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CHD1 Antibody

  • 货号：
    CSB-PA005325ESR2HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA005325ESR2HU at dilution of 1:100
    • Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA005325ESR2HU at dilution of 1:100
    • Chromatin Immunoprecipitation Hela (1.1*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4μg anti-CDH1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers (CSB-PP005325HU) against the RPL30 promoter.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) CHD1 Polyclonal antibody
  • Uniprot No.：
    O14646
  • 基因名：
  • 别名：
    ATP dependent helicase CHD 1 antibody; ATP-dependent helicase CHD1 antibody; CHD 1 antibody; CHD-1 antibody; Chd1 antibody; CHD1_HUMAN antibody; Chromodomain helicase DNA binding protein 1 antibody; Chromodomain-helicase-DNA-binding protein 1 antibody; DKFZp686E2337 antibody; OTTHUMP00000222625 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Chromodomain-helicase-DNA-binding protein 1 protein (1501-1710AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, ChIP
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. Regulates polymerase II transcription. Also required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. Regulates negatively DNA replication. Not only involved in transcription-related chromatin-remodeling, but also required to maintain a specific chromatin configuration across the genome. Is also associated with histone deacetylase (HDAC) activity. Required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. Functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3. Required for maintaining open chromatin and pluripotency in embryonic stem cells.
  • 基因功能参考文献：
    1. CHD1 loss is associated with an increased sensitivity to PARP inhibition and anti-cancer drugs that induce DNA intercross-strand links in prostate tumors. PMID: 28383660
    2. Increased CHD1L protein expression was significantly associated with poor overall survival in pancreatic cancer patients. PMID: 28646284
    3. CHD1 facilitates substrate handover from XPC to the downstream TFIIH (transcription factor IIH). PMID: 29018037
    4. CHD1 is required for the induction of osteoblast-specific gene expression, extracellular-matrix mineralization and ectopic bone formation in vivo. Genome-wide occupancy analyses revealed increased CHD1 occupancy around the transcriptional start site of differentiation-activated genes. PMID: 28475736
    5. examined the role of CHD1 in DNA double-strand break (DSB) repair in prostate cancer cells; findings show that CHD1 is required for the recruitment of CtIP to chromatin and subsequent end resection during DNA DSB repair; data support a role for CHD1 in opening the chromatin around the DSB to facilitate the recruitment of homologous recombination (HR) proteins PMID: 27596623
    6. In PTEN-deficient prostate and breast cancers, CHD1 depletion profoundly and specifically suppressed cell proliferation, cell survival and tumorigenic potential. PMID: 28166537
    7. The s have identified an additional conserved domain C-terminal to the SANT-SLIDE domain and determined its structure by multidimensional heteronuclear NMR spectroscopy. They have termed this domain the CHD1 helical C-terminal (CHCT) domain as it is comprised of five alpha-helices arranged in a variant helical bundle topology. PMID: 27591891
    8. These results indicate that CHD1 is a positive regulator of influenza virus multiplication and suggest a role for chromatin remodeling in the control of the influenza virus life cycle. PMID: 26792750
    9. These data link the assembly of methylated KDM1A and CHD1 with AR-dependent transcription and genomic translocations, thereby providing mechanistic insight into the formation of TMPRSS2-ERG gene fusions during prostate-tumor evolution. PMID: 26751641
    10. The double chromodomains of CHD1 adopt an 'open pocket' to interact with the free N-terminal amine of H3K4, and the open pocket permits the NS1 mimic to bind in a distinct conformation. PMID: 24853335
    11. identify coordinate loss of MAP3K7 and CHD1 as a unique driver of aggressive prostate cancer development PMID: 25770290
    12. CHD1 and CHD2 act as positive regulators of HIV-1 gene expression. PMID: 25297984
    13. results demonstrate the ability of confocal microscopy and FISH to identify the cell-to-cell differences in common gene fusions such as TMPRSS2-ERG that may arise independently within the same tumor focus PMID: 25175909
    14. Data indicate that chromodomain-helicase-DNA-binding protein CHD1, neoplasm protein GREB1 and karyopherin alpha 2 protein KPNA2 as critical mediators of miR-26a and miR-26b elicited cell growth. PMID: 24735615
    15. CHD1 is the 5q21 tumor suppressor gene in prostate cancer PMID: 23492366
    16. findings suggest that CHD1 deletion may underlie cell invasiveness in a subset of prostate cancers, and indicate a possible novel role of altered chromatin remodeling in prostate tumorigenesis PMID: 22179824
    17. findings collectively suggest that distinct CHD1-associated alterations of genomic structure evolve during and are required for the development of prostate cancer PMID: 22139082
    18. CHD1, was the most "dietary sensitive" genes, as methylation of their promoters was associated with intakes of at least two out of the eight dietary methyl factors examined. PMID: 22048254
    19. that hPaf1/PD2 in association with MLL1 regulates methylation of H3K4 residues, as well as interacts and regulates nuclear shuttling of chromatin remodeling protein CHD1, facilitating its function in pancreatic cancer cells PMID: 22046413
    20. Mediator coactivator complex, which controls PIC assembly, is also necessary for CHD1 recruitment PMID: 21979373
    21. associates with NCoR and histone deacetylase as well as with RNA splicing proteins PMID: 12890497
    22. yeast and human CHD1 have diverged in their ability to discriminate covalently modified histones and link histone modification-recognition and non-covalent chromatin remodeling activities within a single human protein PMID: 16263726
    23. the structure of the tandem arrangement of the human CHD1 chromodomains, and its interactions with histone tails PMID: 16372014
    24. analysis of chromodomains in human and fungal Chd1 PMID: 17098252
    25. overexpression of CHD1L could sustain tumor cell survival by preventing Nur77-mediated apoptosis PMID: 19441106

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    SNF2/RAD54 helicase family
  • 组织特异性：
    Expressed in many tissues including in the brain, where the highest level of expression is found in the cerebellum and basal ganglia.
  • 数据库链接：

    HGNC: 1915

    OMIM: 602118

    KEGG: hsa:1105

    STRING: 9606.ENSP00000284049

    UniGene: Hs.643465