Your Good Partner in Biology Research

  1. Home
  2. 抗体
  3. 多克隆抗体
  4. EIF4G2 Antibody

EIF4G2 Antibody

  • 货号：
    CSB-PA007569LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate
      All lanes: EIF4G2 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 103, 99 kDa
      Observed band size: 99 kDa
    • IHC image of CSB-PA007569LA01HU diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA007569LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) EIF4G2 Polyclonal antibody
  • Uniprot No.：
    P78344
  • 基因名：
    EIF4G2
  • 别名：
    DAP-5 antibody; DAP5 antibody; Death associated protein 5 antibody; Death-associated protein 5 antibody; eIF-4-gamma 2 antibody; eIF-4G 2 antibody; eIF4G 2 antibody; EIF4G2 antibody; Eukaryotic translation initiation factor 4 gamma 2 antibody; IF4G2_HUMAN antibody; Nat1 antibody; Novel APOBEC-1 target 1 antibody; p97 antibody; Translation repressor NAT1 antibody; Translation repressor NAT1 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Eukaryotic translation initiation factor 4 gamma 2 protein (706-907AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，EIF4G2 Antibody (CSB-PA007569LA01HU)，的标记方式是Non-conjugated。对于EIF4G2 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA007569LB01HU EIF4G2 Antibody, HRP conjugated ELISA
    FITC CSB-PA007569LC01HU EIF4G2 Antibody, FITC conjugated
    Biotin CSB-PA007569LD01HU EIF4G2 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.
  • 基因功能参考文献：
    1. The percentage at the S-stage was decreased in the miR-379 mimics and EIF4G2 shRNA groups. PMID: 28381518
    2. DAP5 knockdown from human ESCs (hESCs) resulted in persistence of pluripotent gene expression, delayed induction of differentiation-associated genes in different cell lineages, and defective embryoid body formation PMID: 27664238
    3. Data show that microRNA miR-379 potentiated lung cancer (LCa) chemosensitivity via modulation of cisplatin (CDDP)-induced apoptosis by directly targeting the eukaryotic translation initiation factor 4 gamma 2 (EIF4G2) 3' UTR. PMID: 28117895
    4. These results indicate that miR-139 is capable of inhibiting chondrocyte proliferation and migration, thus being a possible therapeutic target for OA. The mechanism of miR-139 in chondrocytes may be related to its regulation on EIF4G2 and IGF1R. PMID: 27105918
    5. The Coxsackievirus B3 protease 2A-mediated cleavage of DAP5 results in the production of two truncates that exert differential effects on protein translation of the IRES-containing genes, leading to enhanced host cell death. PMID: 26586572
    6. Knockdown of EIF4G2 recapitulated the effects of mir-139, whereas restoring EIF4G2 expression rescued the mir-139 phenotype. elevated miR-139-5p expression is associated with a favorable outcome in acute myeloid leukemia. PMID: 26165837
    7. findings provide the first mechanistic insights into the function of DAP5 as a selective regulator of cap-independent translation PMID: 25779044
    8. Our results provide evidence that the tumor suppressor effect of miR-520c-3p is mediated through repression of translation while inducing senescence and that eIF4GII is a key effector of this anti-tumor activity. PMID: 24497838
    9. DAP5, a translation initiation factor shown to positively regulate the translation of various internal ribosome entry sites containing mRNAs, promotes internal ribosome entry site-driven translation of p53 mRNA. PMID: 23318444
    10. DAP5/p97 and DAP5/p86 enhanced the translation of the anti-apoptotic protein Bcl-2 and inhibited cisplatin-induced apoptosis PMID: 22555068
    11. Multiple isoforms of eIF4GII arise from multiple promoters & alternative splicing events. A non-canonical CUG initiation codon extends the eIF4GII N-terminus. The eIF4GII N-terminus plays an alternative role in initiation factor assembly. PMID: 22909319
    12. crystal structure of C-terminal region (residues 540-897) of p97/EIF4G2 (at 2.0 Angstrom resolution): sequence/structure homology with EIF4G1 PMID: 20544972
    13. crystallization and preliminary X-ray diffraction analysis of the MIF4G domain of DAP5 is presented. PMID: 20057060
    14. Includes the functional analysis of a similar protein in yeast. PMID: 11331597
    15. The caspase-cleaved DAP5 protein supports internal ribosome entry site-mediated translation of death proteins PMID: 11943866
    16. endogenous eIF4GII was phosphorylated by Ca(2+)/calmodulin-dependent protein kinase I PMID: 14507913
    17. Cleavage of eIF4GII is induced in cells and in cell extracts by the FMDV leader protease (L(pro)) alone PMID: 15016848
    18. EIF4G2 transcripts exhibited three different expression patterns, downregulation was found in about 50% of the cases analyzed and EIF4G2 gene transcription was associated with invasive tumors. PMID: 16410684
    19. p97 is functionally different from the closely related C-terminal two-thirds of eIF4GI and it can positively promote protein synthesis and cell proliferation. PMID: 16932749
    20. These data indicate that full-length p97 is a translational activator with essential role(s) in unstressed cells, suggesting a reassessment of current models of p97 function. PMID: 17237356
    21. Data show that DAP5 promotes cap-independent translation of Bcl-2 and CDK1 to facilitate cell survival during mitosis. PMID: 18450493
    22. role and regulation of death-associated protein-5 (DAP5/p97/NAT1), a novel inhibitor of translational initiation, in acute promyelocytic leukemia cell differentiation and apoptosis PMID: 18491231
    23. Results report the crystal structure of the C-terminal region of DAP5/p97 extending between amino acids 730 and 897. PMID: 18722383
    24. Exclusive use of a GUG translation initiation codon is conserved in human, chicken, Xenopus, and zebrafish. PMID: 15718103
    25. translation starts exclusively at a non-AUG translation initiation codon (GUG). PMID: 9049310

    显示更多

    收起更多

  • 蛋白家族：
    Eukaryotic initiation factor 4G family
  • 组织特异性：
    Ubiquitously expressed in all adult tissues examined, with high levels in skeletal muscle and heart. Also expressed in fetal brain, lung, liver and kidney.
  • 数据库链接：

    HGNC: 3297

    OMIM: 602325

    KEGG: hsa:1982

    STRING: 9606.ENSP00000340281

    UniGene: Hs.183684