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DMGDH Antibody

  • 货号:
    CSB-PA883415LA01HU
  • 规格:
    ¥440
  • 促销:
    小规格抗体限时一口价
  • 图片:
    • Western Blot
      Positive WB detected in: Mouse liver tissue, Mouse kidney tissue
      All lanes: DMGDH antibody at 6.7μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 97, 46 kDa
      Observed band size: 97 kDa
    • Immunofluorescence staining of HepG2 cells with CSB-PA883415LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • IHC image of CSB-PA883415LA01HU diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA883415LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他:

产品详情

  • 产品名称:
    Rabbit anti-Homo sapiens (Human) DMGDH Polyclonal antibody
  • Uniprot No.:
    Q9UI17
  • 基因名:
    DMGDH
  • 别名:
    Dimethylglycine dehydrogenase antibody; Dimethylglycine dehydrogenase; mitochondrial antibody; Dmgdh antibody; M2GD_HUMAN antibody; ME2GLYDH antibody; mitochondrial antibody
  • 宿主:
    Rabbit
  • 反应种属:
    Human, Mouse
  • 免疫原:
    Recombinant Human Dimethylglycine dehydrogenase, mitochondrial protein (429-524AA)
  • 免疫原种属:
    Homo sapiens (Human)
  • 标记方式:
    Non-conjugated

    本页面中的产品,DMGDH Antibody (CSB-PA883415LA01HU),的标记方式是Non-conjugated。对于DMGDH Antibody,我们还提供其他标记。见下表:

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA883415LB01HU DMGDH Antibody, HRP conjugated ELISA
    FITC CSB-PA883415LC01HU DMGDH Antibody, FITC conjugated
    Biotin CSB-PA883415LD01HU DMGDH Antibody, Biotin conjugated ELISA
  • 克隆类型:
    Polyclonal
  • 抗体亚型:
    IgG
  • 纯化方式:
    >95%, Protein G purified
  • 浓度:
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式:
    Liquid
  • 应用范围:
    ELISA, WB, IHC, IF
  • 推荐稀释比:
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols:
  • 储存条件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能:
    Catalyzes the demethylation of N,N-dimethylglycine to sarcosine. Also has activity with sarcosine in vitro.
  • 基因功能参考文献:
    1. In nonalcoholic fatty liver disease patient, the missense variant p.Ser646Pro (rs1805074) in DMGDH gene was significantly associated with disease severity and circulating levels of dimethylglycine. PMID: 27614103
    2. The structure-based analysis provided new insights into the kinetic properties of dimethylglycine dehydrogenase in particular with respect to oxygen reactivity. PMID: 27486859
    3. In agreement with previous studies, we show that the genetic variant rs921943 in DMGDH is significantly associated with selenium status in United Kingdom pregnant women. PMID: 26675765
    4. Genetic variation of DMGDH was associated with higher plasma insulin, increased insulin resistance and increased risk of incident diabetes. PMID: 25795213
    5. analysis of dimethylglycine dehydrogenase deficiency associated with pathogenic variant H109R PMID: 18937046

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  • 相关疾病:
    DMGDH deficiency (DMGDHD)
  • 亚细胞定位:
    Mitochondrion.
  • 蛋白家族:
    GcvT family
  • 数据库链接:

    HGNC: 24475

    OMIM: 605849

    KEGG: hsa:29958

    STRING: 9606.ENSP00000255189

    UniGene: Hs.655653