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DGKZ Antibody

  • 货号：
    CSB-PA049170
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA049170(DGKZ Antibody) at dilution 1/60, on the right is treated with synthetic peptide. (Original magnification: ×200)
    • The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA049170(DGKZ Antibody) at dilution 1/60, on the right is treated with synthetic peptide. (Original magnification: ×200)
  • 其他：

产品详情

  • Uniprot No.：
    Q13574
  • 基因名：
  • 别名：
    DAG kinase zeta antibody; DAGK5 antibody; DAGK5 PEN antibody; DAGK6 antibody; DGK ZETA antibody; DGK-zeta antibody; Dgkz antibody; DGKZ_HUMAN antibody; Diacylglycerol kinase zeta antibody; Diglyceride kinase zeta antibody; hDGKzeta antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthetic peptide of Human DGKZ
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:1000-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids. Does not exhibit an acyl chain-dependent substrate specificity among diacylglycerol species. Can also phosphorylate 1-alkyl-2-acylglycerol in vitro but less efficiently and with a preference for alkylacylglycerols containing an arachidonoyl group. The biological processes it is involved in include T cell activation since it negatively regulates T-cell receptor signaling which is in part mediated by diacylglycerol. By generating phosphatidic acid, stimulates PIP5KIA activity which regulates actin polymerization. Through the same mechanism could also positively regulate insulin-induced translocation of SLC2A4 to the cell membrane.; Regulates RASGRP1 activity.; Does not regulate RASGRP1 activity.
  • 基因功能参考文献：
    1. Results show that DGKzeta is downregulated in bone marrow mononuclear cells and associated with the severity of aplastic anemia (AA). Also, DGKzeta is a downstream target gene of miR34a. Their dysregulation enhances T-cell activation in AA cells. PMID: 28008152
    2. These results established the positive correlation between DGKzeta expression and gliomagrade. PMID: 26452358
    3. these data suggest that the activation of DGKzeta downstream of antigen recognition provides a mechanism that ensures the activation of PA-dependent signaling as a direct result of the strength of TCR-dependent DAG mobilization. PMID: 27999176
    4. Diacylglycerol kinases alpha and zeta are up-regulated in cancer in cancer, and contribute towards tumor immune evasion and T cells clonal anergy. (Review) PMID: 27697466
    5. DGKzeta knockdown engenders enhancement of NF-kappaB pathway in response to TNF-alpha. [review] PMID: 26521214
    6. Redundant and specialized roles for diacylglycerol kinases alpha and zeta in the control of T cell functions. PMID: 25921290
    7. This study shows that DGKzeta knockdown facilitates degradation of IkappaB, followed by nuclear translocation of NF-kappaB p65 subunit. PMID: 25450975
    8. Elevated DGKzeta expression contributes to increased Rho GTPase activation and the enhanced motility of metastatic cancer cells. PMID: 24646293
    9. Data indicate that after P2Y6 receptor stimulation both phospholipase D (PLD) and DGKzeta enzymes are responsible for producing phosphatidic acid (PA). PMID: 23723068
    10. DGK-zeta translocated rapidly to the plasma membrane at early stages of immunological synapse (IS) formation independent of enzyme activity; study highlights a DGKzeta-specific function for local diacylglycerol metabolism at the IS and offers new clues to its mode of regulation PMID: 21937721
    11. Antigen-specific CD8-positive T cells from DGKzeta-deficient transgenic mice show enhanced expansion and increased cytokine production after lymphocytic choriomeningitis virus infection, yet DGK-deficient memory CD8+ T cells exhibit impaired expansion. PMID: 22271650
    12. Nucleosome assembly protein (NAP) 1-like 1 (NAP1L1) and NAP1-like 4 (NAP1L4) are identified as novel DGKzeta binding partners. PMID: 21996351
    13. Data show that Diacylglycerol that 2-arachidonoyl glycerol is a very poor substrate for either the epsilon or the zeta isoforms of diacylglycerol kinases. PMID: 21194521
    14. structural domain requirements for translocation and activity PMID: 12015310
    15. PKC alpha phosphorylates diacylglycerol kinase zeta in cells, and this phosphorylation inhibits its kinase activity to remove cellular diacylglycerol, thereby affecting cell growth. PMID: 12890670
    16. role in controlling the induction of luteinizing hormone beta transcription by ERK1/2 PMID: 14707140
    17. DGKzeta generating PA, stimulates PIP5KIalpha activity to increase local PIP2, which regulates actin polymerization. PMID: 15157668
    18. DGKzeta-derived phosphatidic acid acts as a mediator of mTOR signaling PMID: 15632115
    19. DGKzeta may act in vivo as a downstream effector of pRB to regulate nuclear levels of diacylglycerol and phosphatidic acid PMID: 16286473
    20. 2,3-dioleoylglycerol binds to a site on the alpha and zeta isoforms of diacylglycerol kinase that is exposed as a consequence of the substrate binding to the active site. PMID: 18004883
    21. PKD activation is induced by DGKzeta, suggesting DGK is an upstream regulator of oxidative stress-induced activation of the PKD signaling pathway in intestinal epithelial cells. PMID: 18694729
    22. In DGKzeta-deficient fibroblasts PAK1 phosphorylation and Rac1-RhoGDI dissociation were attenuated, leading to reduced Rac1 activation after platelet-derived growth factor stimulation. PMID: 19211846
    23. None of SNPs of diacylglycerol kinase zeta tested showed association with bipolar disorder in Sardinian sample. PMID: 19308020
    24. Negative regulation of T cell receptor induced activation of the Ras-Erk1/2-AP1 pathway by DGKz PMID: 12070163

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  • 亚细胞定位：
    Nucleus. Cytoplasm, cytosol. Cell membrane. Cell projection, lamellipodium.
  • 蛋白家族：
    Eukaryotic diacylglycerol kinase family
  • 组织特异性：
    Highest levels in brain, and substantial levels in skeletal muscle, heart, and pancreas.; [Isoform 2]: Predominantly expressed in muscle.
  • 数据库链接：

    HGNC: 2857

    OMIM: 601441

    KEGG: hsa:8525

    STRING: 9606.ENSP00000412178

    UniGene: Hs.502461