Your Good Partner in Biology Research

  1. Home
  2. 抗体
  3. 多克隆抗体
  4. ECT2 Antibody

ECT2 Antibody

  • 货号：
    CSB-PA888001LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA888001LA01HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunoprecipitating ECT2 in Hela whole cell lysate
      Lane 1: Rabbit control IgG instead of CSB-PA888001LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000)
      Lane 2: CSB-PA888001LA01HU (6μg) + Hela whole cell lysate (1mg)
      Lane 3: Hela whole cell lysate (20μg)
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, 293T whole cell lysate
      All lanes:ECT2 antibody at 2.7μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 104, 100, 109, 101 kDa
      Observed band size: 104 kDa
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) ECT2 Polyclonal antibody
  • Uniprot No.：
    Q9H8V3
  • 基因名：
    ECT2
  • 别名：
    ECT2Protein ECT2 antibody; Epithelial cell-transforming sequence 2 oncogene antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Protein ECT2 protein (283-453AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，ECT2 Antibody (CSB-PA888001LA01HU)，的标记方式是Non-conjugated。对于ECT2 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA888001LB01HU ECT2 Antibody, HRP conjugated ELISA
    FITC CSB-PA888001LC01HU ECT2 Antibody, FITC conjugated
    Biotin CSB-PA888001LD01HU ECT2 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:500-1:1000
    IP 1:200-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.
  • 基因功能参考文献：
    1. High ECT2 expression was correlated with tumor metastasis and poor overall survival of osteosarcoma patients. PMID: 28794404
    2. The expression of ECT2 is related to the survival of patients with breast cancer, and Its high expression is significantly associated with unfavourable survival rates. PMID: 29051317
    3. These results identified p53 as a novel up-stream signaling molecule of ECT2 in Gastric cancer cells. PMID: 28654632
    4. High PDC2 expression is associated with pancreatic adenocarcinoma. PMID: 26993610
    5. While the association of ECT2 with the plasma membrane is essential for cytokinesis, our data suggest that ECT2 recruitment to the spindle midzone is insufficient to account for equatorial furrowing and may act redundantly with yet-uncharacterized signals. PMID: 27926870
    6. Among 518 genes co-expressed with ECT2 in LUAD and 386 genes co-expressed with ECT2 in LUSC, there were only 98 genes in the overlapping cluster PMID: 29088286
    7. E6AP suppresses breast cancer metastasis by regulating actin cytoskeleton remodeling through the control of ECT2 and Rho GTPase activity. PMID: 27231202
    8. Ect2 regulates rRNA synthesis through a PKCiota-Ect2-Rac1-NPM signaling axis that is required for lung tumorigenesis. PMID: 28110998
    9. Data suggest that the expression of epithelial cell transforming sequence 2 oncogene (ECT2) can serve as an alternative measurement that can compensate for the inadequacy of the current carcinoembryonic antigen (CEA) test in the diagnosis and monitoring of colorectal cancer patients. PMID: 28362321
    10. Our data demonstrate that the cytokinetic proteins epithelial cell transforming 2 and Aurora kinase B (AurkB) are localized to stress granules in human astrocytoma cells PMID: 27106762
    11. Kaplan-Meier analysis revealed that lower levels of Ect2 mRNA predicted higher overall survivals and biochemical recurrence (BCR)-free survivals in all patients or non-metastatic patients. PMID: 28012134
    12. Our results suggest that ECT2 plays an important role during gastric cancer progression PMID: 26497353
    13. Colorectal cancer patients with high expression levels of ECT2 had shorter overall survival. PMID: 26211594
    14. ECT2 can crosstalk with RACGAP1 to catalyse the GTP exchange involved in Rho signalling to further regulate tumour initiation and metastasis. PMID: 25617497
    15. Up-regulation of ECT2 might contribute to the progression of gastric carcinogenesis and may be a useful prognostic indicator in gastric cancer. PMID: 25674238
    16. Poly(ADP-ribosyl)ation is recognized by ECT2. PMID: 25486481
    17. central spindle assembly and 2 Plk1-dependent phosphorylations are required to establish efficient binding of the Ect2 BRCT in early cytokinesis. PMID: 25486482
    18. ECT2 expression is positively correlated with WHO pathologic grading and unfavorable survival, suggesting that ECT2 may be a potential therapeutic candidate in human gliomas. PMID: 25237947
    19. structure of triple-BRCT-domain of ECT2 and insights into the binding characteristics to CYK-4 PMID: 25068414
    20. the deregulation of miR-223 and its target gene ECT2 may be associated with the aggressive tumor progression of human osteosarcoma PMID: 24784921
    21. Abnormality of the ECT2 gene occurs at a relatively early stage of lung adenocarcinogenesis and would be applicable as a new biomarker for prognostication of patients with lung adenocarcinoma PMID: 24484057
    22. Both Pbl and ECT2 repress Wg/Wnt target gene expression in cultured Drosophila and human cells. PMID: 24198276
    23. Data suggest that ECT2 may play an oncogenic role in the pancreatic ductal adenocarcinoma (PDAC) neoplastic process. PMID: 23851435
