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KHK Antibody

  • 货号：
    CSB-PA012157ESR1HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western blot
      All lanes: KHKantibody at 3.6μg/ml
      Lane 1: Mouse liver tissue
      Lane 2: Mouse kidney tissue
      Lane 3: Rat kidney tissue
      Secondary
      Goat polyclonal to rabbit IgG at 1/10000 dilution
      Predicted band size: 33 kDa
      Observed band size: 33 kDa
    • Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA012157ESR1HU at dilution of 1:100
    • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA012157ESR1HU at dilution of 1:100
    • Immunoprecipitating KHK in Mouse liver tissue
      Lane 1: Rabbit control IgG instead of CSB-PA012157ESR1HU in Mouse liver tissue. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-PA012157ESR1HU (8μg) + Mouse liver tissue (500μg)
      Lane 3: Mouse liver tissue (10μg)
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) KHK Polyclonal antibody
  • Uniprot No.：
    P50053
  • 基因名：
    KHK
  • 别名：
    EC 2.7.1.3 antibody; Fructokinase antibody; Hepatic fructokinase antibody; Ketohexokinase antibody; Ketohexokinase isoform a antibody; KHK antibody; KHK_HUMAN antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse, Rat
  • 免疫原：
    Recombinant Human Ketohexokinase protein (1-298AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:20-1:200
    IP 1:200-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Catalyzes the phosphorylation of the ketose sugar fructose to fructose-1-phosphate.
  • 基因功能参考文献：
    1. Data indicate metabolic enzymes NAD kinase and ketohexokinase as candidate metabolic gene targets, and the chromatin remodeling protein INO80C as a tumor suppressor in KRAS(MUT) colorectal tumor xenograft. PMID: 28954733
    2. Angelica archangelica, Garcinia mangostana, Petroselinum crispum, and Scutellaria baicalensis were the top four botanical candidiates identified with inhibitory activity against ketohexokinase-C. PMID: 27322374
    3. compared with normal hepatocytes, hepatocellular carcinoma (HCC) cells markedly reduce the rate of fructose metabolism and the level of reactive oxygen species, as a result of a c-Myc-dependent and heterogeneous nuclear ribonucleoprotein (hnRNP) H1- and H2-mediated switch from expression of the high-activity fructokinase (KHK)-C to the low-activity KHK-A isoform. PMID: 27088854
    4. myocardial hypoxia actuates fructose metabolism in human and mouse models of pathological cardiac hypertrophy through hypoxia-inducible factor 1alpha (HIF1alpha) activation of SF3B1 and SF3B1-mediated splice switching of KHK-A to KHK-C PMID: 26083752
    5. These studies identify fructokinase as a novel mediator of diabetic nephropathy and document a novel role for endogenous fructose production, or fructoneogenesis, in driving renal disease. PMID: 24876114
    6. This study determined if single nucleotide polymorphisms in genes involved in fructose transport,SLC2A2 and SLC2A5 and metabolism, etohexokinase affect inter-individual variability in metabolic phenotypes. PMID: 23341889
    7. In human hepatocytes uric acid up-regulates KHK expression thus leading to the amplification of the lipogenic effects of fructose. PMID: 23112875
    8. ketohexokinase-A serves an unknown physiologic function that remains intact in essential fructosuria. PMID: 12941785
    9. The expression of ketohexokinase is diminished in human clear cell type of renal cell carcinoma PMID: 16372272
    10. Ketohexokinase-dependent metabolism of fructose induces proinflammatory mediators in proximal tubular cells. PMID: 19158351
    11. The structure of the KHK-A ternary complex revealed an active site with fructose & the ATP analogue in positions ready for phosphorylation. The effects of the pathogenic mutations Gly40Arg & Ala43Thr have been modelled in the context of the KHK structure. PMID: 19237742

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  • 相关疾病：
    Fructosuria (FRUCT)
  • 蛋白家族：
    Carbohydrate kinase PfkB family
  • 组织特异性：
    Most abundant in liver, kidney, gut, spleen and pancreas. Low levels also found in adrenal, muscle, brain and eye.
  • 数据库链接：

    HGNC: 6315

    OMIM: 229800

    KEGG: hsa:3795

    STRING: 9606.ENSP00000260598

    UniGene: Hs.567297