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DNMT3L Antibody

  • 货号：
    CSB-PA189054
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA189054(DNMT3L Antibody) at dilution 1/10, on the right is treated with fusion protein. (Original magnification: ×200)
    • The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA189054(DNMT3L Antibody) at dilution 1/10, on the right is treated with fusion protein. (Original magnification: ×200)
  • 其他：

产品详情

  • Uniprot No.：
    Q9UJW3
  • 基因名：
    DNMT3L
  • 别名：
    Cytosine 5 methyltransferase 3 like protein antibody; DNA (cytosine 5 ) methyltransferase 3 like antibody; DNA (cytosine-5)-methyltransferase 3-like antibody; DNA cytosine 5 methyltransferase 3 like protein antibody; DNA methyltransferase 3 like protein antibody; DNM3L_HUMAN antibody; Dnmt 3L antibody; Dnmt3l antibody; Human cytosine 5 methyltransferase 3 like protein antibody; MGC1090 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Fusion protein of Human DNMT3L
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:1000-1:2000
    IHC 1:10-1:50
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context. Essential for the function of DNMT3A and DNMT3B: activates DNMT3A and DNMT3B by binding to their catalytic domain. Acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3. Plays a key role in embryonic stem cells and germ cells. In germ cells, required for the methylation of imprinted loci together with DNMT3A. In male germ cells, specifically required to methylate retrotransposons, preventing their mobilization. Plays a key role in embryonic stem cells (ESCs) by acting both as an positive and negative regulator of DNA methylation. While it promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B, it also acts as an inhibitor of DNA methylation at the promoter of bivalent genes. Interacts with the EZH2 component of the PRC2/EED-EZH2 complex, preventing interaction of DNMT3A and DNMT3B with the PRC2/EED-EZH2 complex, leading to maintain low methylation levels at the promoters of bivalent genes. Promotes differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression.
  • 基因功能参考文献：
    1. DNMT3L overexpression is associated with Down syndrome. PMID: 26911678
    2. the present study has demonstrated that variations in the DNMT3L gene do not contribute to stage I-II endometriosis-associated infertility. PMID: 26647998
    3. DNMT3L rs2070565 (genotype P = 0.007, allele P = 0.0026) confers an increased risk of developing schizophrenia at an early age in individuals with family history. PMID: 24859147
    4. crystal structures of DNMT3A-DNMT3L (autoinhibitory form) and DNMT3A-DNMT3L-H3 (active form) complexes at 3.82 and 2.90 A resolution, respectively PMID: 25383530
    5. DNMT3L can address DNMT3A/B to specific sites by directly interacting with TFs that do not directly interact with DNMT3A/B PMID: 24952347
    6. CpG island encompassing the promoter and first exon of human DNMT3L gene is a PcG/TrX response element PMID: 24743422
    7. DNMT3L is one of the key players in de novo DNA methylation of imprinting control elements and retrotransposons, which occurs after genome-wide epigenetic erasure during germ cell development. (Review) PMID: 22671959
    8. SNP rs2070565, as well as haplotypes AAA and GAA, may be associated with male infertility; DNMT3L may contribute to azoospermia susceptibility in humans PMID: 22116073
    9. Genetic polymorphisms of DNMT3L involved in hypermethylation of chromosomal ends are associated with greater risk of developing ovarian endometriosis. PMID: 22401780
    10. mutation analysis of SYCP3, DNMT3L and MSH4 in patients with maturation arrest of spermatogenesis and couples with recurrent miscarriages. PMID: 21126912
    11. This study offers insights into the manner by which DNA methylation patterns are deposited and reveals a new level of interplay between members of the de novo DNMT family. PMID: 20838592
    12. Processive methylation is enhanced 3-fold in the presence of DNMT3L, an inactive homolog of DNMT3A, therefore providing a mechanism for the previously described DNMT3L activation of DNMT3A. PMID: 20630873
    13. We have identified loss of methylation at the DNMT3L promoter in ocular surface squamous neoplasia cases. PMID: 20670142
    14. DNMT3L is a novel marker and is essential for the growth of human embryonal carcinoma. PMID: 20460473
    15. DNMT3L exerts a major effect on the transcriptional regulation of a specific target gene, such as thymine DNA glycosylase PMID: 20428781
    16. CG dinucleotide recognized by the Dnmt3a and Dnmt3L complex are distinctive at retroelements and imprinted domains. PMID: 19921333
    17. DNMT3L stimulates de novo methylation by Dnmt3a. PMID: 12481029
    18. The carboxyl-terminal half of DNMT3L was found to be responsible for the stimulation of the DNA methylation activity of Dnmt3a and Dnmt3b. PMID: 15105426
    19. Regulates germ cell-specific gene expression and intracisternal A-particle suppression, which are critical for male germ cell proliferation and meiosis. PMID: 16211598
    20. Dnmt3L-Dnmt3b interactions play an important role in the regulation of DNA methylation during mammalian development. PMID: 16543361
    21. The acquisition of DNMT3L by a common ancestor of eutherians and marsupials might have been closely related to the evolution of imprinting. PMID: 16575165
    22. Data show that binding of DNMT3L to DNMT3A2 promotes reorganization of DNMT3A2 subunits and leads to formation of specific complexes with enhanced DNA methyltransferase activity and increased S-adenosyl-L-methionine binding. PMID: 16829525
    23. Crystallographic studies of human DNMT3L showed that the protein has a carboxy-terminal methyltransferase-like domain and an N-terminal cysteine-rich domain PMID: 17687327
    24. the carboxy-terminal domain of human Dnmt3L interacts with the catalytic domain of Dnmt3a, demonstrating that Dnmt3L has dual functions of binding the unmethylated histone tail and activating DNA methyltransferase PMID: 17713477
    25. Importance of DNA methylation profile at DNMT3L promoter not only as a promising biomarker for cervical cancer but also provides insight into the possible role of DNMT3L in cancer development. PMID: 17965599
    26. DNMT3L(R271Q) is impaired in its ability to stimulate de novo DNA methylation by DNMT3A. PMID: 19246518
    27. Overexpression of DNMT3L, which functions by regulating the activity of DNMT3A and DNMT3B, increased cellular proliferation and anchorage-independent growth. PMID: 19625766

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Expressed at low levels in several tissues including testis, ovary, and thymus.
  • 数据库链接：

    HGNC: 2980

    OMIM: 606588

    KEGG: hsa:29947

    STRING: 9606.ENSP00000270172

    UniGene: Hs.592165