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DUT Antibody

  • 货号：
    CSB-PA926314
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA926314(DUT Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)
    • Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane: 293T cells, Primary antibody: CSB-PA926314(DUT Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes
  • 其他：

产品详情

  • Uniprot No.：
    P33316
  • 基因名：
    DUT
  • 别名：
    Deoxyuridine 5' triphosphate nucleotidohydrolase; nuclear isoform antibody; Deoxyuridine 5'-triphosphate nucleotidohydrolase; mitochondrial antibody; Deoxyuridine triphosphatase antibody; dut antibody; DUT_HUMAN antibody; dUTP nucleotidohydrolase antibody; dUTP pyrophosphatase antibody; dUTPase antibody; FLJ20622 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Rat
  • 免疫原：
    Synthetic peptide of Human DUT
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:1000-1:2000
    WB 1:200-1:1000
    IHC 1:10-1:50
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
  • 基因功能参考文献：
    1. A single homozygous missense mutation (chr15.hg19:g.48,626,619A>G) located in the dUTPase (DUT) gene was associated with a syndrome of bone marrow failure and diabetes. PMID: 28073829
    2. Comparison of the cellular distribution of wild-type dUTPase with those of hyperphosphorylation- and hypophosphorylation-mimicking mutants suggests that phosphorylation at Ser11 leads to the exclusion of dUTPase from the nucleus. PMID: 24311590
    3. EBV-encoded dUTPase may act as an intercellular signaling molecule capable of modulating the cellular microenvironment PMID: 23894549
    4. Data sugget that inhibition of dUTPase is a mechanism-based therapeutic approach to significantly enhance the efficacy of thymidylate synthase (TS)-targeted chemotherapeutic agents. PMID: 22172489
    5. Repression of dUTPase induced specific expression level increments for thymidylate kinase and thymidine kinase, and also an increased sensitization to 5-fluoro-2'-deoxyuridine and 5-fluoro-uracil. PMID: 21780905
    6. High dUTP pyrophosphatase is associated with recurrence in colorectal cancer patients undergoing 5-fluorouracil adjuvant chemotherapy PMID: 20560137
    7. nuclear dUTPase may be a good biomarker for predicting prognosis in HCC patients after surgical resection PMID: 19968781
    8. significant differences between the human and Plasmodium dUTPases PMID: 19879316
    9. Novel gain of function activity of p53 mutants: activation of the dUTPase gene expression leading to resistance to 5-fluorouracil. PMID: 12096336
    10. Nuclear dUTPase may contain a complex nuclear localization signal that is located throughout the entire protein. PMID: 12799180
    11. dUTPase staining provides a useful measure of cell proliferation distinct from that offered by Ki-67 labeling. It proved particularly useful for the evaluation of diffuse astrocytomas. PMID: 16325515
    12. kinetic model of the human dUTPase catalytic cycle PMID: 17848562
    13. determined the crystal structure of the enzyme:alpha,beta-imino-dUTP PMID: 17880943
    14. Establishment of a direct role for both mutant and wild-type forms of p53 in modulating dUTPase promoter activity. PMID: 19015155
    15. hinge proline destabilize human and Escherichia coli dUTPases without preventing trimeric organization PMID: 19302784

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  • 亚细胞定位：
    [Isoform 2]: Nucleus.; [Isoform 3]: Mitochondrion.
  • 蛋白家族：
    DUTPase family
  • 组织特异性：
    Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein leve
  • 数据库链接：

    HGNC: 3078

    OMIM: 601266

    KEGG: hsa:1854

    STRING: 9606.ENSP00000370376

    UniGene: Hs.527980