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  4. CLTC Antibody

CLTC Antibody

  • 货号：
    CSB-PA777315
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using CSB-PA777315(CLTC Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
    • The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA777315(CLTC Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
    • Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-2: A172 cells, hela cells, Primary antibody: CSB-PA777315(CLTC Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds
  • 其他：

产品详情

  • Uniprot No.：
    Q00610
  • 基因名：
  • 别名：
    CHC17 antibody; Clathrin heavy chain antibody; Clathrin heavy chain 1 antibody; Clathrin heavy chain 2 antibody; Clathrin heavy chain like 1 antibody; Clathrin heavy chain on chromosome 17 antibody; Clathrin heavy polypeptide antibody; CLH 17 antibody; CLH 22 antibody; CLH-17 antibody; CLH1_HUMAN antibody; CLH17 antibody; CLH22 antibody; CLTC antibody; CLTCL antibody; CLTCL1 antibody; CLTCL2 antibody; CLTD antibody; Hc antibody; KIAA0034 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthetic peptide of Human CLTC
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:1000-1:2000
    WB 1:200-1:1000
    IHC 1:25-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Plays a role in early autophagosome formation.
  • 基因功能参考文献：
    1. Provide biochemical evidence for separate regulation and distinct functional niches for CHC17 and CHC22 in HeLa cells. Furthermore, the greater stability of the CHC22 coat relative to the CHC17 coat may be relevant to its excessive accumulation with GLUT4 during insulin resistance. PMID: 29097553
    2. this study shows that clathrin, including the clathrin population associated to internal vesicles, controls lymphocyte migration by regulating actin rearrangements occurring at the leading age PMID: 27405273
    3. The novel association of several endocytosis-associated proteins has been demonstrated, in particular, of CLTC and SCAMP3 with hepatocellular carcinoma progression. PMID: 27519163
    4. MiR-199a-5p suppresses tumorigenesis by targeting CLTC in hepatocellular carcinoma. PMID: 28261837
    5. SMAP2 immunoprecipitated clathrin and AP-1 through a putative clathrin-binding domain and a CALM-binding domain, and SMAP2 mutants that did not interact with clathrin or AP-1 could not localize to recycling endosomes PMID: 26136365
    6. The CHC:ESR1 interaction is required to sustain estradiol signaling. PMID: 25860340
    7. autophagy contributed to Cx31.1 degradation, and clathrin might be involved in the autophagy of Cx31.1. PMID: 25388970
    8. Data suggest that by binding to both clathrin and F-actin, mammalian actin-binding protein 1 (mAbp1; HIP-55 or SH3P7) is specifically recruited at a late stage of clathrin-coated pits (CCPs) formation, which subsequently recruits dynamin to CCPs. PMID: 25690657
    9. Our data support a model in which nascent Hepatits C Virus particles associate with apoE in the endoplasmic reticulum and exit cells following a clathrin-dependent transendosomal secretory route. PMID: 25631092
    10. These data indicate that clathrin is required for the function of AP-1- and GGA-coated carriers at the trans-Golgi network but may be dispensable for outward traffic en route to the plasma membrane. PMID: 24407285
    11. SNX9 and CHC function in the same molecular pathway for chromosome alignment and segregation, which is dependent on their direct association. PMID: 23861900
    12. clathrin interacts with Rab5 and plays a fundamental role in the entry and intracellular survival of B. abortus via interaction with lipid rafts and actin rearrangement PMID: 23940042
    13. High clathrin heavy chain expression promotes tumor pancreatic growth and angiogenesis through regulation of HIF-1alpha and VEGF signaling PMID: 23228632
    14. This study reveals a novel functional role of clathrin-coated structure in GMR signaling and the oncogenesis of JAK2V617F. PMID: 22935703
    15. The s demonstrate that clathrin promotes clustering of the vaccinia virus actin tail nucleator A36 and host N-WASP, which activates actin nucleation through the Arp2/3 complex. PMID: 22980331
    16. Depletion of clathrin heavy chain (CHC)17, but not the CHC22 clathrin isoform, by ribonucleic acid interference (RNAi) induces centrosome amplification and multipolar spindles. PMID: 22891263
    17. Electron tomography reveals Rab6 is essential to the trafficking of trans-Golgi clathrin and COPI-coated vesicles and the maintenance of Golgi cisternal number PMID: 22335553
    18. These results indicate that Echovirus 7 is internalized by clathrin-mediated endocytosis. PMID: 22496312
    19. Postmitotic Golgi reassembly requires the clathrin heavy chain (CHC). PMID: 21965600
