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  4. GPHN Antibody

GPHN Antibody

  • 货号：
    CSB-PA173468
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA173468(GPHN Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)
    • The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using CSB-PA173468(GPHN Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)
  • 其他：

产品详情

  • Uniprot No.：
    Q03555
  • 基因名：
    Gphn
  • 别名：
    Gphn antibody; GphGephyrin antibody; Putative glycine receptor-tubulin linker protein) [Includes: Molybdopterin adenylyltransferase antibody; MPT adenylyltransferase antibody; EC 2.7.7.75 antibody; Domain G); Molybdopterin molybdenumtransferase antibody; MPT Mo-transferase antibody; EC 2.10.1.1 antibody; Domain E)] antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthetic peptide of Human GPHN
  • 免疫原种属：
    Rattus norvegicus (Rat)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:2000-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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靶点详情

  • 功能：
    Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules. Acts as a major instructive molecule at inhibitory synapses, where it also clusters GABA type A receptors.; Has also a catalytic activity and catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.
  • 基因功能参考文献：
    1. endosomal pool of PI3P, generated by the class III phosphatidylinositol 3-kinase, is important for the Cb-mediated recruitment of gephyrin and GABAA receptors to developing inhibitory postsynapses and thus the formation of postsynaptic membrane specializations. PMID: 27941024
    2. these data reveal that IQSEC3 acts together with gephyrin to regulate inhibitory synapse development. PMID: 27002143
    3. CDK5 plays an essential role for the stability of gephyrin-dependent GABAA receptor clusters in hippocampal neurons. PMID: 25093719
    4. These results reveal a homeostatic mechanism through which activity regulates the dynamics and function of perisomatic inhibitory synapses, and they identify a CaMKII-dependent phosphorylation site on gephyrin. PMID: 25535349
    5. A rationale is provided for the role of the 150-residue linker in the overall structure and conformational dynamics of gephyrin. A high degree of flexibility is revealed in its structure, and both terminal domains can oligomerize in their isolated forms. PMID: 24100323
    6. The enhancement of Cb-induced gephyrin clustering by GTP-TC10 does not depend on the guanine nucleotide exchange activity of Cb but involves an interaction that resembles reported interactions of other small GTPases with their effectors PMID: 24297911
    7. Nanoscopic imaging based on single-molecule detections characterizes the ultrastructure of inhibitory synapses and counts the number of scaffold proteins and receptor binding sites. PMID: 23889935
    8. The changes in protein expression of gephyrin detected in these studies provide a potential mechanism to explain the deficits in GABAergic neurotransmission observed during the epileptogenic period PMID: 23294024
    9. Phosphorylation of gephyrin in hippocampal neurons by cyclin-dependent kinase CDK5 at Ser-270 is dependent on collybistin. PMID: 22778260
    10. There may be a correlation between the distribution density of gephyrin clusters in the submembrane region of ventromedial (Vm) motoneurons and that of axon terminals making inhibitory synapses on Vm motoneurons. PMID: 22290869
    11. We propose two models for the domain arrangement in hexameric gephyrin based on the oligomerization of either the E or C domains, with the latter being crucial for the regulation of gephyrin clustering. PMID: 22351777
    12. Gephyrin expression is involved in the development of temporal lobe epilepsy in an experimental rat model. PMID: 21404332
    13. clustering of GABA(A)R alpha2, alpha3, and GlyRs by gephyrin is mediated by distinct mechanisms at mixed glycinergic/GABAergic synapses. PMID: 21880742
    14. a key role of gephyrin in regulating transynaptic signaling at both inhibitory and excitatory synapses. PMID: 21507951
    15. Conditioned fear increased the number of cells co-expressing GABA-A receptor alpha-2 subunits and gephyrin in the basolateral amygdala and dentate gyrus of the hippocampus in high anxiety rats. PMID: 20833253
    16. Gephyrin is a synaptogenic molecule regulating GABAergic synaptic plasticity, and likely contributes to the therapeutic action of lithium. PMID: 21173228
    17. Data are consistent with the postsynaptic gephyrin scaffold acting as a platform for protein phosphatase 1 (PP1), which regulates gephyrin cluster size by dephosphorylation of gephyrin- or cytoskeleton-associated proteins. PMID: 20206270
    18. These results indicate that gephyrin is essential not only for maintaining synaptic GABA(A) receptor clusters in the right position but also for regulating both phasic and tonic inhibition. PMID: 19660531
    19. Gephyrin, profilin and Mena/VASP colocalize at rat spinal cord synapses, cultured neurons and in gephyrin clusters in transfected cells. Mena/VASP and profilin can contribute to postulated linkage between receptors, gephyrin scaffolds and microfilaments PMID: 12967995
    20. These results identify the C5 splice variant of gephyrin as a factor regulating the transmitter-appropriate degree of Glycine receptor accumulation at inhibitory synapses PMID: 14960612
    21. The structural basis of dynamic glycine receptor clustering by gphn was studied. PMID: 15201864
    22. Results implicate gephyrin in fear acquisition and extinction and suggest that modulation of gephyrin and GABA(A) receptor expression in the basolateral amygdala may play a role in the experience-dependent plasticity underlying these types of learning. PMID: 15647495
    23. Data suggest that ena/VASP binding to F-actin allows for gephyrin recruitment to the leading edge of uncapped actin filaments. PMID: 16376568
    24. The resolution crystal structure of the Cdc42-collybistin II complex reveals a novel conformation of the switch I region of Cdc42, and biochemical data indicate that gephyrin negatively regulates collybistin activity [collybistin II]. PMID: 16616186
    25. The expression of various gephyrin isoforms in rat brain, liver, and spinal cord is reported. PMID: 17001074
    26. These findings indicate that the expression of gephyrin and the density of cortical inhibitory synapses are not influenced by endogenous neurosteroids. PMID: 17698049
    27. It is concluded that gephyrin plays a critical role for the stability of GABAergic synapses. PMID: 17916433
    28. The levels of expression and clustering of gephyrin not only has homotypic effects on GABAergic synaptic contacts, but also has heterotypic effects on glutamatergic synaptic contacts. PMID: 18199120
    29. Data suggest that gephyrin alterations due to protein accumulation stress are reduced by the lysosomal modulator Z-Phe-Ala-diazomethylketone.( PMID: 18204977
    30. Expression of Gphn was studied in the cochlea. PMID: 18231803
    31. Clustering of gephyrin occurs in concert with that of GABA-A receptors, with striking differences in the temporal regulation of gephyrin at dendritic and somatic synapses of Purkinje cells. PMID: 18366064
    32. A significant increase in the inhibitory postsynaptic scaffold protein, gephyrin, was observed in the parietal cortex of aged cognitively impaired animals. PMID: 19105974
    33. crystal structure of N terminal G domain PMID: 11554796

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  • 亚细胞定位：
    Cell junction, synapse, postsynaptic cell membrane; Lipid-anchor; Cytoplasmic side. Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytosol. Cytoplasm, cytoskeleton. Cell projection, dendrite. Cell junction, synapse, postsynaptic density.
  • 蛋白家族：
    MoaB/Mog family; MoeA family
  • 组织特异性：
    Expressed in tissues including spinal cord, brain, liver, kidney and lung.
  • 数据库链接：

    KEGG: rno:64845

    UniGene: Rn.11032