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GEMIN2 Antibody

  • 货号：
    CSB-PA021329LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate
      All lanes: GEMIN2 antibody at 3.7μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 32, 30, 29, 5 kDa
      Observed band size: 32 kDa
    • Immunofluorescence staining of Hela cells with CSB-PA021329LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunoprecipitating GEMIN2 in 293T whole cell lysate
      Lane 1: Rabbit control IgG instead of CSB-PA021329LA01HU in 293T whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000)
      Lane 2: CSB-PA021329LA01HU (6μg) + 293T whole cell lysate (0.5mg)
      Lane 3: 293T whole cell lysate (20μg)
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) GEMIN2 Polyclonal antibody
  • Uniprot No.：
    O14893
  • 基因名：
    GEMIN2
  • 别名：
    Component of gems 2 antibody; Gem (nuclear organelle) associated protein 2 antibody; Gem associated protein 2 antibody; GEMI2_HUMAN antibody; Gemin-2 antibody; gemin2 antibody; SIP 1 antibody; SIP-1 antibody; SIP1 antibody; SIP1 delta antibody; SIP1-delta antibody; SMN interacting protein 1 antibody; SMN interacting protein 1 delta antibody; SMN-interacting protein 1 antibody; Survival interacting protein 1 antibody; Survival of motor neuron protein interacting protein 1 antibody; Survival of motor neuron protein-interacting protein 1 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Gem-associated protein 2 protein (1-280AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，GEMIN2 Antibody (CSB-PA021329LA01HU)，的标记方式是Non-conjugated。对于GEMIN2 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA021329LB01HU GEMIN2 Antibody, HRP conjugated ELISA
    FITC CSB-PA021329LC01HU GEMIN2 Antibody, FITC conjugated
    Biotin CSB-PA021329LD01HU GEMIN2 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IF 1:50-1:200
    IP 1:200-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG (5Sm) are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A. Binding of snRNA inside 5Sm ultimately triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. Within the SMN complex, GEMIN2 constrains the conformation of 5Sm, thereby promoting 5Sm binding to snRNA containing the snRNP code (a nonameric Sm site and a 3'-adjacent stem-loop), thus preventing progression of assembly until a cognate substrate is bound.
  • 基因功能参考文献：
    1. Specific mutations in the yeast Sm protein ring expose a requirement for assembly factor Brr1, a homolog of human Gemin2. PMID: 27974620
    2. The s propose that Gemin2 is a versatile hub for ribonucleoprotein exchange that functions broadly in RNA metabolism. PMID: 26828962
    3. investigate the oligomeric nature of the SMN.Gemin2 complexes from humans and fission yeast (hSMN.Gemin2 and ySMN.Gemin2) PMID: 26092730
    4. Two monoclonal antibodies against SMN (survival-of-motor-neurons) protein bind to its site of interaction with gemin2. PMID: 23939045
    5. several conserved SMN residues, including the sites of two SMA patient mutations, are not required for binding to Gemin2. Instead, they form a conserved SMN/Gemin2 surface that may be functionally important for snRNP assembly. PMID: 22607171
    6. Overexpression of SIP1 and downregulation of E-cadherin is associated with delayed neck metastasis in stage I/II oral tongue squamous cell carcinoma after partial glossectomy PMID: 21913013
    7. purified SMN-GEMIN2 fusion protein enhanced the RAD51-mediated homologous pairing much more efficiently than GEMIN2 alone PMID: 21732698
    8. Study identified Gemin2 as the protein that binds a pentamer of Sm proteins comprised of SmD1/D2 and SmF/E/G; the crystal structure of this complex bound to SMN's Gemin2 binding domain to 2.5 A was determined. PMID: 21816274
    9. Novel monoclonal antibodies detect SIP1 in the cytoplasm of human cells from multiple tumor tissue arrays. PMID: 20515682
    10. This study demonstrated that miR-141 levels correlate inversely with SIP1 protein levels as well as cell migration and invasion of CRC cells; SIP1 was identified as a functional target of miR-141. PMID: 19830559
    11. This study supports the rationale for developing SIP1 as a potential therapeutic and diagnostic target for gliomas PMID: 19806322
    12. SIP1 appears to stabilize functional multimer forms of IN, thereby promoting the assembly of IN and RT on viral RNA to allow efficient reverse transcription, which is a prerequisite for efficient HIV-1 infection PMID: 19915660
    13. Spinal muscular atrophy and amyotropic lateral sclerosis patients have decreased SIP 1-alpha and increased SIP 1-beta expression levels compared to normal tissues. PMID: 11943600
    14. SIP1 determines the capacity for snRNP assembly: biochemical deficiency in spinal muscular atrophy. PMID: 15964810
    15. binds to HIV-1 integrase and facilitates viral cDNA synthesis and subsequent steps that precede integration in vivo PMID: 16731905
    16. Gemin2 plays an important role in small nuclear ribonucleoprotein assembly through the stabilization of the survival of motor neuron oligomer/complex via novel self-interaction PMID: 17308308
    17. relationship between the feature of gene mutations and single nucleotide polymorphisms of SIP1 gene and HSCR PMID: 18247312
    18. We found upregulation of mRNA for transcription factors Snail, Slug, Twist, and SIP1 in spindle cell carcinoma when compared to squamous cell carcinoma. PMID: 19381684

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  • 亚细胞定位：
    Nucleus, gem. Cytoplasm. Note=Localized in subnuclear structures next to coiled bodies, called gems, which are highly enriched in spliceosomal snRNPs. Also found in the cytoplasm.
  • 蛋白家族：
    Gemin-2 family
  • 数据库链接：

    HGNC: 10884

    OMIM: 602595

    KEGG: hsa:8487

    STRING: 9606.ENSP00000308533

    UniGene: Hs.652307