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HOOK3 Antibody

  • 货号：
    CSB-PA769790LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: 293 whole cell lysate
      All lanes: HOOK3 antibody at 4.9µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 84 kDa
      Observed band size: 84 kDa
    • IHC image of CSB-PA769790LA01HU diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunoprecipitating HOOK3 in 293 whole cell lysate
      Lane 1: Rabbit control IgG instead of CSB-PA769790LA01HU in 293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-PA769790LA01HU (6µg) + 293 whole cell lysate (500µg)
      Lane 3: 293 whole cell lysate (20µg)
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) HOOK3 Polyclonal antibody
  • Uniprot No.：
    Q86VS8
  • 基因名：
    HOOK3
  • 别名：
    FLJ31058 antibody; h-hook3 antibody; hHK3 antibody; HK3 antibody; hook3 antibody; HOOK3_HUMAN antibody; OTTHUMP00000229200 antibody; Protein Hook homolog 3 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Protein Hook homolog 3 protein (357-455AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，HOOK3 Antibody (CSB-PA769790LA01HU)，的标记方式是Non-conjugated。对于HOOK3 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA769790LB01HU HOOK3 Antibody, HRP conjugated ELISA
    FITC CSB-PA769790LC01HU HOOK3 Antibody, FITC conjugated
    Biotin CSB-PA769790LD01HU HOOK3 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IP 1:200-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Probably serves as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking. Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). FHF complex promotes the distribution of AP-4 complex to the perinuclear area of the cell.; (Microbial infection) May serve as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking.
  • 基因功能参考文献：
    1. This work reveals the structural details of Hook3's interaction with dynein and offers insight into how cargo adaptors form processive dynein-dynactin motor complexes. PMID: 27482052
    2. Single-molecule motility assays using total internal reflection fluorescence microscopy indicate that both Hook1 and Hook3 effectively activate cytoplasmic dynein, inducing longer run lengths and higher velocities than the previously characterized dynein activator bicaudal D2 (BICD2). PMID: 27365401
    3. results of our study identify HOOK3 as a strong candidate prognostic marker with a possible role in maintaining genomic integrity in prostate cancer, which may have potential for inclusion into clinical routine assays PMID: 26230842
    4. Results found that downregulation of Hook3 expression slows down endosomal transport and increases beta-amyloid production suggesting that Hook3 might play a critical role in pathogenic events exacerbating Alzheimer disease. PMID: 25799409
    5. Hook3 is recruited to pericentriolar satellites through interaction with pericentriolar material 1 protein PCM1. PMID: 20152126
    6. Salmonella SpiC targets the function of Hook3, a mammalian protein implicated in cellular trafficking. PMID: 12950921
    7. Hook3 interacts with a cytoplasmic domain of scavenger receptor A PMID: 17237231
    8. A fusion product between exon 11 of HOOK3 and exon 12 of RET gene was identified by 5'RACE, and the presence of chimeric HOOK3-RET protein of 88 kDa was detected by western blot analysis with an anti-RET antibody. PMID: 17639057

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Golgi apparatus.
  • 蛋白家族：
    Hook family
  • 数据库链接：

    HGNC: 23576

    OMIM: 607825

    KEGG: hsa:84376

    STRING: 9606.ENSP00000305699

    UniGene: Hs.162852