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  4. COPS5 Antibody

COPS5 Antibody

  • 货号：
    CSB-PA003075
  • 规格：
    ￥880
  • 图片：
    • Western Blot analysis of K562 cells using JAB1 Polyclonal Antibody
    • Western Blot analysis of Jurkat NIH-3T3 K562 293 cells using JAB1 Polyclonal Antibody
  • 其他：

产品详情

  • Uniprot No.：
    Q92905
  • 基因名：
    COPS5
  • 别名：
    38 kDa Mov34 homolog antibody; COP9 (constitutive photomorphogenic) homolog subunit 5 antibody; COP9 constitutive photomorphogenic homolog subunit 5 antibody; COP9 signalosome complex subunit 5 antibody; COP9 signalosome subunit 5 antibody; Cop9 subunit 5 antibody; COPS 5 antibody; cops5 antibody; CSN 5 antibody; CSN5 antibody; CSN5_HUMAN antibody; JAB 1 antibody; Jun activation domain binding protein 1 antibody; Jun activation domain binding protein antibody; Jun activation domain-binding protein 1 antibody; MGC3149 antibody; MOV 34 antibody; MOV34 antibody; MOV34 family; 38-KD member antibody; SGN 5 antibody; SGN5 antibody; Signalosome subunit 5 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthesized peptide derived from the Internal region of Human JAB1.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, IHC, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:100-1:300
    ELISA 1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. Promotes the proteasomal degradation of BRSK2.
  • 基因功能参考文献：
    1. CSN5 directly bound survivin and decreased its ubiquitination to enhance the protein stability of survivin. PMID: 29596838
    2. verexpression of COPS5, through its isopeptidase activity, leads to ubiquitination and proteasome-mediated degradation of NCoR, a key corepressor for ERalpha and tamoxifen-mediated suppression of ERalpha target genes. PMID: 27375289
    3. These findings provide novel insights into molecular mechanism of let-7d and Jab1 in tumor development and progression of breast cancer, and thus let-7d/Jab1 are novel potential therapeutic targets for breast cancer patients. PMID: 29975923
    4. We identified a novel Jab1-Trx axis that is a key cellular process in the pathobiologic characteristics of acute myeloid leukemia (AML-M5). Targeting the ROS/Jab1/Trx pathway could be beneficial in the treatment of AML-M5 PMID: 28270496
    5. These data identified CSN5 as a critical oncoprotein involved in progression of hepatocellular carcinoma (HCC) cells, which could serve as a potential therapeutic target in HCC patients. PMID: 29189991
    6. The s find that UCHL3 regulates COPS5-dependent deneddylation of Cullin1, which is an essential component of SCF(beta-TrCP) complex and associated with SCF(beta-TrCP) activities. The s further demonstrate that UCHL3 upregulates the levels of SCF(beta-TrCP) substrates including IFN-I receptor IFNAR1, which enhances IFN-I mediated signaling pathway and antiviral activity. PMID: 28583475
    7. Collectively, our findings suggest that JAB1 activates the neuronal differentiation ability of CPNE1 through the binding of C2A domain in CPNE1 with MPN domain in JAB1. PMID: 29448099
    8. data suggests that Jab1-mediated phosphorylation of p53 at Thr155 residue mediates nuclear export of p53 PMID: 28539160
    9. CSN5 is contributed to colorectal cancer development by actively driving aberrant WNT signaling through repression of the WNT antagonist DKK1. PMID: 28229932
    10. The results of this study suggest that Jab1 promotes glioma cell proliferation and increased expression of Jab1 in glioma patients may amplify beta-catenin signaling to contribute to glioma cell proliferation. PMID: 27640199
    11. Furthermore, inhibition of COPS5 resulted in an elevation of Akt expression and sensitized SOC cells to Akt inhibitor MK2206. Suppression of COPS5 and Akt offers a potential strategy for the treatment of SOC. PMID: 28919423
    12. The data identified CSN5 as a critical oncoprotein involved in migration and invasion of RCC cells, which could serve as a potential therapeutic target in RCC patients. PMID: 28479251
    13. High JAB1 expression is associated with nasopharyngeal cancer. PMID: 27524414
    14. oxLDL induces JAB1 expression and influences its cellular localization, whereby the p38 MAPK signaling pathway is modified with consequences for inflammation of human MPhi in foam cells and atherosclerotic lesions. PMID: 28173800
    15. We found that increased expression of JAB1 promoted odontogenic differentiation of DPSCs via Wnt/beta-catenin signaling. The role of JAB1 in the odontogenic differentiation of DPSCs was further confirmed by knocking down JAB1. Our findings provide novel insights on odontogenic differentiation of DPSCs. PMID: 26989054
    16. Copine3 binding to ErbB2 increases when Jab1 is overexpressed in SKBr3 breast cancer cells. PI3 kinase and AKT were also activated by Jab1 overexpression.Copine3 and Jab1 binding regulates the ErbB2 signaling pathway. PMID: 26719032
    17. The reverse correlation of Jab1 and Smad4 in PANC-1 cells may be involved in the Pathogenesis of prostate cancer. Jab1 can cause degradation of Smad4 via TGF-beta signal pathway. PMID: 26464677
    18. Jab1/Csn5 expression with concurrent low p57 expression associated with poor overall survival in hepatocellular carcinoma PMID: 26606000
    19. LPA stimulated interaction of CSN5 with HIF1alpha and MIF. Depletion of CSN5 mitigated the association between HIF1alpha and MIF. We suggest HIF1alpha, MIF, and CSN5 form a ternary complex that is necessary to prevent degradation of HIF1alpha under aerobic conditions PMID: 26352431
