually interact with PRMT5 and WDR77, and their interactions with PRMT5 cause transcriptional repression of C/EBPbeta target genes. PMID: 23749998
    24. The phosphorylation of S375 by CDK8 regulates E2F1 ability to repress transcription of beta-catenin/TCF-dependent genes, as well as activation of E2F1-dependent genes. PMID: 22945643
    25. results show a reverse correlation between CDK8 levels and several key features of the endometrial cancer cells, including cell proliferation, migration and invasion as well as tumor formation in vivo PMID: 23454913
    26. Study reports that HIF1A employs a specific variant of the Mediator complex to stimulate RNAPII elongation; the Mediator-associated kinase CDK8, but not the paralog CDK19, is required for induction of many HIF1A target genes. PMID: 23746844
    27. s found that interaction of the CDK8 kinase module with Mediator's middle module interferes with C-terminal domain-dependent RNAPII binding to a previously unknown middle-module CTD-binding site and with the holoenzyme formation process. PMID: 23563140
    28. CDK8 is a key regulator of STAT1 and antiviral responses. PMID: 23352233
    29. 2.2-A crystal structure of CDK8/CycC in complex with sorafenib; the CDK8 activation loop appears not to be phosphorylated. Based on the structure, we discuss an alternate mode of CDK8 activation to the general CDK activation by T-loop phosphorylation PMID: 21806996
    30. Cyclin-dependent kinase 8 mediates chemotherapy-induced tumor-promoting paracrine activities PMID: 22869755
    31. Retroviral cyclin enhances cyclin-dependent kinase-8 activity. PMID: 22379099
    32. we identify a role for the CDK8 oncogene in regulating tumor differentiation and stem cell pluripotency PMID: 22345154
    33. beta-catenin expression was suppressed by CDK8 interference in the gastric adenocarcinoma cell lines. PMID: 21344156
    34. significant inverse correlation between mH2A and CDK8 expression levels exists in melanoma patient samples PMID: 21179167
    35. CDK8 may identify a subset of colon cancer patients with poor prognosis. PMID: 20514474
    36. plays a role in mechanisms of transcriptional regulation upon protein phosphorylation. (review) PMID: 20408451
    37. Overexpression of CDK8 is associated with beta-catenin activation and colon cancer. PMID: 19790197
    38. The s show, using a human tumor cell line, that CDK8 is a positive regulator of genes within the serum response network, including several members of the activator protein 1 and early growth response family of oncogenic transcription factors. PMID: 20098423
    39. CDK8 play positive roles in transcriptional activation. PMID: 17212659
    40. RNA interference experiments demonstrate that CDK8 functions as a coactivator within the p53 transcriptional program. PMID: 17612495
    41. Within T/G-Mediator, cdk8 phosphorylates serine-10 on histone H3, which in turn stimulates H3K14 acetylation by GCN5L within the complex. PMID: 18418385
    42. by retaining RB1 and amplifying CDK8, colorectal tumour cells select conditions that collectively suppress E2F1 and enhance the activity of beta-catenin PMID: 18794899
    43. CDK8 kinase activity was necessary for beta-catenin-driven transformation and for expression of several beta-catenin transcriptional targets PMID: 18794900
    44. Med12--but not Med13--is essential for activating the CDK8 kinase. PMID: 19047373
    45. Med12 and Med13 are critical for human CDK8 subcomplex -dependent repression, whereas CDK8 kinase activity is not PMID: 19240132

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily
  • 数据库链接：

    HGNC: 1779

    OMIM: 603184

    KEGG: hsa:1024

    STRING: 9606.ENSP00000370938

    UniGene: Hs.382306