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CENPF Antibody

  • 货号：
    CSB-PA646140
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA646140(CENPF Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
    • The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA646140(CENPF Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
  • 其他：

产品详情

  • Uniprot No.：
    P49454
  • 基因名：
  • 别名：
    AH antigen antibody; Cell cycle dependent 350K nuclear protein antibody; CENF antibody; CENP F antibody; CENP F kinetochore protein antibody; CENP-F antibody; CENPF antibody; CENPF kinetochore protein antibody; CENPF_HUMAN antibody; Centromere protein F 350/400ka antibody; Centromere protein F antibody; Centromere protein F, 350/400kDa antibody; CILD31 antibody; Hcp 1 antibody; Hcp1 antibody; Kinetochore protein CENP F antibody; Kinetochore protein CENPF antibody; Mitosin antibody; PRO1779 antibody; STROMS antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Synthetic peptide of Human CENPF
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:1000-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia.
  • 基因功能参考文献：
    1. This is the second case report identifying CENPF mutation as the cause of Stromme syndrome PMID: 28407396
    2. s suggest that CENP-F might act as a transporter of mitochondria and other cellular cargoes by attaching them to dynamic microtubule ends during both polymerization and depolymerization of tubulin. PMID: 28701340
    3. Tumors with higher topoisomerase IIalpha and/or mitosin expression have a higher risk of recurrence after initial treatment, and these patients may benefit from adjuvant treatment and closer radiological follow-up PMID: 28301542
    4. We show for the first time that Stromme syndrome is an autosomal-recessive disease caused by mutations in CENPF that can result in a wide phenotypic spectrum. PMID: 26820108
    5. Miro and Cenp-F promote anterograde mitochondrial movement and proper mitochondrial distribution in daughter cells. PMID: 26259702
    6. Mitosin and pHH3 immunostaining predict poorer survival in astrocytomas WHO grades II and III. PMID: 26188054
    7. Our data identify CENPF as a new centriolar disease gene implicated in severe human ciliopathy and microcephaly related phenotypes PMID: 25564561
    8. the increased expression of CENPF plays an important role in the progression of PCa. PMID: 25647485
    9. N-terminal microtubule-binding domain of CENP-F prefers curled oligomers of tubulin relative to microtubule walls by approximately fivefold, suggesting it may contribute to the firm bonds between kinetochores and flared plus ends of dynamic microtubules PMID: 26101217
    10. FOXM1 and CENPF function synergistically to promote tumor growth by coordinated regulation of target gene expression and activation of key signaling pathways associated with prostate cancer malignancy. PMID: 24823640
    11. CENP-F may serve as valuable molecular marker for predicting prognosis of ESCC patients. data indicate potential benefit of combining ZOL with cisplatin in ESCC; CENP-F expressionmay have therapeutic implications. PMID: 23163484
    12. data suggest that CENPF is frequently overexpressed in HCC and plays a critical role in driving HCC tumorigenesis PMID: 23791740
    13. Data suggest that ASUN promotes perinuclear enrichment of dynein at G2/M that facilitates BICD2- and CENP-F-mediated anchoring of dynein to nuclear pore complexes. PMID: 23097494
    14. Coincidently amplified CDK13, GMNN, and CENPF genes can play a role as common cancer-driver genes in human cancers. PMID: 22912832
    15. Rab5 forms a complex with a subset of CENP-F in mitotic cells and regulates the kinetics of release of CENP-F from the nuclear envelope and its accumulation on kinetochores. PMID: 21987812
    16. Centromere protein F and survivin are malignant behaviour markers for colorectal gastrointestinal stromal tumours. PMID: 21613637
    17. Data identified Cenp-F as a potential new molecular target for NBPs in tumour cells. PMID: 20015195
    18. Cenp-F plays a role in organization of interphase chromatin through association and possibly regulation of DNA-PK. PMID: 20978035
    19. Data suggest that CENP-F protein is a valuable marker of nasopharyngeal carcinoma progression, and CENP-F expression is associated with poor overall survival of patients. PMID: 20828406
    20. Mitosin did not predict patient survival in this series of cutaneous melanomas. PMID: 20398247
    21. These results uncover a novel role of CENP-F in regulation of epigenetic modification on histone H3. PMID: 20213041
    22. Data show that the post-anaphase, KEN-box-dependent degradation of Cenp-F requires it to be farnesylated, a post-translational modification usually linked to membrane association. PMID: 20053638
    23. Data suggest that farnesylation of Cenp-F is required not only for its localisation to the nuclear envelope and kinetochores but also for timely progression through G2/M and its degradation after mitosis. PMID: 12154071
    24. Data show that mitosin associates preferentially with kinetochores of unaligned chromosomes. PMID: 12974617
    25. CENP-F expression presents a theoretical advantage for the analysis of the precise cell cycle of G2 to M cells, compared to Ki-67. PMID: 14720137
    26. Results suggest that mitosin is a negative regulator of ATF4 in interphase through direct interaction. PMID: 15677469
    27. Mitosin is therefore essential for full chromosome alignment, possibly by promoting proper kinetochore attachments through modulating CENP-E and dynein functions PMID: 15870278
    28. In addition to regulating kinetochore-microtubule interactions, Cenp-F might be required to protect centromeric cohesion prior to anaphase commitment. PMID: 16219694
    29. Data show that the absence of nuclear CENP-F does not affect cell cycle progression in S and G2, and that CENP-F is crucial for efficient assembly of a stable microtubule-kinetochore interface. PMID: 16252009
    30. REVIEW: involvement in mitotic control, microtubule dynamics, transcriptional regulation, and muscle cell differentiation. PMID: 16456711
    31. CENP-F is a novel microtubule-binding protein that possesses two microtubule-binding domains at opposite ends of the molecule. The C-terminal microtubule-binding domain was found to stimulate microtubule polymerization in vitro. PMID: 16601978
    32. CENP-F upregulation was significantly associated breast cancer PMID: 17205517
    33. Ndel1, Nde1, and Lis1 localize to kinetochores in a Cenp-F-dependent manner. PMID: 17600710
    34. high expression levels of the CENP-F appeared to be the molecular background of higher proliferative activity, and they were correlated with high SUV (standardized uptake value) in breast cancer PMID: 19102762

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  • 相关疾病：
    Stromme syndrome (STROMS)
  • 亚细胞定位：
    Cytoplasm, perinuclear region. Nucleus matrix. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Note=Relocalizes to the kinetochore/centromere (coronal surface of the outer plate) and the spindle during mitosis. Observed in nucleus during interphase but not in the nucleolus. At metaphase becomes localized to areas including kinetochore and mitotic apparatus as well as cytoplasm. By telophase, is concentrated within the intracellular bridge at either side of the mid-body.
  • 蛋白家族：
    Centromere protein F family
  • 数据库链接：

    HGNC: 1857

    OMIM: 243605

    KEGG: hsa:1063

    STRING: 9606.ENSP00000355922

    UniGene: Hs.497741