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KRT16 Antibody

  • 货号：
    CSB-PA012515LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate
      All lanes: KRT16 antibody at 3.4μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 52 kDa
      Observed band size: 52 kDa
    • IHC image of CSB-PA012515LA01HU diluted at 1:400 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA012515LA01HU diluted at 1:400 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of MCF-7 cells with CSB-PA012515LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) KRT16 Polyclonal antibody
  • Uniprot No.：
    P08779
  • 基因名：
  • 别名：
    CK 16 antibody; CK-16 antibody; CK16 antibody; Cytokeratin 16 antibody; Cytokeratin-16 antibody; Cytokeratin16 antibody; FNEPPK antibody; Focal non epidermolytic palmoplantar keratoderma antibody; K 16 antibody; K16 antibody; K1C16_HUMAN antibody; K1CP antibody; Keratin 1 type I antibody; Keratin 16 antibody; Keratin antibody; Keratin type I cytoskeletal 16 antibody; Keratin-16 antibody; Keratin16 antibody; KRT 16 antibody; Krt16 antibody; KRT16A antibody; NEPPK antibody; PC1 antibody; type I cytoskeletal 16 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Keratin, type I cytoskeletal 16 protein (265-333AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，KRT16 Antibody (CSB-PA012515LA01HU)，的标记方式是Non-conjugated。对于KRT16 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA012515LB01HU KRT16 Antibody, HRP conjugated ELISA
    FITC CSB-PA012515LC01HU KRT16 Antibody, FITC conjugated
    Biotin CSB-PA012515LD01HU KRT16 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance.
  • 基因功能参考文献：
    1. Study identified iRHOM2 as a novel regulator of K16 in humans and mice, with important implications for palmoplantar keratodermas, wound healing, inflammatory skin disease and cancers. PMID: 28128203
    2. results suggest a conditional regulation of KRT16 gene by ATF4 that may be inhibited in normal cells, but engaged during cancer progression. Potential roles of KRT16, FAM129A and HKDC1 genes upregulation in adaptive stress responses and pathologies are discussed PMID: 29420561
    3. inflammatory cytokines promoted Nrf2 nuclear translocation in psoriatic epidermis, which led to elevated expression of K6, K16, and K17, thus promoting keratinocyte proliferation and contributing to the pathogenesis of psoriasis. PMID: 28576737
    4. The wider spectrum of KRT16 mutations suggests that changes in codons 125, 127, and 132 are most commonly responsible for PC-1 and that proline substitution mutations at codons 127 or 128 may produce more severe disease PMID: 24357266
    5. analysis of a new p.Leu421Pro (c.1262T>C) mutation in the highly conserved helix termination motif of K16 in a large Spanish family that may have a role in pachyonychia congenita PMID: 24118415
    6. Keratin 16 regulates innate immunity in response to epidermal barrier breach. PMID: 24218583
    7. Data established a seven-gene (AR, ESR2, GATA3, GBX2, KRT16, MMP28 and WNT11) prognostic signature to define a subset of triple-negative breast cancer (TNBC). PMID: 23549873
    8. Patients with p.Asn125Asp and p.Arg127Pro mutations in KRT16 exhibited more severe disease than patients carrying p.Asn125Ser and p.Arg127Cys mutations in terms of age of onset of symptoms, extent of nail involvement, and impact on daily quality of life. PMID: 21160496
    9. Data suggest that an immune response to trichohyalin and K16 may have a role in the pathogenesis of the enigmatic disorder. PMID: 20722389
    10. coinheritance of mutations in KRT16 and filaggrin may aggravate the pachyonychia congenita phenotype PMID: 19785597
    11. disease-associated keratin 16 expression is induced by epidermal growth factor is regulated through cooperation of transcription factors Sp1 and c-Jun PMID: 12954631
    12. K16 expression is observed in non-lesional psoriatic skin and may serve as a marker of preclinical psoriasis. PMID: 15239676
    13. ERK2-mediated C-terminal serine phosphorylation of p300 was a key event in the regulation of EGF-induced keratin 16 expression. PMID: 17623675
    14. DPPIV-expression and enzyme activity, Ki-67 antigen and K16 are significantly upregulated in the centre and inner margin of the psoriatic lesion compared to clinically uninvolved skin and the healthy volunteers skin. PMID: 18496701
    15. Antikeratin 16 autoAbs are involved in psoriasis, by exaggerating the innate immune response of keratinocytes. PMID: 18557933
    16. Infection by HPV stimulates the expression of K16 PMID: 19515043

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  • 相关疾病：
    Pachyonychia congenita 1 (PC1); Keratoderma, palmoplantar, non-epidermolytic, focal 1 (FNEPPK1)
  • 蛋白家族：
    Intermediate filament family
  • 组织特异性：
    Expressed in the corneal epithelium (at protein level).
  • 数据库链接：

    HGNC: 6423

    OMIM: 148067

    KEGG: hsa:3868

    STRING: 9606.ENSP00000301653

    UniGene: Hs.655160