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Krt18 Antibody

  • 货号：
    CSB-PA012519LA01MO
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, Mouse kidney tissue, Mouse liver tissue
      All lanes: Krt18 antibody at 5.5µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 48 kDa
      Observed band size: 48 kDa
    • IHC image of CSB-PA012519LA01MO diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA012519LA01MO diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Mus musculus (Mouse) Krt18 Polyclonal antibody
  • Uniprot No.：
    P05784
  • 基因名：
  • 别名：
    Krt18 antibody; Kerd antibody; Krt1-18 antibody; Keratin antibody; type I cytoskeletal 18 antibody; Cytokeratin endo B antibody; Keratin D antibody; Cytokeratin-18 antibody; CK-18 antibody; Keratin-18 antibody; K18 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Mouse, Human
  • 免疫原：
    Recombinant Mouse Keratin, type I cytoskeletal 18 protein (200-423AA)
  • 免疫原种属：
    Mus musculus (Mouse)
  • 标记方式：
    Non-conjugated

    本页面中的产品，Krt18 Antibody (CSB-PA012519LA01MO)，的标记方式是Non-conjugated。对于Krt18 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA012519LB01MO Krt18 Antibody, HRP conjugated ELISA
    FITC CSB-PA012519LC01MO Krt18 Antibody, FITC conjugated
    Biotin CSB-PA012519LD01MO Krt18 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • 基因功能参考文献：
    1. K18 deficiency of hepatocytes leads to steatosis, increasing with age, and finally to SH. K18 deficiency and age promote liver tumor development in mice, frequently on the basis of chromosomal instability, resembling human HCC with stemness features. PMID: 27689336
    2. K8/K18-dependent PKCdelta- and ASMase-mediated modulation of lipid raft size can explain the more prominent FasR-mediated signaling resulting from K8/K18 loss. PMID: 27422101
    3. autoantibodies were detected in aging K18-null male mice that had a related liver phenotype but normal colon compared with K8-null mice, suggesting that the autoantibodies are linked to liver rather than colonic disease PMID: 26399787
    4. In human failing myocardium, where TNF-alpha expression is upregulated, K8/K18 were also ectopically expressed PMID: 26280121
    5. The findings demonstrate the near complete loss of K8/K18 with concomitant high levels of vimentin in CT26 cells, a chemically-induced mouse colonic tumor. PMID: 25882495
    6. in contrast to its importance as luminal cell marker, CK18 is dispensable for the prostate morphogenesis but contributes to adult prostate regeneration PMID: 24672777
    7. Keratin 18 increases CFTR expression by binding to its C-terminal hydrophobic domain. PMID: 23045527
    8. Oxidative stress, Nrf2 and keratin 18 up-regulation associate with Mallory-Denk body formation in mouse erythropoietic protoporphyria PMID: 22334478
    9. Keratin 8 and keratin 18 mice on the same genetic background show similar sensitivity to DDC intoxication. PMID: 22449798
    10. These findings reveal the potential for cytokeratin 18 to be used as a diagnostic marker for early detection of hepatosplenic schistosomiasis. PMID: 21357724
    11. K18 glycosylation provides a unique protective role in epithelial injury by promoting the phosphorylation and activation of cell-survival kinases. PMID: 20729838
    12. Krt18 may be a useful immunohistochemical marker for detecting hepatocellular proliferative lesions in mice. PMID: 20035116
    13. Keratin 8 and keratin 18 play roles in regulating the shape and function of mitochondria. PMID: 19825937
    14. Data show that inhibition of extracellular signal-regulated kinase 1 and 2 (ERK1/2) activation sensitizes wild-type but not keratin 8 (K8)-null mouse hepatocytes to apoptosis, and is associated with a reduction in c-Flip protein, part of a K8/K18 complex. PMID: 15282307
    15. loss of the keratin cytoskeleton causes defects in the trophoblast giant cell layer PMID: 15819413
    16. K18 overexpression protects mice from Mallory body (MB) formation and from DDC-induced liver injury, which supports the importance of the K8-to-K18 ratio in MB formation. PMID: 17187412
    17. Study demonstrates here that, in the developing placenta of the mouse, the absence of the Mrj co-chaperone prevents proteasome degradation of keratin 18 intermediate filaments, resulting in the formation of keratin inclusion bodies. PMID: 17409114
    18. study shows that dominant mutation hK18 R89C, leads to cell type-specific lethality in mice, depending on ratio of mutant to endogenous keratins; a single, endogenous K18 allele rescued embryonic lethality but caused aggregation of keratins PMID: 17617404
    19. Keratin absence or mutation is well tolerated after pancreatic but not liver injury, whereas excessive overexpression is toxic to the pancreas but not the liver when induced under basal conditions. PMID: 18349119
    20. Over expression of K18 R89C predisposes transgenic mice to thioacetamide- but not CCl(4)-induced liver fibrosis. PMID: 18395095
    21. gene expression of krt8, krt18, and krt19 and correlation with gene expression profiles and cell differentiation are compared in human and mouse embryonic stem cells PMID: 18941637
    22. Results suggest that the K8/K18 loss induces a switch in Fas-induced death signaling, likely through a DEDD involvement. PMID: 19002587
    23. Resutls show that the appearance caspase-cleaved CK18 in plasma was found to reflect formation of the caspase-cleaved epitope in PMID: 19407366

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  • 亚细胞定位：
    Nucleus matrix. Nucleus, nucleolus. Cytoplasm.
  • 蛋白家族：
    Intermediate filament family
  • 组织特异性：
    Expressed in endoderm, intestinal epithelial cells and in most extraembryonic tissues.
  • 数据库链接：

    KEGG: mmu:16668

    STRING: 10090.ENSMUSP00000023803

    UniGene: Mm.22479