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CLK1 Antibody

  • 货号：
    CSB-PA556042
  • 规格：
    ￥2024
  • 图片：
    • Western blot analysis of extracts from 293 cells, using CLK1 antibody.
    • Immunofluorescence analysis of HUVEC cells, using CLK1 antibody.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) CLK1 Polyclonal antibody
  • Uniprot No.：
    P49759
  • 基因名：
    CLK1
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthesized peptide derived from internal of Human CLK1.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:3000
    IF 1:100-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates: SRSF1, SRSF3 and PTPN1. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells and adenovirus E1A pre-mRNA.
  • 基因功能参考文献：
    1. We now show that the ability of SRPK1 to mobilize SRSF1 from speckles to the nucleoplasm is dependent on active CLK1. Diffusion from speckles is promoted by the formation of an SRPK1-CLK1 complex that facilitates dissociation of SRSF1 from CLK1 and enhances the phosphorylation of several serine-proline dipeptides in this SR protein PMID: 29335301
    2. Data suggest that CLK1 activity in both transformed and normal cells is carefully regulated via CLK1 alternative splicing through both exon 4 skipping and intron 4 retention. CLK1 autoregulates by favoring the expression of truncated isoforms, CLK1T1 and CLKT2 in environmental stress. PMID: 29802995
    3. These results thus reveal a large program of CLK1-regulated periodic alternative splicing intimately associated with cell cycle control. PMID: 27015110
    4. Global CLK-dependent exon recognition and conjoined gene formation revealed with a novel small molecule inhibitor has been described. PMID: 28232751
    5. that mitochondrial Clk1 regulated chemoresistance in glioma cells through AMPK/mTOR/HIF-1alpha mediated glycolysis pathway PMID: 28581641
    6. SRPK1 interacts with an RS-like domain in the N terminus of CLK1 to facilitate the release of phosphorylated SR proteins, which then promotes efficient splice-site recognition and subsequent spliceosome assembly. PMID: 27397683
    7. Clk1, Clk2 and Clk4 prevent chromatin breakage by regulating the Aurora B-dependent abscission checkpoint. PMID: 27126587
    8. removal of the N-terminus or dilution of CLK1 induces monomer formation and reverses this specificity. CLK1 self-association also occurs in the nucleus PMID: 26443864
    9. Nuclear CLK-1 mediates a retrograde signalling pathway that is conserved from Caenorhabditis elegans to humans and is responsive to mitochondrial reactive oxygen species, thus acting as a barometer of oxidative metabolism. PMID: 25961505
    10. Data suggest that proline phosphorylation by CLK1/CDC-like kinase 1 (but not by SRPK1/serine/arginine-rich splicing factor kinase 1) regulates conformation and alternative splicing function of SFRS1 (serine/arginine-rich splicing factor 1). PMID: 25529026
    11. findings suggest that CLK1-dependent hyperphosphorylation is the result of a general mechanism in which the N-terminus acts as a bridge connecting the kinase domain and the RS domain of the SR protein. PMID: 24869919
    12. The data establish a new view of SRSF1 protein regulation in which SRPK1 and CLK1 partition activities based on Ser-Pro versus Arg-Ser placement rather than on N- and C-terminal preferences along the RS domain. PMID: 23707382
    13. CLK1 Increases While CLK2 Decreases HIV-1 Gene Expression. PMID: 21682887
    14. Clk/Sty is found in the nucleus of several different cell types and is a factor directly involved in splicing control PMID: 12773558
    15. ASF/SF2 is phosphorylated by SRPK1 and Clk/Sty PMID: 16223727
    16. Cdc2-like kinases and DNA topoisomerase I regulate alternative splicing of tissue factor in human endothelial cells. PMID: 19168442

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, Lammer subfamily
  • 组织特异性：
    Endothelial cells.
  • 数据库链接：

    HGNC: 2068

    OMIM: 601951

    KEGG: hsa:1195

    STRING: 9606.ENSP00000394734

    UniGene: Hs.433732