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HMGCS2 Antibody

  • 货号：
    CSB-PA010567LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Rat kidney tissue
      All lanes: HMGCS2 antibody at 4.5μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 57, 53, 51 kDa
      Observed band size: 57 kDa
    • IHC image of CSB-PA010567LA01HU diluted at 1:400 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA010567LA01HU diluted at 1:400 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA010567LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) HMGCS2 Polyclonal antibody
  • Uniprot No.：
    P54868
  • 基因名：
  • 别名：
    3 hydroxy 3 methylglutaryl Coenzyme A synthase 2 (mitochondrial) antibody; 3 hydroxy 3 methylglutaryl Coenzyme A synthase 2 antibody; 3 hydroxy 3 methylglutaryl coenzyme A synthase antibody; 3-hydroxy-3-methylglutaryl coenzyme A synthase antibody; HMCS2_HUMAN antibody; HMG CoA synthase antibody; HMG-CoA synthase antibody; HMGCS 2 antibody; HMGCS2 antibody; Hydroxymethylglutaryl CoA synthase antibody; Hydroxymethylglutaryl CoA synthase mitochondrial antibody; Hydroxymethylglutaryl-CoA synthase antibody; mitochondrial antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Rat
  • 免疫原：
    Recombinant Human Hydroxymethylglutaryl-CoA synthase, mitochondrial protein (426-508AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，HMGCS2 Antibody (CSB-PA010567LA01HU)，的标记方式是Non-conjugated。对于HMGCS2 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA010567LB01HU HMGCS2 Antibody, HRP conjugated ELISA
    FITC CSB-PA010567LC01HU HMGCS2 Antibody, FITC conjugated
    Biotin CSB-PA010567LD01HU HMGCS2 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Catalyzes the first irreversible step in ketogenesis, condensing acetyl-CoA to acetoacetyl-CoA to form HMG-CoA, which is converted by HMG-CoA reductase (HMGCR) into mevalonate.
  • 基因功能参考文献：
    1. several nutrients as well as specific nutritional related hormonal conditions are able to affect significantly HMGCS2 gene expression. PMID: 28888048
    2. miR-107-mediated decrease of HMGCS2 indicates poor outcomes and promotes cell migration in hepatocellular carcinoma PMID: 28867541
    3. this studies demonstrate that HMGCS2 functions as a transcriptional factor that binds to peroxisome proliferator-activated receptor alpha (PPARalpha), resulting in Src expression and activation in a metabolically independent manner PMID: 27816970
    4. HMGCS2 shares with ketone bodies common features in autophagic clearance of APP, suggesting that ketone bodies play an important role in HMGCS2 regulation of the autophagy. PMID: 28320515
    5. High expression of either HSD17B2 or HMGCS2 predicted poor susceptibility of rectal cancer to preoperative chemoradiotherapy. PMID: 25929810
    6. FABP7 and HMGCS2 may have roles in apocrine differentiation categorizing apocrine carcinoma of the breast PMID: 25389781
    7. In Fe-S cluster-deficient muscles, results show a dramatic up-regulation of the ketogenic enzyme HMGCS2 and the secreted protein FGF21 (fibroblast growth factor 21). PMID: 23943793
    8. Human HMGCS2 regulates mitochondrial fatty acid oxidation and FGF21 expression PMID: 21502324
    9. Ketogenesis is an undesirable metabolic characteristic of the proliferating cell, which is down-regulated through c-Myc-mediated repression of the key metabolic gene HMGCS2. PMID: 16940161

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  • 相关疾病：
    3-hydroxy-3-methylglutaryl-CoA synthase-2 deficiency (HMGCS2D)
  • 亚细胞定位：
    Mitochondrion.
  • 蛋白家族：
    HMG-CoA synthase family
  • 组织特异性：
    Expression in liver is 200-fold higher than in any other tissue. Low expression in colon, kidney, testis, and pancreas. Very low expression in heart and skeletal muscle. Not detected in brain.; [Isoform 3]: Highest expression detected in heart and skeleta
  • 数据库链接：

    HGNC: 5008

    OMIM: 600234

    KEGG: hsa:3158

    STRING: 9606.ENSP00000358414

    UniGene: Hs.59889