    20. is required for lamellipodium formation PMID: 22010197
    21. Our findings strongly suggest that clathrin and clathrin-mediated signaling pathways are involved in the inflammatory signal transduction of orbital fibroblasts in Graves ophthalmopathy. PMID: 21917865
    22. CHC functions as a built-in molecular brake that ensures a tight control of basal NF-kappaB activation and gene expression in unstimulated cells. PMID: 21364927
    23. Data suggest that clathrin's role in intermicrotubule bridging can be fulfilled not only by trimers but also by dimers, and that the membrane trafficking and mitotic functions of clathrin are independent and separable. PMID: 21362119
    24. The s now report that the integrase domain of Gag-Pol is required for the incorporation of clathrin into HIV-1 virions. PMID: 21289110
    25. Clathrin is recruited to the mitotic spindle by TACC3 and ch-TOG. Together the complex forms inter-microtubule bridges in kinetochore fibres PMID: 21297582
    26. These data indicate that the endocytic uptake of the clostridial glucosylating toxins involves a dynamin-dependent process that is mainly governed by clathrin. PMID: 20498856
    27. Data show in mammalian cells that the heavy chain of clathrin interacts with Atg16L1 and is involved in the formation of Atg16L1-positive early autophagosome precursors. PMID: 20639872
    28. the association between aurora A phosphorylation and spindle apparatus; regulation from aurora A is mediated by CHC in recruiting phospho-TACC3 and subsequently ch-TOG to mitotic spindles. PMID: 20566684
    29. Endocytosis of FcalphaR is clathrin- and dynamin-dependent, but is not regulated by Rab5, and the endocytic motif is not located in the cytoplasmic domain of FcalphaR. PMID: 19859085
    30. neither clathrin nor AP-2 is essential for the internalization of epidermal growth factor PMID: 12960147
    31. Clathrin heavy chain phosphorylation and dephosphorylation are involved in TCR internalization; this is a regulatory mechanism linking TCR signaling to endocytosis. PMID: 15067034
    32. Nuclear CHC is required for the transactivation of p53 target genes and plays a distinct role from clathrin-mediated endocytosis. PMID: 16618797
    33. Syt I plays a pivotal role in mediating cAMP- and Ca(2+)-induced endocytosis of NHE3 (but not in inhibition of activity) through cargo recognition of NHE3 and subsequent recruitment of AP2-clathrin assembly required for membrane endocytosis. PMID: 17307723
    34. Results establish a role for the retromer complex in retrograde transport of the B-subunit of Shiga toxin, and strongly suggest that clathrin and retromer function in consecutive retrograde sorting steps on early endosomes. PMID: 17550971
    35. Nuclear CHC plays a role distinct from clathrin-mediated endocytosis. PMID: 17952123
    36. The clathrin inhibitor phenylarsine oxide prevented SubAB entry and BiP cleavage in SubAB-treated Vero, HeLa and N2A cells demonstrating that SubAB internalization is clathrin-dependent. PMID: 18042253
    37. cytosolic p53 may participate in the regulation of clathrin-mediated endocytosis to control the correct signaling from EGFR PMID: 18363968
    38. The endocytic clathrin machinery can internalize double-membrane vesicles into cells. PMID: 18656476
    39. Endocytosis of shiga toxin (Stx) in Ramos cells requires Syk activity and that Syk is recruited to the uptake site of Stx. PMID: 19289168
    40. Data compared each paralogue in functional assays of endocytosis and mitosis, and find that CHC17 and CHC22 are functionally equivalent. PMID: 19509056
    41. clathrin serving as a regulator of SNX4-dependent transport; upon clathrin release, dynein may bind SNX4 and mediate retrograde movement PMID: 19529763
    42. GAK and CHC cooperated in the same pathway and interacted in mitosis to regulate the formation of a functional spindle. PMID: 19654208
    43. Structural modeling analyses predict that an Asn1288 residue in CHC is crucial for binding to p53. In fact, substitution of this Asn to Ala of CHC diminished its ability to interact with p53, leading to reduced activity to transactivate p53. PMID: 19766654
    44. The assembly efficiency of the various hepatitis delta virus genotypes correlates well with the clathrin heavy chain-binding activity of their HDAg-Ls and coincides with the severity of disease outcome. PMID: 19793827

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  • 亚细胞定位：
    Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Melanosome. Cytoplasm, cytoskeleton, spindle.
  • 蛋白家族：
    Clathrin heavy chain family
  • 数据库链接：

    HGNC: 2092

    OMIM: 118955

    KEGG: hsa:1213

    STRING: 9606.ENSP00000269122

    UniGene: Hs.491351