Your Good Partner in Biology Research

  1. Home
  2. 抗体
  3. 多克隆抗体
  4. LEO1 Antibody

LEO1 Antibody

  • 货号：
    CSB-PA845150NA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA845150NA01HU diluted at 1:300 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Western Blot
      Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate
      All lanes: LEO1 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 76, 69 kDa
      Observed band size: 76 kDa
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) LEO1 Polyclonal antibody
  • Uniprot No.：
    Q8WVC0
  • 基因名：
    LEO1
  • 别名：
    Gm185 antibody; LEO1 antibody; LEO1_HUMAN antibody; LOC123439 antibody; Paf1/RNA polymerase II complex component antibody; Replicative senescence down regulated leo1 like protein antibody; Replicative senescence down-regulated leo1-like protein antibody; Replicative senescence downregulated leo1 like antibody; RNA polymerase associated protein LEO1 antibody; RNA polymerase-associated protein LEO1 antibody; Senescence downregulated leo1 like antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Peptide sequence from Human RNA polymerase-associated protein LEO1 protein (2-24AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，LEO1 Antibody (CSB-PA845150NA01HU)，的标记方式是Non-conjugated。对于LEO1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA845150NB01HU LEO1 Antibody, HRP conjugated ELISA
    FITC CSB-PA845150NC01HU LEO1 Antibody, FITC conjugated
    Biotin CSB-PA845150ND01HU LEO1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:200-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both independently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Involved in polyadenylation of mRNA precursors. Connects PAF1C to Wnt signaling.
  • 基因功能参考文献：
    1. PRL-3 and Leo1 levels were positively associated in AML patient samples. PMID: 24686170
    2. Screened glioblastomas and oligodendrogliomas for fusion genes by identifying aberrant 5'-3' expression of genes that lie over regions of a copy number change. A fusion gene between exon 11 of LEO1 and exon 10 of SLC12A1 was identified. PMID: 20196086
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    LEO1 family
  • 组织特异性：
    Highly expressed in skeletal muscle and heart. Weakly expressed in placenta and liver.
  • 数据库链接：

    HGNC: 30401

    OMIM: 610507

    KEGG: hsa:123169

    STRING: 9606.ENSP00000299601

    UniGene: Hs.567662