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EFNB1 Antibody

  • 货号：
    CSB-PA002376
  • 规格：
    ￥880
  • 图片：
    • Western Blot analysis of COS7 cells using Ephrin-B1 Polyclonal Antibody
  • 其他：

产品详情

  • Uniprot No.：
    P98172
  • 基因名：
    EFNB1
  • 别名：
    EFNB1; EFL3; EPLG2; LERK2; Ephrin-B1; EFL-3; ELK ligand; ELK-L; EPH-related receptor tyrosine kinase ligand 2; LERK-2
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat,Monkey
  • 免疫原：
    Synthesized peptide derived from Human Ephrin-B1 around the non-phosphorylation site of Y317.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    ELISA 1:40000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binding to Eph receptors residing on adjacent cells leads to contact-dependent bidirectional signaling into neighboring cells. Shows high affinity for the receptor tyrosine kinase EPHB1/ELK. Can also bind EPHB2 and EPHB3. Binds to, and induces collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons.
  • 基因功能参考文献：
    1. Lymphomas with low UTX expression express high levels of Efnb1, and cause significantly poor survival. PMID: 30006524
    2. Chronic hypoxia-induced slug promotes invasive behavior of prostate cancer cells by activating the expression of ephrin-B1. PMID: 30058095
    3. This study showed that EphB2 cells have a transient increase in migration after heterotypic activation, which underlies a shift in the EphB2-ephrinB1 border but is not required for segregation or border sharpening. PMID: 28747399
    4. that expression of EFNB1 and EFNB2 is implicated in Th cell differentiation and migration to inflammatory sites in both EAE and MS PMID: 27039370
    5. we demonstrate that mosaicism for EPHRIN-B1 expression induced by random X inactivation in heterozygous females results in robust cell segregation in human neuroepithelial cells, thus supplying experimental evidence that Eph/ephrin-mediated cell segregation is relevant to pathogenesis in human CFNS patients. PMID: 28238796
    6. one novel (IVS2+3G>T) and one previously reported mutation (p.Gly151Ser) in EFNB1 Both patients were de novo cases without a family history of Craniofrontonasal syndrome. PMID: 27650623
    7. While ephrin-B1 deficiency leads to abnormal visual pathways in mice, it leaves the human visual system, apart from deficits in binocular vision, largely normal. PMID: 26580852
    8. that EphrinB1 (EFNB1) co-localizes with microtubules (MTs) during all phases of the cell cycle. PMID: 25436983
    9. we report a family with a G151S mutation in the EFNB1 gene. The mutation was identified in two severely affected sisters and paradoxically in their clinically unaffected father. PMID: 25486017
    10. T cells from rheumatoid arthritis (RA) patients expressed higher EFNB1 mRNA levels, which correlated with RA symptoms and laboratory findings. Expression of EFNB1 in T cells might be a parameter for monitoring RA disease activity and treatment responses. PMID: 25779027
    11. Results indicate that EphrinB1 is uniquely dysregulated in medulloblastoma and promotes oncogenic responses in medulloblastoma cells, implicating ephrinB1 as a potential target PMID: 25258252
    12. EphB2/ephrin-B1 were invoked in dental pulp stem cells with TNF-alpha treatment via the JNK-dependent pathway, but not NF-kB, p38 MAPK or MEK signalling. PMID: 25643922
    13. Patients with EFNB1 mutations have a clear phenotype. This study will facilitate genetic counseling of parents and patients, and contribute to the diagnostic and screening process of patients with suspected CFNS. PMID: 24281372
    14. CNK1 mediates ephrinB1 signaling that promotes cell migration through RhoA and JNK activity. PMID: 24825906
    15. EphrinB1 expression is related to the metastasis of breast cancer and its enhanced expression confers a poor prognosis, suggesting that EphrinB1 may be a relevant therapeutic target in breast cancers. PMID: 24240587
    16. High EFNB1 expression is associated with squamous cell carcinomas of the head and neck. PMID: 23811940
    17. we report the identification of mosaic EFNB1 mutations in every individual with craniofrontonasal syndrome, confirming the suggested diagnosis and supporting the hypothesis of cellular interference in humans. PMID: 23335590
    18. These results indicate a novel function of Nm23-H1 to control contact inhibition of locomotion, and its negative regulation by ephrin-B1. PMID: 22718351
    19. Data show that EphrinB1, a PTPN13 substrate, interacts with ErbB2, and Src kinase mediates EphrinB1 phosphorylation and subsequent MAP Kinase signaling. PMID: 22279592
