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LYAR Antibody

  • 中文名称：
    LYAR兔多克隆抗体
  • 货号：
    CSB-PA882130LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: K562 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, PC-3 whole cell lysate
      All lanes: LYAR antibody at 2.5μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 44 kDa
      Observed band size: 44 kDa
    • IHC image of CSB-PA882130LA01HU diluted at 1:500 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA882130LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) LYAR Polyclonal antibody
  • Uniprot No.：
    Q9NX58
  • 基因名：
    LYAR
  • 别名：
    Cell growth regulating nucleolar protein antibody; Cell growth-regulating nucleolar protein antibody; Likely ortholog of mouse Ly1 antibody reactive clone antibody; Ly1 antibody reactive antibody; Ly1 antibody reactive homolog (mouse) antibody; Ly1 antibody reactive homolog antibody; LYAR antibody; LYAR_HUMAN antibody; ZC2HC2 antibody; ZLYAR antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Cell growth-regulating nucleolar protein (1-379AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，LYAR Antibody (CSB-PA882130LA01HU)，的标记方式是Non-conjugated。对于LYAR Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA882130LB01HU LYAR Antibody, HRP conjugated ELISA
    FITC CSB-PA882130LC01HU LYAR Antibody, FITC conjugated
    Biotin CSB-PA882130LD01HU LYAR Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:500-1:1000
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Plays a role in the maintenance of the appropriate processing of 47S/45S pre-rRNA to 32S/30S pre-rRNAs and their subsequent processing to produce 18S and 28S rRNAs. Also acts at the level of transcription regulation. Along with PRMT5, binds the gamma-globin (HBG1/HBG2) promoter and represses its expression. In neuroblastoma cells, may also repress the expression of oxidative stress genes, including CHAC1, HMOX1, SLC7A11, ULBP1 and SNORD41 that encodes a small nucleolar RNA. Preferentially binds to a DNA motif containing 5'-GGTTAT-3'. Negatively regulates the antiviral innate immune response by targeting IRF3 and impairing its DNA-binding activity. In addition, inhibits NF-kappa-B-mediated expression of proinflammatory cytokines. Stimulates phagocytosis of photoreceptor outer segments by retinal pigment epithelial cells. Prevents nucleolin/NCL self-cleavage, maintaining a normal steady-state level of NCL protein in undifferentiated embryonic stem cells (ESCs), which in turn is essential for ESC self-renewal.
  • 基因功能参考文献：
    1. Data suggest that LYAR induces proliferation and promotes survival of neuroblastoma cells by repressing the expression of oxidative stress genes such as CHAC1 and suppressing oxidative stress, and identify LYAR as a novel co-factor in N-Myc oncogenesis. PMID: 28686580
    2. LYAR may promote tumor cell migration and invasion by upregulating galectin-1 gene expression in colorectal cancer. PMID: 26413750
    3. Results show that LYAR protein expression is testis-predominant but can also be found in cancer cells originated from tissues other than testis. Lyar seems to promote proliferation of NIH-3T3 cells. PMID: 24990247
    4. The data indicate that LYAR acts as a novel transcription factor that binds the gamma-globin gene, and is essential for silencing the gamma-globin gene. PMID: 25092918
    5. Over-expression of LYAR increases cell proliferation without affecting the expression of c-Myc or p53. Some rapidly growing cells enhance ribosome biogenesis by increasing the expression of LYAR. PMID: 24495227

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  • 亚细胞定位：
    Nucleus. Nucleus, nucleolus. Cytoplasm. Cell projection, cilium, photoreceptor outer segment.
  • 组织特异性：
    Predominantly expressed in testis.
  • 数据库链接：

    HGNC: 26021

    OMIM: 617684

    KEGG: hsa:55646

    STRING: 9606.ENSP00000345917

    UniGene: Hs.425427