    20. Integrin beta-1, transforming growth factorbeta1 and nuclear factorkappaB pathways are modified by CSN5 in hepatocllular carcinoma cells. PMID: 26035694
    21. Down regulation of CSN5 may inhibit invasion and arrests cell cycle progression in colorectal cancer via PI3K/AKT/NF-kappaB signal pathway. PMID: 26045788
    22. Knockdown of CSN5 could inhibit proliferation and promote apoptosis of gastric cancer cells. Additionally, suppression of CSN5 expression contributed to the increased expression levels of p53 and Bax. PMID: 26048783
    23. Jab1 expression was increased in endometriosis. PMID: 25666480
    24. CSN5 promotes SIAH-1 degradation in HCT116 and SW480 cells and that this is associated with its deNEDDylase activity. PMID: 24882689
    25. CSN4 silencing decreases CSN5 protein levels and suggest that the CSN4 effects on sGCA1 and p53 proteins are mediated by CSN5. PMID: 24725084
    26. data suggest that Jab1 plays an important role in CAM-DR, which depends on pSer10-p27(Kip1)-mediated subcellular localization of p27(Kip1). PMID: 24170542
    27. A direct interaction of Jab1 with LMP-1, is reported. PMID: 24078030
    28. High JAB1 expression is associated with hepatocellular carcinoma. PMID: 24671224
    29. increased expression in atherosclerotic lesions correlates with atheroprogression PMID: 23636414
    30. Interaction between JAB1 and pre-S2 mutant large surface antigen of hepatitis B virus is facilitated by divalent metal Zn(2+) ion. PMID: 24049181
    31. NEDD8 modification pathway is involved in the proteolysis of IFNR and that JAB1 acts as a positive regulator of IFN responses by stabilizing IFNR through antagonizing the NEDD8 pathway. PMID: 24043623
    32. CSN5 regulates the stability of the inner kinetochore components CENP-T and CENP-W, providing the first direct link between CSN5 and the mitotic apparatus, highlighting the role of CSN5 as a multifunctional cell cycle regulator. PMID: 23926101
    33. results suggest that nuclear JAB1 positively regulates unphosphorylated STAT3 DNA-binding activity through protein-protein interaction in human colon cancer cell line COLO205 PMID: 23911788
    34. findings suggest that Jab1 has an important role in the cellular response to cisplatin and irradiation by regulating DNA damage and repair pathways; observations suggest that Jab1 is a major contributor to the cisplatin, IR and UV radiation resistance of nasopharyngeal carcinoma PMID: 22797071
    35. Jab1 overexpression contributes to pathogenesis of oral squamous cell carcinomas by degradating p27 expression. PMID: 22311264
    36. Studies indicate Jun activation domain-binding protein Jab1 as a substrate for CUL4B E3 ligase. PMID: 23357576
    37. Although the CSN5 active site is catalytically competent and compatible with di-isopeptide binding, the Ins-1 segment obstructs access to its substrate-binding site, and structural rearrangements are necessary for the Nedd8-binding pocket formation. PMID: 23288897
    38. JAB1 expression overcame the inhibition of HIV-1 replication in the presence of peptide and also promoted HIV-1 replication in activated primary CD4(+) T cells through LFA-1. PMID: 22911848
    39. The expression rate of Jab1 protein in laryngeal squamous cell carcinoma is significantly higher than that of normal laryngeal mucosa. There is a negative relationship between Jab1 and p27kiPl protein in laryngeal squamous cell carcinoma. PMID: 22675932
    40. highlight recent advances in studies of the oncogenic role of Jab1/CSN5 in NPC and its potential as a therapeutic target for this cancer PMID: 22867945
    41. study demonstrates that the Src/Stat3 and C/EBP signaling pathways positively regulate the expression of the Jab1 oncogene; results show that Stat3 and LAP2 (C/EBP-beta2) are the two major transcription factors that contribute to Jab1 overexpression that leads to increased proliferation of breast cancer cells PMID: 21689417
    42. An increased level of CSN1 and CSN5 as an important part of the ubiquitin proteasome system (UPS) might be associated with the pathophysiology of preeclampsia. PMID: 22103747
    43. these findings provide a novel regulatory mechanism of BRSK2 through direct interaction with Jab1. PMID: 22609399
    44. The Jab1 may play a vital role in the pathogenesis of both chronic rhinosinusitis and nasal polyposis. PMID: 21466889
    45. role of CSN in the pathogenesis of CRC via regulation of the Wnt/beta-catenin pathway PMID: 22668871
    46. Jab1/CSN5 negatively regulates p27 and plays a role in the pathogenesis of nasopharyngeal carcinoma. PMID: 22350412
    47. overexpression of Jab1, cytoplasmic p27, and pSer10p27 proteins is correlated with poor outcome in patients with glioma PMID: 21837501
    48. results indicated that C10ORF97 functions as a novel tumor suppressor by modulating several key G(1)/S-regulatory proteins by interacting with JAB1 PMID: 21499297
    49. Studies indicate that CSN-mediated deneddylation located to CSN5, and By deneddylation, phosphorylation and deubiquitination the CSN influences DDR outcome which can be successful DNA repair. PMID: 21510940
    50. Bcr-Abl stimulates Jab1 expression via the cooperative interaction of beta-catenin and STAT1 in leukemia cells. PMID: 21935931

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  • 亚细胞定位：
    Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle.
  • 蛋白家族：
    Peptidase M67A family, CSN5 subfamily
  • 数据库链接：

    HGNC: 2240

    OMIM: 604850

    KEGG: hsa:10987

    STRING: 9606.ENSP00000350512

    UniGene: Hs.491912