    20. X-linked cases resembling Teebi hypertelorism may have a similar mechanism to CFNS, and that cellular mosaicism for different levels of ephrin-B1 (as well as simple presence/absence) leads to craniofacial abnormalities. PMID: 21542058
    21. Unreported mutation in EFNB1 predicts occurrence of congenital diaphragmatic hernia, phenotypic differences between males and female. PMID: 20734337
    22. The impact of craniofrontonasal syndrome-causing EFNB1 mutations on ephrin-B1 function is reported. PMID: 20565770
    23. study reports that the expression of ephrin-B1 is controlled by a feedback loop involving posttranscriptional regulatory mechanisms PMID: 20308325
    24. combination of SDF-1, PTN, IGF2, and EFNB1 mimics the DA phenotype-inducing property of SDIA and was sufficient to promote differentiation of hESC to functional midbrain DA neurons PMID: 19672298
    25. Human platelets express EphA4 and EphB1, and the ligand, ephrinB1. Forced clustering of EphA4 or ephrinB1 led to cytoskeletal reorganization, adhesion to fibrinogen, and alpha-granule secretion. PMID: 12084815
    26. Expression profile of this ligand of EPHB2 in gastric cancer PMID: 12136247
    27. The expression of ephrin-B1 was correlated with a poorer clinical prognosis. Ephrin-B1 protein was expressed by osteosarcoma cells and blood vessels. PMID: 12209731
    28. Results identified a novel interaction between EphB1 with the adaptor molecule Grb7 and suggested that this interaction may play a role in the regulation of cell migration by EphB1. PMID: 12223469
    29. expression of Eph-B1 in plasmacytoid dendritic cells PMID: 12384430
    30. Ephrin B1 induces human aortic endothelial cells migration in a pathway that involves Crk adaptor protein PMID: 12475948
    31. conclude that ephrinB1 and B class Eph receptors provide positional cues required for the normal morphogenesis of skeletal elements PMID: 12919674
    32. Eph/ephrin signaling enhances the ability of platelet agonists to cause aggregation by activating Rap1 and these effects require oligomerization of ephrinB1 but not phosphotyrosine-based interactions with the ephrinB1 cytoplasmic domain. PMID: 14576067
    33. Ephrin-B1 may be involved in in vivo tumor progression by promoting neovascularization in hepatocellular carcinoma PMID: 14642617
    34. Mutations of the ephrin-B1 gene cause craniofrontonasal syndrome PMID: 15124102
    35. ephrin-B1 internalization is an active receptor-mediated process that utilizes the clathrin-mediated endocytic pathway PMID: 15351694
    36. On ephrinB1 stimulation, the small GTPases Rho and Ras are activated and Rap1 is inactivated. PMID: 15725075
    37. EFNB1 mutations have a role in familial and sporadic craniofrontonasal syndrome (CFNS) PMID: 15959873
    38. EphB/ephrin-B molecules play a role in restricting dental pulp stem cell attachment and migration to maintain Dthesse cells within their stem cell niche under steady-state conditions. PMID: 17204606
    39. C-terminus of ephrin-B1 regulates activation of the extracellular release of MMP-8 without requirement of de novo protein synthesis. PMID: 17567680
    40. Our results suggest that tyrosine phosphorylation of ephrin-B1 promotes invasion of cancer cells in vivo PMID: 17591954
    41. Silencing of EphB expression is associated with colorectal tumorigenesis. PMID: 17906625
    42. EphrinB1 may play an important role in the inflammatory states of rheumatoid arthritis, by affecting the population/function of T cells. PMID: 17942634
    43. EFNB1 mutant transcripts with nonsense codons are degraded by the nonsense-mediated mRNA decay pathway and lead to craniofrontonasal syndrome. PMID: 18043713
    44. Ephrin-B1 is likely to play an important role in the regulation of malignant T lymphocytes through the control of lipid-raft-associated signaling, adhesion, and invasive activity PMID: 18314490
    45. Several new EFNB1 mutations have been identified in craniofrontonasal syndrome. PMID: 18627045

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  • 相关疾病：
    Craniofrontonasal syndrome (CFNS)
  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Membrane raft.; [Ephrin-B1 C-terminal fragment]: Cell membrane; Single-pass type I membrane protein.; [Ephrin-B1 intracellular domain]: Nucleus.
  • 蛋白家族：
    Ephrin family
  • 组织特异性：
    Widely expressed. Detected in both neuronal and non-neuronal tissues. Seems to have particularly strong expression in retina, sciatic nerve, heart and spinal cord.
  • 数据库链接：

    HGNC: 3226

    OMIM: 300035

    KEGG: hsa:1947

    STRING: 9606.ENSP00000204961

    UniGene: Hs.144700