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CENPJ Antibody

  • 货号：
    CSB-PA127200
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA127200(CENPJ Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
  • 其他：

产品详情

  • Uniprot No.：
    Q9HC77
  • 基因名：
    CENPJ
  • 别名：
    BM032 antibody; CENP-J antibody; CENPJ antibody; CENPJ_HUMAN antibody; Centromere protein J antibody; Centrosomal P4.1-associated protein antibody; CPAP antibody; LAG-3-associated protein antibody; LAP antibody; LIP1 antibody; LYST interacting protein LIP1 antibody; LYST interacting protein LIP7 antibody; LYST-interacting protein 1 antibody; MCPH6 antibody; Sas 4 antibody; SASS4 antibody; SCKL4 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Fusion protein of Human CENPJ
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:1000-1:2000
    IHC 1:25-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Plays an important role in cell division and centrosome function by participating in centriole duplication. Inhibits microtubule nucleation from the centrosome. Involved in the regulation of slow processive growth of centriolar microtubules. Acts as microtubule plus-end tracking protein that stabilizes centriolar microtubules and inhibits microtubule polymerization and extension from the distal ends of centrioles. Required for centriole elongation and for STIL-mediated centriole amplification. Required for the recruitment of CEP295 to the proximal end of new-born centrioles at the centriolar microtubule wall during early S phase in a PLK4-dependent manner. May be involved in the control of centriolar-microtubule growth by acting as a regulator of tubulin release.
  • 基因功能参考文献：
    1. CPAP regulates delivery of its bound beta-tubulin to define the size of microtubule-based cellular structures using a "clutch-like" mechanism. PMID: 27306797
    2. Data suggest that alcohol/ethanol exposure diminishes pool of proliferative neurons (Neuro2a cell line) through disordering of spindle orientation and promotion of asymmetric cell division; these neuronal abnormalities appear to be due to reduced CENPJ protein expression level. PMID: 29778912
    3. CPAP-S467D protein has a low affinity for microtubule binding but a high affinity for pericentriolar material proteins. PMID: 26997271
    4. CPAP promotes timely cilium disassembly to maintain neural progenitor pool. CPAP mutation causes Seckel syndrome with microcephaly. PMID: 26929011
    5. Data suggest that the single G-box domain (that appears to fold into 14-20 antiparallel beta-strands) of CENPJ has stable but dynamic structure; CRAP forms multimers (in solution and in crystals) of elongated fibrils similar to amyloid fibrils. [REVIEW] PMID: 26517891
    6. Centrobin plays a role in the stability and centriole elongation function of CPAP and limits the centriole length. PMID: 25616662
    7. studies provide the first structural insight into how the malfunction of centriole proteins results in human disease and also reveal that the CPAP-STIL interaction constitutes a conserved key step in centriole biogenesis PMID: 24052813
    8. The results showed a human-specific hypomethylation in the 5' UTR of CENPJ in the brain, where methylation levels among humans are only about one-third of those found among nonhuman primates. PMID: 24288161
    9. Centrobin-CPAP interaction is critical for the recruitment of CPAP to procentrioles to promote the elongation of daughter centrioles and for the persistence of CPAP on preexisting mother centrioles. PMID: 24700465
    10. CPAP depletion results in asymmetric spindle poles with uneven distribution of pericentriolar material. PMID: 24491538
    11. Sas-4 acts as a vehicle to tether PCM complexes to centrioles independent of its well-known role in centriole duplication PMID: 24385583
    12. CEP120 associates with SPICE1 and CPAP, and depletion of any of these proteins results in short procentrioles. Furthermore, CEP120 or CPAP overexpression results in excessive centriole elongation, a process dependent on CEP120, SPICE1, and CPAP. PMID: 23810536
    13. SUMOylated CPAP could synergistically increase the HBx-induced NF-kappaB activity PMID: 23369793
    14. CEP120 is a CPAP-interacting protein that positively regulates centriole elongation. PMID: 23857771
    15. s propose that CEP135 directly connects the central hub protein, hSAS-6, to the outer microtubules, and suggest that this interaction stabilizes the proper cartwheel structure for further CPAP-mediated centriole elongation. PMID: 23511974
    16. CPAP degradation and function is controlled by the poly(ADP-ribose) polymerase tankyrase 1. PMID: 22699936
    17. STIL and CPAP are essential for centriole formation and for proper spindle position. PMID: 22100914
    18. Results suggest that Cep152 recruits Plk4 and CPAP to the centrosome to ensure a faithful centrosome duplication process. PMID: 21059844
    19. Data establishes that mutation of CENPJ can lead to Seckel syndrome and calls for further investigation of the role played by other microcephaly related genes in the pathogenesis of PD. PMID: 20522431
    20. Results identify centrosomal P4.1-associated protein (CPAP), a human homologue of SAS-4, as a substrate of PLK2 whose activity oscillates during the cell cycle. PMID: 20531387
    21. cell cycle-regulated phosphorylation orchestrates the dynamics of CPAP molecular interaction and centrosome splitting to ensure genomic stability in cell division PMID: 19889632
    22. CPAP was found to augment Stat5-mediated transcription PMID: 12198240
    23. CPAP carries a novel microtubule-destabilizing motif that not only inhibits microtubule nucleation from the centrosome but also depolymerizes taxol-stabilized microtubules. PMID: 15047868
    24. CPAP functions as a coactivator of NF-kappaB-mediated transcription PMID: 15687488
    25. Mutations in CENPJ gene is associated with autosomal recessive primary microcephaly PMID: 15793586
    26. Together, our results reveal a structural role for CPAP to maintain centrosome integrity and normal spindle morphology during cell division. PMID: 16316625
    27. In summary, our results show a direct interaction between CPAP and 14-3-3, and this interaction appears to be phosphorylation and cell cycle dependent. PMID: 16516142
    28. discuss CENPJ, which similarly exhibits higher rate of protein evolution in primates as compared to rodents and carnivores PMID: 16631324
    29. High levels of LIP1 were found in serum and synovial fluid of rheumatoid arthritis patients, providing evidence for a cytokine-like role. PMID: 18162190
    30. Mutations in this conserved sequence also eliminate d-SAS-4's microtubule-destabilizing activity, suggesting that d-SAS-4 and CPAP may play similar roles within cells. PMID: 18586240
    31. the PN2-3 fragment of CPAP as a protein with an unprecedented tubulin sequestering mechanism distinct from that of stathmin family proteins. PMID: 19131341
    32. Results suggest that CPAP and CP110 play antagonistic roles in determining the extent of tubulin addition during centriole elongation, thereby controlling the length of newly formed centrioles. PMID: 19481458
    33. Data show that the CPAP is required for centrosome duplication in cycling human cells, and that CPAP overexpression results in the formation of abnormally long centrioles. PMID: 19481460
    34. Results suggest that CPAP is a new regulator of centriole length and its intrinsic tubulin-dimer binding activity is required for procentriole elongation. PMID: 19503075
    35. Identifies CENPJ as LYST-interacting proteins LIP1 and LIP7, which interact with the lysosomal trafficking regulator (LYST) protein. PMID: 11984006

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  • 相关疾病：
    Microcephaly 6, primary, autosomal recessive (MCPH6); Seckel syndrome 4 (SCKL4)
  • 亚细胞定位：
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole.
  • 蛋白家族：
    TCP10 family
  • 数据库链接：

    HGNC: 17272

    OMIM: 608393

    KEGG: hsa:55835

    STRING: 9606.ENSP00000371308

    UniGene: Hs.513379