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DDX21 Antibody

  • 货号：
    CSB-PA006612GA01HU
  • 规格：
    ￥3,900
  • 其他：

产品详情

  • Uniprot No.：
    Q9NR30
  • 基因名：
  • 别名：
    Ddx21 antibody; DDX21_HUMAN antibody; DEAD (Asp-Glu-Ala-Asp) box helicase 21 antibody; DEAD (Asp-Glu-Ala-Asp) box polypeptide 21 antibody; DEAD box protein 21 antibody; EC 3.6.1.- antibody; Gu alpha antibody; Gu protein antibody; Gu-alpha antibody; GUA antibody; GURDB antibody; Nucleolar RNA helicase 2 antibody; Nucleolar RNA helicase Gu antibody; Nucleolar RNA helicase II antibody; RH II/Gu antibody; RH II/GuA antibody; RNA helicase II/Gu alpha antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Human DDX21
  • 免疫原种属：
    Homo sapiens (Human)
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC,IF
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    RNA helicase that acts as a sensor of the transcriptional status of both RNA polymerase (Pol) I and II: promotes ribosomal RNA (rRNA) processing and transcription from polymerase II (Pol II). Binds various RNAs, such as rRNAs, snoRNAs, 7SK and, at lower extent, mRNAs. In the nucleolus, localizes to rDNA locus, where it directly binds rRNAs and snoRNAs, and promotes rRNA transcription, processing and modification. Required for rRNA 2'-O-methylation, possibly by promoting the recruitment of late-acting snoRNAs SNORD56 and SNORD58 with pre-ribosomal complexes. In the nucleoplasm, binds 7SK RNA and is recruited to the promoters of Pol II-transcribed genes: acts by facilitating the release of P-TEFb from inhibitory 7SK snRNP in a manner that is dependent on its helicase activity, thereby promoting transcription of its target genes. Functions as cofactor for JUN-activated transcription: required for phosphorylation of JUN at 'Ser-77'. Can unwind double-stranded RNA (helicase) and can fold or introduce a secondary structure to a single-stranded RNA (foldase). Together with SIRT7, required to prevent R-loop-associated DNA damage and transcription-associated genomic instability: deacetylation by SIRT7 activates the helicase activity, thereby overcoming R-loop-mediated stalling of RNA polymerases. Involved in rRNA processing. May bind to specific miRNA hairpins. Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1.
  • 基因功能参考文献：
    1. can drastically reduce DDX21's affinity for quadruplex, indicating that the recognition of quadruplex and specificity for telomeric repeat containing RNA quadruplex is mediated by interactions with the 2'OH of loop nucleotides PMID: 29906500
    2. Knockdown of SIRT7 leads to the same phenotype as depletion of DDX21 (i.e., increased formation of R loops and DNA double-strand breaks), indicating that SIRT7 and DDX21 cooperate to prevent R-loop accumulation, thus safeguarding genome integrity. PMID: 28790157
    3. DDX21 can suppress the expression of proteins with G4 qudruplexes in the 3 UTR of its mRNA. PMID: 28472472
    4. Results report the biogenesis and function of a box H/ACA snoRNA-ended sno-lncRNA, referred to SLERT (snoRNA-ended lncRNA enhances pre-ribosomal RNA transcription). SLERT is different from Prader-Willi Syndrome (PWS) sno-lncRNAs and plays a crucial role in rRNA biogenesis by dislodging a previously unknown clamp of DDX21 ring-shaped arrangements on Pol I complexes, thereby liberating Pol I for active rRNA transcription. PMID: 28475895
    5. In dengue virus infected cells, DDX21 translocates from nucleus to cytoplasm to active the innate immune response and thus inhibits DENV replication in the early stages of infection. PMID: 27033607
    6. DDX21 expression in breast cancer cells can promote AP-1 activity and rRNA processing, and thus, promote tumorigenesis by two independent mechanisms. PMID: 25260534
    7. Identification of several late-acting snoRNAs that bind pre-40S particles in human cells and show that their association and function in pre-40S complexes is regulated by the RNA helicase DDX21. PMID: 25477391
    8. results uncover the multifaceted role of DDX21 in multiple steps of ribosome biogenesis, and provide evidence implicating a mammalian RNA helicase in RNA modification and Pol II elongation control PMID: 25470060
    9. As sequential interaction of PB1 and NS1 with DDX21 leads to temporal regulation of viral gene expression, influenza A virus likely uses the DDX21-NS1 interaction not only to overcome restriction, but also to regulate the viral life cycle. PMID: 24721576
    10. Data indicate that DDX21, a nucleolar protein, was confirmed to associate with SET8. PMID: 23419719
    11. RNA helicase II/Gualpha silencing inhibits mammalian ribosomal RNA production PMID: 14559904
    12. the function of Gu(alpha)in rRNA processing is at least partially dependent on its ability to interact with ribosomal protein L4. PMID: 16045751
    13. in addition to its transcriptional effects, c-Jun regulates rRNA processing and nucleolar compartmentalization of the rRNA processing protein DDX21 PMID: 18180292

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  • 亚细胞定位：
    Nucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm, cytosol. Mitochondrion.
  • 蛋白家族：
    DEAD box helicase family, DDX21/DDX50 subfamily
  • 数据库链接：

    HGNC: 2744

    OMIM: 606357

    KEGG: hsa:9188

    STRING: 9606.ENSP00000346120

    UniGene: Hs.223141