    24. miR-223 functions as a tumor suppresser in osteosarcoma and miR-223/Ect2/p21 signaling is an important pathway that regulates the osteosarcoma cell cycle progression and proliferaion PMID: 23601845
    25. ECT2 is important for tight junction function and maintenance of cell polarity. Nonfunction of this gene may cause renal tubulointerstitial injury, progressing to glomerular sclerosis. PMID: 22552385
    26. RASAL2 was identified as an ECT2-interacting protein that regulates RHO activity in astrocytoma cells. PMID: 22683310
    27. find that Ect2 first becomes active in prophase, when it is exported from the nucleus into the cytoplasm, activating RhoA to induce the formation of a mechanically stiff and rounded metaphase cortex PMID: 22898780
    28. Data supports an analogous function for the anillin-Ect2 complex in human cells and one hypothesis is that this complex has functionally replaced the Drosophila anillin-RacGAP50C complex. PMID: 22514687
    29. identify Ect2 as a cell cycle-regulated protein and suggest that its ubiquitination-dependent degradation may play an important role in RhoA regulation at the time of mitosis. PMID: 21886810
    30. targeting of Ect2 to the equatorial membrane represents a key step in the delivery of the cytokinetic signal to the cortex PMID: 22172673
    31. A mechanism involving the nuclear GEFs Ect2 and Net1 for activating RhoB after genotoxic stress, thereby facilitating cell death after treatment with DNA damaging agents. PMID: 21373644
    32. Ect2 was a possible regulator of matrix-contact-side localization of invadopodia-related proteins. PMID: 21474972
    33. a model in which PKCiota-mediated phosphorylation regulates Ect2 binding to the oncogenic PKCiota-Par6 complex thereby activating Rac1 activity and driving transformed growth and invasion. PMID: 21189248
    34. Results suggest that ECT2 is an indicator of cellular proliferation in OSCCs and that ECT2 might be a potential therapeutic target for the development of new treatments for OSCCs. PMID: 21124766
    35. XRCC1, CLB6, and BRCT domains of ECT2 play a critical role in regulating cytokinesis PMID: 14587037
    36. ECT2 regulates the polarity complex Par6/Par3/PKCzeta and possibly plays a role in epithelial cell polarity PMID: 15254234
    37. BRCT domains negatively regulate Ect2 GEF activity in interphase cells, and they are also required for the proper function of Ect2 during cytokinesis PMID: 15545273
    38. Ect2 regulates the activation and function of Cdc42 in mitosis. PMID: 15642749
    39. Central spindle localization of ECT2 assists division plane positioning and the CYK-4 subunit of centralspindlin acts upstream of RhoA to promote furrow assembly. PMID: 16103226
    40. Cdk1 inactivation is sufficient to activate a signaling pathway leading to cytokinesis, which emanates from mitotic spindles and is regulated by ECT2, MgcRacGAP, and RhoA PMID: 16118207
    41. MgcRacGAP controls the initiation of cytokinesis by regulating ECT2, which in turn induces the assembly of the contractile ring and triggers the ingression of the cleavage furrow PMID: 16129829
    42. a conformational change of ECT2 upon phosphorylation at T341. Therefore, ECT2 activity might be regulated by the phosphorylation status of T341. PMID: 16170345
    43. ECT2 is regulated by Plk1 and CDK1, phosphorylation of ECT2 leads to accumulation of RHOA PMID: 16247472
    44. Data show that RhoA accumulates at the equatorial cortex before furrow initiation and continues to concentrate at the cleavage furrow during cytokinesis, and that centralspindlin and ECT2 are required for this localization and furrowing. PMID: 16352658
    45. ECT2 knockdown triggers cell cycle arrest in G1. PMID: 16778203
    46. In mitotic cells Ect2 localizes to the central spindle and to the cell cortex. PMID: 16803869
    47. Aberrant ECT2 expression, observed in various human tumors, could be the direct result of RB/E2F pathway deficiency, thereby contributing to cell division in cancers. PMID: 16862181
    48. Late mitotic Plk1 activity promotes recruitment of Ect2 to the central spindle, triggering the initiation of cytokinesis and contributing to cleavage plane specification in human cells. PMID: 17488623
    49. This gene was silenced. PMID: 17688947
    50. These results suggest that equatorial Ect2 locally suppresses lamellipodia formation via RhoA activation, which indirectly contributes to restricting lamellipodia formation to polar regions during cytokinesis B. PMID: 17942602

    显示更多

    收起更多

  • 亚细胞定位：
    Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Cleavage furrow. Midbody. Cell junction. Cell junction, tight junction. Note=Sequestered within the nucleus during interphase. Dispersed throughout the cytoplasm upon breakdown of the nuclear envelope during mitosis. Colocalizes with the centralspindlin complex to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalized with RhoA at the midbody. Its subcellular localization to tight junction is increased by calcium. Localized predominantly in the cytoplasm of numerous carcinoma cells.
  • 组织特异性：
    Expressed in lung epithelial cells (at protein level). Expressed in squamous cell carcinoma, primary non-small cell lung cancer tumors and lung adenocarcinoma.
  • 数据库链接：

    HGNC: 3155

    OMIM: 600586

    KEGG: hsa:1894

    STRING: 9606.ENSP00000232458

    UniGene: Hs.518299