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IDH3A Antibody

  • 货号：
    CSB-PA010991LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: HepG2 whole cell lysat, Jurkat whole cell lysate, Rat brain tissue, Mouse brain tissue, Mouse liver tissue
      All lanes: IDH3A antibody at 3.4µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 40, 32 kDa
      Observed band size: 40 kDa
    • Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA010991LA01HU at dilution of 1:100
    • Immunofluorescent analysis of A549 cells using CSB-PA010991LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
    • Immunoprecipitating IDH3A in HepG2 whole cell lysate
      Lane 1: Rabbit control IgG instead of CSB-PA010991LA01HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-PA010991LA01HU (6µg) + HepG2 whole cell lysate (500µg)
      Lane 3: HepG2 whole cell lysate (20µg)
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) IDH3A Polyclonal antibody
  • Uniprot No.：
    P50213
  • 基因名：
    IDH3A
  • 别名：
    H IDH alpha antibody; HIDH alpha antibody; IDH3A antibody; IDH3A_HUMAN antibody; Isocitrate dehydrogenase (NAD+) alpha chain precursor antibody; Isocitrate dehydrogenase [NAD] subunit alpha antibody; Isocitrate dehydrogenase [NAD] subunit alpha mitochondrial antibody; Isocitrate dehydrogenase 3 (NAD+) alpha antibody; Isocitrate dehydrogenase antibody; Isocitric dehydrogenase antibody; Isocitric dehydrogenase subunit alpha antibody; mitochondrial antibody; NAD antibody; NAD(+)-specific ICDH subunit alpha antibody; NAD(H) specific isocitrate dehydrogenase alpha subunit precursor antibody; NAD+ specific ICDH antibody; NADsubunit alpha mitochondrial precursor antibody; OTTHUMP00000184906 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse, Rat
  • 免疫原：
    Recombinant Human Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial protein (28-108AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，IDH3A Antibody (CSB-PA010991LA01HU)，的标记方式是Non-conjugated。对于IDH3A Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA010991LB01HU IDH3A Antibody, HRP conjugated ELISA
    FITC CSB-PA010991LC01HU IDH3A Antibody, FITC conjugated
    Biotin CSB-PA010991LD01HU IDH3A Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:20-1:200
    IF 1:50-1:200
    IP 1:200-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Catalytic subunit of the enzyme which catalyzes the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.
  • 基因功能参考文献：
    1. The silencing of IDH3a significantly delayed tumor growth by suppressing the HIF-1-mediated Warburg effect and angiogenesis. PMID: 25531325
    2. for IDH, a normal alpha subunit is required for catalytic activity and alpha-Arg88 likely participates in the isocitrate site, whereas the beta and gamma subunits have roles in the nucleotide functions of this allosteric enzyme PMID: 14555658
    3. active sites of the human NAD-IDH are shared between alpha and gamma subunits and between alpha and beta subunits PMID: 16737955
    4. Asp181 is essential for nicotinamide-adenine dinucleotide (NAD)-specific IDH alpha subunit catalysis and may also facilitate the binding of substrates. PMID: 17432878
  • 亚细胞定位：
    Mitochondrion.
  • 蛋白家族：
    Isocitrate and isopropylmalate dehydrogenases family
  • 数据库链接：

    HGNC: 5384

    OMIM: 601149

    KEGG: hsa:3419

    STRING: 9606.ENSP00000299518

    UniGene: Hs.591110