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CHRNB3 Antibody

  • 货号：
    CSB-PA005397LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Mouse liver tissue
      All lanes: CHRNB3 antibody at 5.1μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 53 kDa
      Observed band size: 53 kDa
    • IHC image of CSB-PA005397LA01HU diluted at 1:400 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA005397LA01HU diluted at 1:400 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA005397LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) CHRNB3 Polyclonal antibody
  • Uniprot No.：
    Q05901
  • 基因名：
    CHRNB3
  • 别名：
    CHRNB3 antibody; Neuronal acetylcholine receptor subunit beta-3 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse
  • 免疫原：
    Recombinant Human Neuronal acetylcholine receptor subunit beta-3 protein (67-218AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，CHRNB3 Antibody (CSB-PA005397LA01HU)，的标记方式是Non-conjugated。对于CHRNB3 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA005397LB01HU CHRNB3 Antibody, HRP conjugated ELISA
    FITC CSB-PA005397LC01HU CHRNB3 Antibody, FITC conjugated
    Biotin CSB-PA005397LD01HU CHRNB3 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • 基因功能参考文献：
    1. To gain a better understanding of the pathological processes underlying ND and ND-related behaviors and to promote the development of effective smoking cessation therapies, we here present the most recent studies concerning the genetic effects of the CHRNB3-CHRNA6 gene cluster in ND. PMID: 27327258
    2. More low frequency variants in the CHRNA6/CHRNB3 gene region were observed in cases compared to controls. PMID: 27085880
    3. Data provide evidence that the protective genetic variant at rs6474413 identified in human genetic studies reduces gene expression and that decreased beta3 gene expression in mice reduces nicotine intake PMID: 26318101
    4. This patient's chromosomal abnormality affected only one gene that is highly expressed in the brainstem and brain, involved in neurotransmission, and could be related to her Duane retraction syndrome. PMID: 24001015
    5. the CHRNB3-A6 locus contains multiple variants affecting risk for vulnerability to cocaine and nicotine dependence as well as bipolar disorder, suggesting that they have pleiotropic effects. PMID: 24675634
    6. Polymorphism in CHRNB3 gene is associated with esophageal adenocarcinoma. PMID: 25823894
    7. The common variant rs13273442 in the CHRNB3-CHNRA6 region is associated significantly with nicotine dependence in European Americans and African Americans. PMID: 24401102
    8. CHRNB3 c.-57A>G alteration affects the promoter activity and is associated with Parkinson's disease (PD), and smoking in PD patients. PMID: 24731518
    9. this study provides convincing evidence for a role of CHRNB3 in Nicotine Dependence. PMID: 23319001
    10. these results demonstrate that the combined effect of rs6474412-C/T polymorphism in smoking-related CHRNB3-CHRNA6 region gene and smoking behavior may not confer risk to psoriasis vulgaris (PV), but may have impact on PV severity in Chinese Han population. PMID: 24792900
    11. Results demonstrate the association between SNPs in CHRNB3 nicotinic acetylcholine receptor genes and the risk of smoking in ADHD PMID: 23899432
    12. The results indicate the genetic locus for the CHRNB3 gene is strongly associated with nicotine dependence. PMID: 22524403
    13. beta3 subunit coexpression promotes function of alpha6*-nAChR PMID: 22315221
    14. Variants in CHRNB3/CHRNA6 are independently associated with bipolar disorder. PMID: 21191315
    15. The GG genotype of SNP rs13261190 in the CHRNB3 was associated with increased novelty seeking in alcohol-dependent individuals. PMID: 21606657
    16. CHRNB3 and CHRNA6 polymorphisms are associated with smoking behavior and lung cancer susceptibility in Chinese Han population. PMID: 21831805
    17. Three single nucleotide polymorphisms (SNPs) in CHRNA6 and one SNP in CHRNB3 are associated with a composite of alcohol phenotypes. PMID: 19500157
    18. CHRNB3-CHRNA6 and CYP2A6 sequence variants affect smoking behavior PMID: 20418888
    19. CHRNB3 is associated with subjective responses to tobacco. PMID: 18055561
    20. Together these results further implicate the region downstream of CHRNA6 and the region upstream of CHRNB3 in risk of nicotine dependence. PMID: 18704094

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  • 亚细胞定位：
    Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Ligand-gated ion channel (TC 1.A.9) family, Acetylcholine receptor (TC 1.A.9.1) subfamily, Beta-3/CHRNB3 sub-subfamily
  • 数据库链接：

    HGNC: 1963

    OMIM: 118508

    KEGG: hsa:1142

    STRING: 9606.ENSP00000289957

    UniGene: Hs.654576