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CHAF1A Antibody

  • 货号：
    CSB-PA943110
  • 规格：
    ￥2024
  • 图片：
    • Western blot analysis of extracts from K562 cells and A549 cells, using CAF1A antibody.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) CHAF1A Polyclonal antibody
  • Uniprot No.：
    Q13111
  • 基因名：
    CHAF1A
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthesized peptide derived from internal of Human CAF1A.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC,IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:3000
    IHC 1:50-1:200
    IF 1:100-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Core component of the CAF-1 complex, a complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci.
  • 基因功能参考文献：
    1. CHAF1A and PCNA are highly expressed in cervical squamous cell carcinoma and associated with the malignancy PMID: 29382432
    2. Recent work has identified the chromatin assembly factor complex CAF-1 as a potent barrier to cellular reprogramming. In addition, CAF-1 has been implicated in the reversion of pluripotent cells to a totipotent-like state and in various lineage conversion paradigms, suggesting that modulation of CAF-1 levels may endow cells with a developmentally more plastic state. PMID: 28692904
    3. Chromatin reassembly during double-strand break repair was dependent on the HIRA histone chaperone that is specific to the replication-independent histone variant H3.3 and on CAF-1 that is specific to the replication-dependent canonical histones H3.1/H3.2. PMID: 27269284
    4. Data show that the high expression of chromatin assembly factor 1, subunit A (p150) (CHAF1A) promotes cell proliferation and inhibits cell apoptosis, suggesting that CHAF1A may be developed as a prognosis biomarker and potential therapeutic target of epithelial ovarian cancer (EOC). PMID: 28286267
    5. CHAF1A inhibits NEIL1 initiated repair in vitro Subsequent restoration of the chaperone-BER complex in cell, presumably after completion of repair, suggests that histone chaperones sequester the repair complex for oxidized bases in non-replicating chromatin, and allow repair when oxidized bases are induced in the genome. PMID: 27794043
    6. Loss of CAF1 is associated with increased motility and invasive phenotypes seen in transformed cells. PMID: 27872192
    7. These data suggest that CAF-1- and ASF1A-H3-H4-dependent deposition of the histone (H3-H4)2 tetramers is compatible with MMR and protects the discontinuous daughter strand from unnecessary degradation by MMR machinery. PMID: 26945061
    8. Novel functions for a separable domain of the p150 protein, regulating protein and DNA interactions at the nucleolus. PMID: 25057015
    9. The frequency of positive CHAF1A staining in primary tumor mucosa (45.8% of 203 samples) was significantly elevated compared to normal mucosa (18.7% of samples). Increased expression was associated with cancer stage, tumor invasion and histological grade. PMID: 24845563
    10. Histone chaperone CHAF1A inhibits differentiation and promotes aggressive neuroblastoma. PMID: 24335960
    11. CAF1 was hijacked by IE2 to facilitate the replication of the HCMV genome, suggesting chromatin assembly plays an important role in herpesviral DNA synthesis, but also provide a model of the virus-induced chromatin instability through CAF1. PMID: 21445097
    12. CAF-I-dependent control of degradation of the discontinuous strands during mismatch repair. PMID: 21282622
    13. these findings suggest an expanded role of p150 as a SUMO2/3-interacting factor, and raise the intriguing possibility that p150 plays a role in promoting delivery of SUMO2/3 or SUMO2/3-modified proteins (or both) on chromatin fibers during replication. PMID: 19919826
    14. p150 was efficiently phosphorylated by Cdc7-Dbf4 kinase. PMID: 16826239
    15. ACF generates the characteristic 50- to 60-base-pair internucleosomal spacing in silent chromatin by kinetically discriminating against shorter linker DNAs PMID: 17099699
    16. rs9352 of CHAF1A was repeatedly includes (78% of the 200 bootstrap samples) in the best set of SNPs. CHAF1A may contribute to gliomagenesis. PMID: 18559551
    17. p150 directly activates transcription, independently of its histone deposition function. PMID: 19324875
    18. Data show that human CAF-1 (p150) contains two PCNA interaction peptides, and suggest that these peptides are part of the mechanism that enables CAF-1 to function behind replication forks without interfering with other PCNA-mediated processes. PMID: 19822659
    19. Altered mRNA expression is associated with prostate cancer recurrence. PMID: 15067324

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  • 亚细胞定位：
    Nucleus. Note=DNA replication foci.
  • 蛋白家族：
    CHAF1A family
  • 数据库链接：

    HGNC: 1910

    OMIM: 601246

    KEGG: hsa:10036

    STRING: 9606.ENSP00000301280

    UniGene: Hs.79018