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ENPP7 Antibody

  • 货号：
    CSB-PA751012LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: K562 whole cell lysate, HeLa whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Mouse heart tissue, Mouse kidney tissue
      All lanes: ENPP7 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 52 kDa
      Observed band size: 52 kDa
    • Immunofluorescence staining of HepG2 cells with CSB-PA751012LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) ENPP7 Polyclonal antibody
  • Uniprot No.：
    Q6UWV6
  • 基因名：
    ENPP7
  • 别名：
    ENPP7; UNQ3077/PRO9912; Ectonucleotide pyrophosphatase/phosphodiesterase family member 7; E-NPP 7; NPP-7; Alkaline sphingomyelin phosphodiesterase; Intestinal alkaline sphingomyelinase; Alk-SMase
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse
  • 免疫原：
    Recombinant Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 7 protein (22-216AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，ENPP7 Antibody (CSB-PA751012LA01HU)，的标记方式是Non-conjugated。对于ENPP7 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA751012LB01HU ENPP7 Antibody, HRP conjugated ELISA
    FITC CSB-PA751012LC01HU ENPP7 Antibody, FITC conjugated
    Biotin CSB-PA751012LD01HU ENPP7 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IF 1:200-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Choline-specific phosphodiesterase that hydrolyzes sphingomyelin releasing the ceramide and phosphocholine and therefore is involved in sphingomyelin digestion, ceramide formation, and fatty acid (FA) absorption in the gastrointestinal tract. Has also phospholipase C activity and can also cleave phosphocholine from palmitoyl lyso-phosphatidylcholine and platelet-activating factor (PAF) leading to its inactivation. Does not have nucleotide pyrophosphatase activity. May promote cholesterol absorption by affecting the levels of sphingomyelin derived from either diet or endogenous sources, in the intestinal lumen.
  • 基因功能参考文献：
    1. human alkaline sphingomyelinase crystal structure provides insights into substrate recognition PMID: 28292932
    2. NPP7 activity and the ratio of 1.4/1.2 kb products in bile are significantly decreased in malignancy, particularly in cholangiocarcinoma. PMID: 25100243
    3. The F275A mutation of NPP7 showed impaired catalytic function whereas L107F mutation showed enhanced catalytic activity. PMID: 22177013
    4. predict the three-dimensional structure of NPP7 by homology modeling using a recently crystallized NPP from bacteria. Using the model, we studied the substrate specificity of the enzyme by docking. PMID: 20839774
    5. identified the amino acid and cDNA sequences of human intestinal alk-SMase, and found that it is a novel ecto-enzyme related to the ecto-nucleotide phosphodiesterase family with specific features essential for its SMase activity PMID: 12885774
    6. intestinal alkaline sphingomyelinase may have a one-exon deletion in colon cancer cells PMID: 15016655
    7. alk-SMase activity is severely affected by defective N-glycosylation at 5 sites and by structural alterations of the putative metal-binding sites and the predicted active core. PMID: 15458386
    8. Results describe the cloning of rat alkaline sphingomyelinase from rat intestine, comparison to the human sequence, adjustment of the putative protein in GenBank, and confirmation of the specific expression of the gene in the small intestine. PMID: 15708357
    9. Alkaline sphingomyelinase hydrolyses and inactivates PAF by a phospholipase C activity, a novel function by which it may counteract the development of intestinal inflammation and colon cancer. PMID: 16255717

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Nucleotide pyrophosphatase/phosphodiesterase family
  • 组织特异性：
    Detected in the colon (at protein level). Expressed in the duodenum, jejunum and liver and at low levels in the ileum. Expression was very low in the esophagus, stomach and colon.
  • 数据库链接：

    HGNC: 23764

    OMIM: 616997

    KEGG: hsa:339221

    STRING: 9606.ENSP00000332656

    UniGene: Hs.114084