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GSDMA Antibody

  • 货号：
    CSB-PA850415LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: HGC27 whole cell lysate
      All lanes: GSDMA antibody at 3.85µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 50 kDa
      Observed band size: 50 kDa
    • IHC image of CSB-PA850415LA01HU diluted at 1:400 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA850415LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品描述：

    The product CSB-PA850415LA01HU is an IgG rabbit polyclonal GSDMA antibody suitable for the recognition of human GSDMA by ELISA, WB, IHC, and IF. The recombinant human GSDMA protein (64-172aa) is used to immunize the rabbit to generate the GSDMA polyclonal antibody. GSDMA antibody is available in the non-conjugated IgG antibody form. Its purity is over 95% using protein G purification.

    The GSDMA protein act as a regulator of cell death and inflammation. GSDMA is primarily expressed in epithelial tissues, where it has been shown to play a critical role in regulating immune responses and tissue homeostasis. It also participates in various physiological processes, including skin development and maintenance of the skin barrier function. Dysregulation of GSDMA causes skin disorders, inflammatory bowel disease, cancer, and other diseases.

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) GSDMA Polyclonal antibody
  • Uniprot No.：
    Q96QA5
  • 基因名：
  • 别名：
    GSDMA antibody; GSDM antibody; GSDM1 antibody; FKSG9Gasdermin-A antibody; Gasdermin-1 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Gasdermin-A protein (64-172AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，GSDMA Antibody (CSB-PA850415LA01HU)，的标记方式是Non-conjugated。对于GSDMA Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA850415LB01HU GSDMA Antibody, HRP conjugated ELISA
    FITC CSB-PA850415LC01HU GSDMA Antibody, FITC conjugated
    Biotin CSB-PA850415LD01HU GSDMA Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    This form constitutes the precursor of the pore-forming protein: upon cleavage, the released N-terminal moiety (Gasdermin-A, N-terminal) binds to membranes and forms pores, triggering cell death.; Pore-forming protein that causes membrane permeabilization and pyroptosis. Released upon cleavage in vitro of genetically engineered GSDMA, and binds to membrane inner leaflet lipids. Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, triggering pyroptosis. Binds to membrane inner leaflet lipids, such as phosphatidylinositol (4,5)-bisphosphate. The functional mechanisms and physiological proteases that cleave and activate this pore-forming protein are unknown.
  • 基因功能参考文献：
    1. modest changes (8-13%) in promoter methylation levels of ZPBP2 and GSDMA may cause substantial changes in RNA levels and allelic expression of ZPBP2 and ORMDL3 is mediated by DNA methylation PMID: 28241063
    2. GSDMA was identified as a susceptibility gene for systemic sclerosis. PMID: 28314753
    3. we investigated the association between GSDMA and GSDMB variants and the incidence of adult and childhood asthma among Jordanians.An association between the GSDMB T/C single nucleotide polymorphism (SNP) genotype and the incidence of childhood asthma was found PMID: 26886240
    4. The local genotype influences methylation levels at SLC22A5 and ZPBP2 promoters independently of the asthma status. Further studies are necessary to confirm the relationship between GSDMA-ZPBP2 and SLC22A5 methylation and asthma in females and males separately. PMID: 26671913
    5. Based on our results and published findings on GSDMA, GSDMB, LRRC3C, and related proteins, we propose that this locus in part affects IBD susceptibility via effects on apoptosis and cell proliferation PMID: 26484354
    6. Study identified significantly white blood cell count (WBC) level associated SNPs of three separate genes GSDMA, MED24, and PSMD3 in European continent (EA) subjects. PMID: 22037903
    7. The GSDMA (rs7212938) and GSDMB (rs7216389) polymorphisms are associated with asthma susceptibility and intermediate asthma phenotypes. PMID: 21337730
    8. Results suggest an association of 17q21 polymorphisms with ORMDL3, GSDMA expression, and IL-17 secretion early in life. These observations may imply a functional role of the 17q21 locus affecting T-cell development during immune maturation. PMID: 21546069
    9. The present data suggest that TGF-beta, LMO1, possibly RUNX3, and GSDM form a regulatory pathway for directing the pit cells to apoptosis. PMID: 17471240
    10. Study investigated the expression pattern of the GSDM family genes in the upper gastrointestinal epithelium and cancers. PMID: 19051310
    11. Evolutionary recombination hotspot around the GSDML-GSDM locus is closely linked to oncogenomic recombination hotspot around the PPP1R1B-STARD3-TCAP-PNMT-PERLD1-ERBB2-C17orf37-GRB7 amplicon at human chromosome 17q12. PMID: 15010812

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  • 亚细胞定位：
    [Gasdermin-A]: Cytoplasm, perinuclear region. Cytoplasm, cytosol.; [Gasdermin-A, N-terminal]: Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Gasdermin family
  • 组织特异性：
    Expressed predominantly in the gastrointestinal tract and, at a lower level, in the skin. Also detected in mammary gland. In the gastrointestinal tract, mainly expressed in differentiated cells, including the differentiated cell layer of esophagus and muc
  • 数据库链接：

    HGNC: 13311

    OMIM: 611218

    KEGG: hsa:284110

    STRING: 9606.ENSP00000301659

    UniGene: Hs.448873