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CHAF1B Antibody

  • 中文名称：
    CHAF1B兔多克隆抗体
  • 货号：
    CSB-PA060227
  • 规格：
    ￥880
  • 其他：

产品详情

  • Uniprot No.：
    Q13112
  • 基因名：
    CHAF1B
  • 别名：
    CAF 1 subunit B antibody; CAF 1A antibody; CAF I 60 kDa subunit antibody; CAF IP60 antibody; CAF-1 antibody; CAF-1 subunit B antibody; CAF-I 60 kDa subunit antibody; CAF-I p60 antibody; CAF1 antibody; CAF1A antibody; CAF1B_HUMAN antibody; CAF1P60 antibody; CHAF 1B antibody; CHAF1B antibody; Chromatin assembly factor 1 subunit B antibody; Chromatin assembly factor I antibody; Chromatin assembly factor I p60 subunit antibody; Human chromatin assembly factor-I p60 subunit antibody; M phase phosphoprotein 7 antibody; M-phase phosphoprotein 7 antibody; MPHOSPH7 antibody; MPP 7 antibody; MPP7 antibody; p60 subunit antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthesized peptide derived from the Internal region of Human CAF-1 p60.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, IHC, IF, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:100-1:300
    IF 1:200-1:1000
    ELISA 1:20000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.
  • 基因功能参考文献：
    1. The volumes of the tumors were similar between those injected with the empty vector and control, but were significantly smaller in the knockdown models, suggesting that the knockdown of the CHAF1B gene inhibited tumor growth. H&E staining revealed that tumors were developed in mice in all groups. PMID: 29767268
    2. All the evaluated malignant tumors showed CAF-1 p60 overexpression, confirming the emerging role of CAF-1 p60 as a new proliferation and prognostic marker for cancer. PMID: 23109837
    3. The levels of the EDNRB, HJURP and p60/CAF-1 proteins were strongly associated with overall survival in high-grade gliomas patients (p<0.001, p<0.001 and p=0.002, respectively), whereas the one of PDLI4 was not (P=0.11). PMID: 24039914
    4. Overexpression of CAF-1/p60, on histological and/or cytological samples, characterizes malignant salivary gland tumours with aggressive behaviour PMID: 21109952
    5. CAF-1 is a proliferation marker in various malignant tumours with prognostic value in renal, endometrial and cervical carcinomas, which supports the value of CAF-1 as a clinical marker of cancer progression. PMID: 21083601
    6. Chromatin Assembly Factor-1/p60 may have a role in progression and metastasis of melanoma PMID: 20178651
    7. model is proposed in which the synergism between hAsf1 and CAF-1 for nucleosome formation during DNA repair is achieved through a transient physical interaction allowing histone delivery from Asf1 to CAF-1 [asf1(anti-silencing function 1)] PMID: 11897662
    8. These data identify chromatin assembly factor 1 as an essential factor not only for S-phase-specific chromatin assembly but also for proliferating cell viability. PMID: 15024074
    9. Downregulated protein level of CAF-1 p60 in adult brain of patients with Down syndrome (DS) in contrast to Alzheimer's disease indicates that it can be considered specific for changes in DS. PMID: 15068244
    10. The N- & C-terminal regions of ASF1a and ASF1b determine the different affinities of these two proteins for HIRA, by contacting regions outside the HIRA B domain. CAF-1 p60 also uses B domain-like motifs for binding to ASF1a, thereby competing with HIRA. PMID: 16980972
    11. overexpression of CAF-1/p60 characterized prostatic cancers with a worse prognosis PMID: 19309489
    12. The histone H3K9 methyltransferase SetDB1 associates with the specific heterochromatin protein 1alpha (HP1alpha)-chromatin assembly factor 1 (CAF1) chaperone complex. PMID: 19498464

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Note=DNA replication foci. Cytoplasmic in M phase.
  • 蛋白家族：
    WD repeat HIR1 family
  • 数据库链接：

    HGNC: 1911

    OMIM: 601245

    KEGG: hsa:8208

    STRING: 9606.ENSP00000315700

    UniGene: Hs.75238