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FAN1 Antibody

  • 货号：
    CSB-PA22859A0Rb
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: PC-3 whole cell lysate
      All lanes: FAN1 antibody at 5.3μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 115, 60 kDa
      Observed band size: 115 kDa
    • IHC image of CSB-PA22859A0Rb diluted at 1:300 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

产品详情

  • 产品描述：

    The product CSB-PA22859A0Rb is a rabbit polyclonal antibody raised against recombinant human FAN1 (167-373aa). This FAN1 antibody is purified by protein G and gets a purity of up to 95%. It can recognize and detect human FAN1 protein in ELISA, WB, and IHC applications.

    The FAN1 protein acts as a nuclease enzyme that plays a critical role in the repair of DNA damage, especially the interstrand crosslinks (ICLs). It has also been implicated in other cellular processes, including telomere maintenance and genome stability. Dysregulation of FAN1 expression or function has been associated with various pathological conditions, including cancer, neurodegenerative diseases, and autoimmune disorders.

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) FAN1 Polyclonal antibody
  • Uniprot No.：
    Q9Y2M0
  • 基因名：
    FAN1
  • 别名：
    6030441H18Rik antibody; Coiled coil domain containing protein MTMR15 antibody; DKFZp451H236 antibody; DKFZp686K16147 antibody; FAN1 antibody; FAN1_HUMAN antibody; FANCD2 and FANCI associated nuclease 1 antibody; FANCD2/FANCI associated nuclease 1 antibody; FANCD2/FANCI-associated nuclease 1 antibody; Fanconi associated nuclease 1 antibody; Fanconi-associated nuclease 1 antibody; hFAN1 antibody; KIAA1018 antibody; KMIN antibody; MTMR 15 antibody; MTMR15 antibody; Myotubularin related protein 15 antibody; Myotubularin-related protein 15 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Fanconi-associated nuclease 1 protein (167-373AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，FAN1 Antibody (CSB-PA22859A0Rb)，的标记方式是Non-conjugated。对于FAN1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA22859B0Rb FAN1 Antibody, HRP conjugated ELISA
    FITC CSB-PA22859C0Rb FAN1 Antibody, FITC conjugated
    Biotin CSB-PA22859D0Rb FAN1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates. Not involved in DNA double-strand breaks resection. Acts as a 5'-3' exonuclease that anchors at a cut end of DNA and cleaves DNA successively at every third nucleotide, allowing to excise an ICL from one strand through flanking incisions. Probably keeps excising with 3'-flap annealing until it reaches and unhooks the ICL. Acts at sites that have a 5'-terminal phosphate anchor at a nick or a 1- or 2-nucleotide flap and is augmented by a 3' flap. Also has endonuclease activity toward 5'-flaps.
  • 基因功能参考文献：
    1. hFAN1 homodimerization plays a role in biological processes that involve 5' DNA Flap cleavage. PMID: 29518739
    2. FAN1 interaction with ubiquitylated PCNA alleviates replication stress and preserves genomic integrity independently of BRCA2 PMID: 29051491
    3. The structures, function, and proposed mechanisms of FAN1 nuclease are discussed, and the insights into its role in interstrand cross-links repair and in processing of stalled replication forks are provided. [REVIEW] PMID: 28623094
    4. show that DNA repair genes (fan1 and pms2) significantly modify age at onset in Huntington's Disease and Spinocerebellar Ataxias, suggesting a common pathogenic mechanism, which could operate through the observed somatic expansion of repeats PMID: 27044000
    5. FAN1 efficiently promoted DNA incision at the proper site of RPA-coated 5'-flapped DNA. Therefore, FAN1 possesses the ability to promote the ICL repair of 5'-flapped DNA covered by RPA. PMID: 25922199
    6. EXO1 and FEN1 cleaved the substrate at the boundary between the single-stranded 5' flap and the duplex, whereas FAN1 incised it three to four nucleotides in the double-stranded region. PMID: 26221031
    7. Detected FAN1 mutations in approximately 3% of families who met the Amsterdam criteria for hereditary colorectal cancer and had mismatch repair-proficient cancers with no previously associated mutations. PMID: 26052075
    8. The crystal structures of human FAN1 in complex with a 5' flap DNA substrate show that two FAN1 molecules form a head-to-tail dimer to locate the lesion, orient the DNA, and unwind a 5' flap for subsequent incision. PMID: 25500724
    9. In this work, FAN1-DNA crystal structures and biochemical data reveal that human FAN1 cleaves DNA successively at every third nucleotide PMID: 25430771
    10. Results show that FAN1 utilizes its nuclease activity-in cooperation with the BLM-FANCD2 complex-to promote replication fork restart and simultaneous suppression of new origin firing. PMID: 25135477
    11. FAN1 encodes a DNA repair enzyme, thus implicating abnormalities in DNA repair in the susceptibility to schizophrenia or autism PMID: 24344280
    12. FAN1 might be a new mitotic substrate of APC/CCdh1 that plays a key role during mitotic exit. PMID: 22854063
    13. By exome sequencing, we identified mutations in FAN1 as a cause of karyomegalic interstitial nephritis, a disorder that serves as a model for renal fibrosis. PMID: 22772369
    14. Our results suggest that FAN1 has a minor role in interstrand crosslink repair compared with true Fanconi anemia genes and exclude FAN1 as a novel FA gene. PMID: 22611161
    15. Two FAN1 variants do not appear to be causal for breast cancer. PMID: 21858661
    16. Data show that FAN1 in DT40 cells participates in the processing of damage induced by interstrand cross-linking-generating agents also independently of the classical FA pathway. PMID: 21115814
    17. Data show that FAN1 is a nuclear protein and forms DNA-damage-induced foci, which appear to be at stalled replication forks as denoted by RPA colocalization. PMID: 20935496
    18. study characterizes FANI which promotes DNA interstrand cross-linking repair in a manner strictly dependent on its ability to accumulate at or near sites of DNA damage and that relies on mono-ubiquitylation of the FANCI-FANCD2 complex PMID: 20671156
    19. Study describes how a highly conserved protein, KIAA1018/MTMR15/FAN1, that interacts with, and is recruited to sites of DNA damage by, the monoubiquitinated form of FANCD2. PMID: 20603015
    20. KIAA1018 is a 5'-->3' exonuclease and a structure-specific endonuclease that preferentially incises 5' flaps; like cells from FA patients, human cells depleted of KIAA1018 are sensitized to ICL-inducing agents and display chromosomal instability. PMID: 20603016
    21. FAN1 colocalizes at sites of DNA damage with the ID complex in a manner dependent on FAN1's ubiquitin-binding domain (UBZ), the FANCI-FAND2 (ID) complex, and monoubiquitination of FANCD2. PMID: 20603073

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  • 相关疾病：
    Interstitial nephritis, karyomegalic (KMIN)
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    FAN1 family
  • 数据库链接：

    HGNC: 29170

    OMIM: 613534

    KEGG: hsa:22909

    STRING: 9606.ENSP00000354497

    UniGene: Hs.584863